Tnfaip3 (BC054806) Mouse Untagged Clone

CAT#: MC206177

Tnfaip3 (untagged) - Mouse tumor necrosis factor, alpha-induced protein 3 (cDNA clone MGC:65662 IMAGE:6838303), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tnfaip3
• Synonyms: A20; Tnfip3
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC054806
  GTTGGCAAAGCATACAACTGAAAGGCAAAAAAGGGAACTGCCCAGTCTGTAGTCTTCGGAGCCGGAGGCG GAGCGGTGAGCCAGGCCGCCGAAAGACGTGCACACCCCCTCAGCCAGCGAGCCCGATCGGGTGTCCATGG GGCTCCGCCTCGGCCCGACCCCCACCACGACCCGGACCACGATCCCGGGCTGCGGCGCGCGCCAGTGACG GCCGGCCGGCCACTTGGGCTCTGCGAGGGCTCCGCGCGGCCCCAAGAGGCCTTGTCGAGGACCATGGCTG AACAACTTCTTCCTCAGGCTTTGTATTTGAGCAATATGCGGAAAGCTGTGAAGATACGAGAGAGAACCCC AGAAGACATTTTCAAACCTACCAATGGGATCATCTATCACTTTAAAACCATGCACCGATACACGCTGGAG ATGTTCAGAACATGCCAGTTTTGCCCACAGTTCCGAGAGATCATCCACAAAGCACTTATTGACAGAAGTG TCCAGGCTTCCCTGGAAAGCCAGAAGAAGCTCAACTGGTGTCGTGAAGTCAGGAAGCTCGTGGCTCTGAA AACCAATGGTGATGGAAACTGCCTCATGCATGCAGCTTGTCAGTACATGTGGGGTGTTCAGGATACTGAC CTGGTCCTGAGGAAGGCCCTCTGCAGCACCCTTAAGGAGACAGACACTCGGAACTTTAAATTCCGCTGGC AGCTGGAATCTCTGAAATCTCAGGAATTTGTGGAAACAGGACTTTGCTACGACACTCGGAACTGGAATGA CGAATGGGACAACTTGGTCAAAATGGCATCAGCAGACACACCTGCAGCCCGAAGTGGACTTCAGTACAAT TCCCTGGAAGAAATCCACATATTTGTCCTCAGCAACATCCTCAGAAGACCCATCATTGTCATTTCAGACA AAATGCTAAGAAGTTTGGAATCTGGTTCCAATTTTGCTCCTTTGAAAGTGGGTGGGATTTATCTGCCTCT TCACTGGCCTGCCCAGGAGTGTTACAGATATCCCATCGTCCTAGGCTATGACAGCCAGCACTTTGTACCC CTGGTGACCCTGAAGGACAGTGGACCTGAACTTCGCGCTGTTCCACTTGTTAACAGAGACCGGGGTAGGT TTGAAGACTTAAAAGTTCACTTCTTGACAGATCCTGAGAATGAGATGAAGGAAAAGCTTCTAAAGGAGTA CTTGATAGTGATGGAGATCCCTGTGCAAGGCTGGGACCACGGCACGACTCACCTGATCAACGCTGCAAAA TTGGATGAAGCTAACTTACCCAAAGAAATAAATTTGGTAGACGATTACTTTGAGCTTGTTCAGCACGAAT ACAAGAAATGGCAGGAGAACAGCGATCAGGCCAGGAGAGCGGCACATGCGCAGAACCCCTTGGAGCCTTC CACACCCCAGCTATCACTCATGGATATAAAATGTGAGACACCCAACTGTCCTTTCTTCATGTCCGTGAAC ACTCAGCCTTTATTCCACGAATGCTCAGAGAGGCGCCAAAAGAATCAGAGCAAGCTCCCAAAGCTGAACT CGAAGCTAGGCCCTGAAGGACTCCCAGGCGTGGGACTTGGCTCCTCAAACTGGAGCCCCGAGGAAACCGC TGGAGGACCTCATTCAGCCCCACCCACAGCACCCAGCCTTTTTCTCTTCAGTGAGACCACTGCAATGAAG TGCAGGAGTCCTGGGTGCCCTTTTACTTTGAATGTGCAGCATAATGGATTCTGTGAGCGTTGCCACGCCC GGCAGATTAATGCCAGCCACACCGCAGACCCTGGAAAGTGCCAAGCCTGCCTTCAGGATGTCACTCGGAC CTTTAATGGCATCTGCAGTACCTGTTTCAAAAGGACTACAGCAGAGCCCAGCTCCAGCCTCACTTCCAGT ATCCCTGCCTCCTGTCACCAACGCTCCAAGTCTGACCCCTCACAACTCATCCAAAGTCTCACTCCACACT