Kcna5 (BC021787) Mouse Untagged Clone

CAT#: MC206392

Kcna5 (untagged) - Mouse potassium voltage-gated channel, shaker-related subfamily, member 5 (cDNA clone MGC:25248 IMAGE:4527103),, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kcna5
• Synonyms: MGC25248, Kv1.5
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC021787
  GAGAGAGGCAGGGAGCAGGGGCAGCAGCTTCTTGACGTCAGGACCAGCCAAGGGGATCCAGCCAGCTACC CCAGCCAGCCTAGAGCCAGCGGGGGCCCTCGGCTGAAGAGGAAACCGCGAGGCGCGGAGCGTGAGTTGGG GTGTGGGAACCGGTTGTCTGGGGGCGCAGCATGCCCCTTGCCCCCGGGCCATGGAGATCTCCCTGGTGCC CATGGAGAACGGCAGTGCCATGACCCTCAGAGGAGGAGGGGAGGCAGGGGCAAGCTGTGTGCAGAGCCCC AGGGGAGAGTGTGGGTGCCCTCCGACGGCTGGACTCAATAATCAGTCCAAAGAAACATCACCGAGGAGGC GCGCCACACACGAGGATGCGGGCCAGGGTGGGAGGCCTTTGCCCCCTATGCCTCAGGAGCTGCCACAGCC TAGAAGGCCATCTGCTGAGGATGAGGAGGGAGAAGGCGACCCTGGCCTGGGCACAGTGGAGGAGGACCAG GCTCCTCAGGACTCAGGATCACTCCATCACCAGCGGGTCCTCATAAACATCTCGGGTTTGCGCTTTGAGA CGCAGCTGGGCACCCTGGCGCAGTTTCCCAACACCCTCCTGGGGGACCCAGTCAAACGCCTGCGCTACTT CGATCCCTTGAGAAATGAGTACTTCTTCGACCGCAACCGGCCTAGCTTCGATGGCATTTTGTACTACTAC CAGTCTGGGGGCCGCCTGCGCAGGCCGGTCAATGTCTCCCTGGATGTGTTTGCAGATGAGATCCGCTTTT ACCAGCTGGGGGACGAGGCCATGGAGCGCTTCCGGGAGGATGAAGGCTTCATCAAGGAAGAGGAGAAGCC CCTGCCCCGCAATGAGTTCCAGCGCCAGGTGTGGCTTATCTTCGAATACCCAGAAAGCTCTGGGTCAGCA AGAGCCATTGCCATCGTGTCGGTCTTGGTCATTCTCATCTCTATCATCACCTTCTGCTTGGAGACTCTGC CTGAGTTCAGGGATGAACGGGAGCTGTTACGCCACCCCCCAGTGCCGCCGCAGCCCCCAGCCCCTGCCCC AGGGGCAAATGGCAGTGGCTCTGGTGTCCTTTCCTCTGGCCCCACAGTGGCTCCGCTCCTGCCTAGGACA CTGGCTGACCCATTCTTCATCGTGGAGACCACATGTGTGATCTGGTTCACTTTTGAGCTGCTTGTGCGTT TCTTTGCTTGCCCCAGCAAGGCAGAATTCTCTCGGAATATTATGAACATCATTGATATTGTGGCCATCTT CCCCTACTTTATCACCCTGGGCACCGAGCTGGCAGAGCAACAACCAGGGGGTGGTGGTCAGAATGGGCAG CAGGCCATGTCCCTAGCCATCCTCAGGGTGATCCGCCTGGTCCGGGTGTTCCGAATCTTCAAGCTCTCCC GCCACTCCAAGGGACTGCAGATCCTGGGTAAGACCTTACAGGCGTCCATGCGGGAGCTCGGGCTGCTCAT CTTCTTCCTCTTCATCGGAGTCATCCTCTTCTCCAGCGCTGTCTACTTCGCAGAGGCAGACAATCAGGGG TCGCACTTCTCCAGTATCCCGGATGCATTCTGGTGGGCAGTAGTCACTATGACCACTGTAGGCTACGGGG ACATGAGGCCCATCACTGTAGGGGGCAAGATTGTGGGCTCACTGTGCGCCATAGCTGGGGTCCTCACCAT TGCTCTGCCTGTGCCCGTCATCGTCTCCAATTTTAATTACTTCTATCATCGGGAGACAGACCACGAGGAG CAGGCTGCCCTGAAGGAAGAGCAAGGCATCCAGAGGCGGGAGTCTGGGCTGGACACAGGAGGTCAACGGA AGGTCAGCTGCAGCAAGGCCTCCTTCTGCAAGACTGGGGGACCCCTGGAGAGTACTGACAGTATCAGAAG