Ift122 (BC066083) Mouse Untagged Clone

CAT#: MC206184

Ift122 (untagged) - Mouse intraflagellar transport 122 homolog (Chlamydomonas) (cDNA clone MGC:90124 IMAGE:30355922), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ift122
• Synonyms: C86139; sopb; Wdr10
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC066083
  GAAAGAAGCCTGTTGCTGCCGAGGCTGGGAGGAGGGGCCCGAAGCGTGAGGGAAACGGCGATGAGGGCCG TGTTGACGTGGAGAGACAAAGCGGAGCAGTGTATCTACGACCTCGCATTTAAGCCTGATGGAACACAACT GATTCTGGCTGCAGGAAACAGATTGCTGGTTTATGACACCTCTGATGGCACCTTACTTCAGCCCCTGAAG GGACACAAAGACACCGTGTACTGTGTGGCGTATGCAAAGGATGGCAAGCGCTTTGCATCTGGATCTGCTG ACAAGAGCATCATTATCTGGACCTCAAAACTTGAAGGCATTCTGAAGTACACGCACAACGACTCTATTCA GTGTGTCTCCTACAATCCTGTTACCCACCAGCTGGCATCTTGTTCCTCCAGTGACTTTGGGTTATGGTCT CCTGAGCAGAAGTCTGTGTCCAAGCACAAATCCAGCAGCAAGATCACGTGCTGCAGCTGGACAAACGATG GTCAGTACCTGGCTCTGGGCATGGCCAATGGCATCATCAGTATACGGAACAAGAATGGTGAAGAGAAAGT GAAGATCGAGCGCCCTGGGGGCTCCCTCTCCCCAATATGGTCCATCTGCTGGAACCCTTCAAGAGAGGAA CATAATGACATCCTGGCTGTAGCTGACTGGGGACAGAAACTTTCCTTCTACCAGCTGAGTGGAAAACAGA TTGGGAAGGATCGGCCACTGAACTTTGACCCCTGTTGCATCAGCTACTTCACTAAAGGAGAGTACATTTT GGTGGGAGGTTCAGACAAGCAGGTATCTCTTTTCACCAAGGATGGAGTGCGACTGGGGACCGTTGGGGAG CAGAACTCCTGGGTGTGGACCTGTAGAGTGAAACCTGACTCCAATTATGTGGTGGTCGGCTGCCAGGATG GCACCATCTCCTTCTACCAGCTCATCTTCAGCACAGTCCATGGGCTCTACAAAGATCGGTATGCCTACAG GGACAGCATGACAGACGTCATCGTGCAGCACCTCATCACAGAGCAGAAAGTTAGGATTAAGTGCAGAGAG CTTGTCAAGAAAATTGCCATCTATAAAAATCGATTAGCTATCCAGCTGCCAGAGAAGATCCTCATCTACG AGCTGTACTCTGAAGACTCGACAGACATGCACTACCGGGTAAAGGAGAAAATTGTCAAGAAGTTTGAGTG CAACCTGCTGGTGGTGTGTGCTGACCACATCATCCTGTGCCAGGAGAAACGGCTACAGTGCCTGTCCTTC AGTGGAGTGAAGGAAAGGGAATGGCAGATGGAATCTCTCATCCGCTACATCAAGGTGATCGGTGGCCCTG CTGGAAGAGAAGGTCTGTTGGTAGGCCTGAAAAACGGCCAGATCCTGAAGATCTTTGTGGACAATCTTTT TGCTATTGTCCTGCTGAAGCAGGCCACAGCCGTGCGCTGCCTGGACATGAGTGCCTCCCGGAACAAGCTG GCTGTGGTGGATGAGAATGACACGTGCCTGGTGTACGACATCCACACGAAGGAACTGCTTTTCCAGGAAC CGAATGCTAACAGCGTGGCCTGGAACACGCAGTGTGAGGACATGCTCTGCTTCTCTGGAGGAGGCTACCT CAACATCAAGGCCAGCACCTTCCCAGTGCACCAGCAGAAGCTGCAGGGCTTTGTGGTTGGCTACAACGGC TCCAAGATCTTCTGCCTTCATGTCTTCTCTATGTCTGCCGTGGAGGTGCCACAGTCCGCTCCAATGTACC AGTACCTCGATAGGAAGATGTTCAAAGAAGCATATCAGATCGCTTGCTTGGGAGTGACAGACGCTGATTG GCGTGAACTGGCCATGGAAGCTCTAGAAGGACTAGAGTTTGAGACGGCAAGGAAGGCCTTCACCAGAGTC CAGGACCTTCGATACCTAGAACTGATCAGCAGTATTGAGGAGAGGAAGAAGAGGGGAGAGACCAACAATG ACCTGTTTCTGGCAGATGTGTTTTCCTACCAGGGGAAGTTTCATGAAGCCGCCAAACTATACAAGAGGAG CGGGCATGAGAACCTCGCTCTCGACATGTATACTGACCTCTGCATGTTTGAGTACGCCAAGGATTTCCTT GGATCTGGAGACCCTAAAGAAACGAAGATGCTCATCACCAAGCAGGCCGACTGGGCCAGAAACATCAATG