Hs3st1 (BC009133) Mouse Untagged Clone

CAT#: MC206482

Hs3st1 (untagged) - Mouse heparan sulfate (glucosamine) 3-O-sulfotransferase 1 (cDNA clone MGC:11450 IMAGE:3155049), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Hs3st1
• Synonyms: 3-OST
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC009133
  CTGCGGTCACCGCCGGGTCTCTCTCGGTGAGTGCCTGTGTCCCTTCACCGCTCGCCCGTCCCGGGGTGAG TGTGAATTTGCTCCAAAGGGCGCAGGTACTTGGCGGCGGTGCTGCCCATGGCCCGGCCGGAGAGAGGATT GAGGGCGCCGGACCGGCTGGACGGGTACGCGACTCAGTAATTGGCAACTGGAGATACTCATGTTCCAGTA ATCAAGAGGGAGCCTTGCTGCTACTTCATGATCCAGGCGCGTGTGGCCCAGTGAAGTCCCTGAGCTGTAC AGCATGACCTTGCTGCTCCTGGGTGCGGTGCTGCTGGTGGCCCAGCCCCAGCTTGTGCATTCCCACCCGG CTGCTCCTGGCCCGGGGCTCAAACAGCAGGAGCTTCTGAGGAAGGTGATTATTCTCCCAGAGGACACCGG AGAAGGCACAGCATCCAATGGTTCCACACAGCAGCTGCCACAGACCATCATCATTGGGGTGCGCAAGGGT GGTACCCGAGCCCTGCTAGAGATGCTCAGCCTGCATCCTGATGTTGCTGCAGCTGAAAACGAGGTCCATT TCTTTGACTGGGAGGAGCATTACAGCCAAGGCCTGGGCTGGTACCTCACCCAGATGCCCTTCTCCTCCCC TCACCAGCTCACCGTGGAGAAGACACCCGCCTATTTCACTTCGCCCAAAGTGCCTGAGAGAATCCACAGC ATGAACCCCACCATCCGCCTGCTGCTTATCCTGAGGGACCCATCAGAGCGCGTGCTGTCCGACTACACCC AGGTGTTGTACAACCACCTTCAGAAGCACAAGCCCTATCCACCCATTGAGGACCTCCTAATGCGGGACGG TCGGCTGAACCTGGACTACAAGGCTCTCAACCGCAGCCTGTACCATGCACACATGCTGAACTGGCTGCGT TTTTTCCCGTTGGGCCACATCCACATTGTGGATGGCGACCGCCTCATCAGAGACCCTTTCCCTGAGATCC AGAAGGTCGAAAGATTCCTGAAGCTTTCTCCACAGATCAACGCCTCGAACTTCTACTTTAACAAAACCAA GGGCTTCTACTGCCTGCGGGACAGTGGCAAGGACCGCTGCTTACACGAGTCCAAAGGCCGGGCGCACCCC CAGGTGGATCCCAAACTACTTGATAAACTGCACGAATACTTTCATGAGCCAAATAAGAAATTTTTCAAGC TCGTGGGCAGAACATTCGACTGGCACTGATTTGCCGTCTCCTAGGCTCGGGACTTTTCCTGTTGTTAACT TCTGGTGTACATCTGAAGGGGGGAGGAAAATAATTTTAAAAAGGCATTTAAGCTATAATTTATTTGTAAA ACCCACAAATGACTTCTGTACAGTATTAGATTCACAGTTGCCATATATAGTAGTTATATTTTTCTACTTG TTAAATGGAGGGCGTTTTGTATTGTTTTTCATGGTTGTTAACATTGTGTATATGTCTCTATAATATGAAG GAACTTAACTATTGCACTGAAAAAATAAGAGATTTTTTTTTTCTGGAGACCTCTTTTTTTGTTGTTGTTG TTTTAAATATAATTAACCTGCCTCCAATCCAAAATAGCTCTTTGTTTTCACCTCCTTGTCAAATCTATAA TCTTTTTCTGCTTAAAAAAATTTATTGGTATTAAAAAAAAAAAAAAA
• Restriction Sites: EcoRI-NotI
• ACCN: BC009133
• Insert Size: 936 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC009133, AAH09133
• RefSeq Size: 1657 bp
• RefSeq ORF: 936 bp
• Locus ID: 15476
• Cytogenetics: 5 21.14 cM
• Gene Summary: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to position 3 of glucosamine residues in heparan. Catalyzes the rate limiting step in the biosynthesis of heparan sulfate (HSact). This modification is a crucial step in the biosynthesis of anticoagulant heparan sulfate as it completes the structure of the antithrombin pentasaccharide binding site.[UniProtKB/Swiss-Prot Function]