GGGTAGCTGTCCTCTAGAAAAGTGTCACCTAAAGGCCAAGAGCAACGTGGACCTGCGAAGGTCTCTGTAT GCCCTCTGTCTGGACACTAGCCGGGAAACAGATTTGTAAAGAGAGATCAAGGCAGACTTGCAACAGCAGA GCTGGGAACCGGTTCCTCTTGAGCTTGCTTATCTGCTTTACACCATAGAGTATTTAAGCCCACCTGGTCA CCCATCAAAGTCCGGATCCTGTCTTTCCCCGTTTACCCTTCCCACCCTCCTGCCCTCTAACTTCCCCATC CCCCCCCCTTTCCATAACACCAAGGGTCACCTATTTTTAAAAAGTATCGCATTCCATGACGCAGGAGCCG TTGAAGTGGTGAGCATTCACTGTAAGATGGATGTATTCATAGCCAGTTTTCTATACCCAGCAGAGGGATA ACTCAAACAAAATTGACTTTAAATAGCTCAGATCCCAAGAGAGTCCATAGCCCTCTCTATTCCTGTGTTC CAAATTTACTTTACACGTGATTATATTTGTATATGGCAAATATTATTCTTATGGCTGACGAGTGCATTTT GTACAGCAGTGCACATGAAGAATATTTGGGTGTGTTCTTAAGATGTACTTTGAATTATGAAGTCTTGGGG GGGGTCATCATTTCCTGGGACAACCCAGGGGCAGAGACACTAAAGAAGTTGTTCAAGTTTGTGTTCCCAA GCCTCCCATTATTCTGGGATGTACTATTGGTGCTTTGTGTCTAAGGTAGAGAATGTTCTAGAAGAGCAGC AAACCTGCTTTTTTTTTTTATTCCCCTTAAACATATCCTGTGACTTTCTTCACAGAAGCATTTTAAGGAT GTTGGAAGAAGGCTTTTGAGAATTCATTCTTTTCATTTTGATAGCAGGAAATAAAATGTGACTCTGTCGG GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: BC021787
• Insert Size: 1809 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC021787, AAH21787
• RefSeq Size: 2831 bp
• RefSeq ORF: 1809 bp
• Locus ID: 16493
• Cytogenetics: 6 61.35 cM
• Gene Summary: Voltage-gated potassium channel that mediates transmembrane potassium transport in excitable membranes. Forms tetrameric potassium-selective channels through which potassium ions pass in accordance with their electrochemical gradient. The channel alternates between opened and closed conformations in response to the voltage difference across the membrane (PubMed:8226976, PubMed:11349004). Can form functional homotetrameric channels and heterotetrameric channels that contain variable proportions of KCNA1, KCNA2, KCNA4, KCNA5, and possibly other family members as well; channel properties depend on the type of alpha subunits that are part of the channel (By similarity). Channel properties are modulated by cytoplasmic beta subunits that regulate the subcellular location of the alpha subunits and promote rapid inactivation (By similarity). Homotetrameric channels display rapid activation and slow inactivation (PubMed:8226976, PubMed:11349004). May play a role in regulating the secretion of insulin in normal pancreatic islets (By similarity).[UniProtKB/Swiss-Prot Function]