AGCCCAAAGCTGCAGTGGAGATGTACATCTCAGCAGGGGAGCACGCCAAGGCCATAGAGATCAGCGGCAG CCATGGCTGGGTGGACATGTTGATAGACATTGCTCGGAAGCTGGACAAGGCTGAACGGGAGCCCCTGCTC ATGTGTGCTTGCTACTTTAAGAAGCTGGACAGTCCTGGCTATGCTGCTGAGACCTACCTGAAGATCGGGG ACCTGAAGTCTCTGGTGCAGCTGTATGTGGACACCAAGCGCTGGGATGAGGCCTTTGCGTTAGGTGAGAA GCACCCGGAGTTCAAGGATGACGTCTACGTGCCCTACGCGCAGTGGCTGGCGGAGAACGACCGCTTTGAG GAAGCCCAGAAAGCCTTCCACAAGGCCGGGAGGCAGGGAGAAGCAGTCCGGGTGCTGGAGCAGCTCACGC ACAATGCTGTGGTGGAGAGCAGGTTTAACGACGCTGCCTATTATTACTGGATGCTGTCCATGCAGTGCCT CGACATGGCTCAAGATCCTGCCCAGAAGGACGCGATGCTGGACAAGTTTCACCACTTCCAACACCTGGCT GAGCTGTACCATGGCTATCAAACCATTCACCGATACACGGAGGAGCCATTCAGCTTTGACCTCCCTGAAA CCCTCTTCAACATCTCCAAGTTCTTGCTGCACAGCCTGACCAAGGCCACCCCCTTGGGTATCTCCAAAGT GAATACACTCTTCACACTGGCTAAGCAGAGCAAGGCTCTGGGTGCTTACAAGCTGGCCCGGCATGCCTAT GACAAGCTACGGGGGCTGCAGATCCCCGCCAGGATCCAGAAGTCCATTGAGCTGGGCACCCTGACCATCC GCTCCAAGCCCTTCCATGACAGTGAGGAGCTGGTACCCTTGTGCTACCGCTGTTCTACCAACAACCCGCT GCTGAACAACCTTGGCAACGTCTGCATCAACTGCCGCCAGCCCTTCATCTTCTCCGCCTCTTCCTATGAG GTGCTGCACCTGGTGGAATTCTACCTGGAAGAGGGGATCACTGACGAAGAAGCTGTTGCCCTCATCGATC TGGAGGCACCAAGACACAAGAGGGAGGGCAAGTGGCGAGAGACCTCAAGCAACAATTCCCAGACGTTGAA GCTGGACGAGACCATGGACTCCATAGGAGAAGATGACCCATTTACAGCCAAGCTGAGCTTTGAGCAAGGT AGCTCTGAGTTTGTGCCTGTGGTGGTGAACCGGTCAGTACTACGCTCCATGAGCCGAAGAGATGTTCTCA TCAAGCGCTGGCCCCCGCCGCTGCAATGGCAATATTTCCGCTCGCTGTTGCCTGATGCCTCCATCACCAT GTGCCCCTCCTGCTTTCAGATGTTCCATTCAGAGGACTACGAACTGCTTGTGCTCCAGCACGCCTGCTGC CCTTACTGCCGAAGGCGTATCGACGACACTGGCCCGTGAGCAGCGACAGCCTCTGGGACCACGGCTCCCA TGGTCTCCTTGCTGTCCCCAGACTGTTGGGAGAAGGAATTAAACCCTCAGAACACGTCTCCTGCCCAGAT GAAGTTTGTATGTTGCAAAGGGGGGGGGGGCTGCCTTGTGTAACCACAGATTTCCTATTTAGGCATTTCA TTCCTTGTAAATAGCACCAGGAGACGGGGAAGAGAACACACACATATTTTCTAAGAAAAAAAAAAAATCT GTTACTTTTAGAAGGGCTCATGTGTTCATAGCAGCCTGCTGGAGTCCGTAGATCCGTCAGACCATTACCG TGCACACGTTGCCAACAGATTGCCTCAATAACGACAAATAAAAGGGCTACTCGTTTTCATCAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: BC066083
• Insert Size: 3549 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC066083, AAH66083
• RefSeq Size: 4059 bp
• RefSeq ORF: 3549 bp
• Locus ID: 81896
• Cytogenetics: 6 E3
• Gene Summary: As a component of the IFT complex A (IFT-A), a complex required for retrograde ciliary transport and entry into cilia of G protein-coupled receptors (GPCRs), it is required in ciliogenesis and ciliary protein trafficking (By similarity). Involved in cilia formation during neuronal patterning. Acts as a negative regulator of Shh signaling. Required to recruit TULP3 to primary cilia (PubMed:19000668, PubMed:21209331).[UniProtKB/Swiss-Prot Function]