CTTGCCACCGGACTGGAAATGTCTCTCCTTCTGGCTGCCTCTCCCAGGCTGCACGGACTCCAGGAGACAG AGCAGGGACAAGCAAGTGCAGGAAAGCTGGCTGCATGTATTTTGGGACTCCAGAAAACAAGGGCTTTTGC ACTCTATGTTTCATCGAATACAGAGAAAATAAGCAGTCTGTTACTGCCTCTGAGAAAGCTGGTTCCCCGG CCCCCAGGTTCCAGAACAATGTCCCGTGCCTGGGCAGGGAGTGCGGCACACTCGGAAGCACCATGTTTGA AGGGTACTGTCAGAAGTGTTTCATCGAAGCTCAGAACCAGAGATTCCATGAAGCAAGAAGAACGGAAGAA CAGCTGAGATCAAGCCAGCATAGAGACATGCCTCGAACTACACAGGTAGCCTCAAGGCTGAAATGTGCCC GGGCCTCCTGCAAGAACATTCTGGCCTGTCGCAGTGAGGAACTCTGTATGGAGTGCCAGCACCTAAGCCA ACGAGTAGGTTCTGTGGCCCACCGGGGTGAGCCCACGCCTGAAGAGCCCCCTAAACAGCGCTGCCGGGCC CCTGCTTGTGATCACTTTGGCAATGCCAAGTGTAATGGTTACTGCAATGAGTGCTACCAGTTCAAGCAGA TGTATGGCTAAGTGCGAACACATTGACAGGTCCAGCAAGAAGGAGCCTCAAACTGCCATTCACAGTGGTA CTGTGATCCAGACCCCTGAGTGCCACAGGAAGGGTAGGCCCTGGGACAGTGGCTTCAAGTGTCTTTGAGC AAGTAGGCAAGATAAGCTCTTCCTTCGTCACAGCCATGACCATCAGGTTAGTAACCGAGACTTTGCCCTG GTGAGCTGAGAGTAGACCTGGTTACCGGCAGTGCATGATGTCAGGGTCAGCCTGAAGCTATTGCTGCTCG TCTACCAGGAGGATGTGGAGATCTTTAGACACGAAATGCGTGCTAGGACTGGCAGCAGTTCCTGAGCCTG GCTGCCAGGCAACAGGAAGAAAGGCGTATGGGAAAGTAGGATGGAGACTGGAACCATCCTACTGCTGCCA GCTGGAAGCTTTTCTCAGAGAGCCAAAGCTGAATTCTCAGAGCTCCAAGGCCCTCCACAAGATGCTAGAG GAAGCTCAGGGAAAAACAGGACGTAGGCGGAGCACATCTGTGGTCAATGGGCTTCCCTATCTGCCGACTT GGCTCCCAAGGTTCTGCAGGAAAGCAGACCTGCACACAGATCCCGTGGCTGCTGAAAAGACACATGTTCA CATGGAGACAAACCAAGCTGCTCGCTCTCGGCTGTATGCACCTTCTAGACAGCAGAACGTCTGGTGGGAA GACATGCCAGTGTTGGCCATTGTCTCCCATTCCAAAGAGAATAACTTTGAACTGAGGGTGTGTATCAGAG TATGTACATATGTAATATACCCTTATATTATGTATGAGGGATTTAAAATATTGAAATGGTTTCCTAGTGG TAGTAGGAAGACTGAATAAGAACAACCTATTTTCTGGTTGTTGTCAGCTTGCCCCTGTCTATACAGCTTG TTACGTGAATTCAACAAGCTGTCTTCTTAACGGGAGCCCTCATTCTGGTTTATTTATTATGAACTTCCAA GTTATTTAAATGAAGATTTCCTTCTGCAGTATTTTCTTGAGATAACATATTTGCTTTCCACCATGTCCTG GGGGCCTGGTTAGTCCCTGGCCATGGAGTGTGGTGGGATGGCTGTCTTCTCTCTCCTTGTTACCTCCCTA GGAAAGAAGGGGTTGCTTTATGGATAAGCCTGTAGAATCGGCTGCTTCCTATGACTCCCTGGCTGCACAA GAAGAAATTGTCTGTCTTCCATATATGCTTCTAAGTATCCTACCTCTTTGAAGAAAAGATAGGGATGGAG TGAGGTGACAGTGGGAAGGGACACAACTACATGGCCTTTCATGACCCTGGCTTACTGTGTAAATGCTGTG CCCTTGGGTACTGGATAGGAACACTGAGAAAGCAAAGCCAATAGCCGTGAGGACGAGGAAGTTTCTGCCA CTGCTTGCAGCTTTTCTGTGTGCTTTGGACTTGCTTTAGGAGGGAAATATAGCTTTGTAGAGATAAAGGC TGACATCTGGGGTCTAGCCTAGCACCTGAAGATACTTGTGATGTCTAACTCTGCATAGCCTTTTCTCTTC ATATCTGCGGCCTATCTTTTCCCCTTACATATGTCTTTGTCCTTCTTTCCCAAAGATGTCAAATACAATG CAGCATTTTCATCTTGTTCTTTCAAAGTTAATAGTCTATTATTTTATGTCTATTGATTTTCATTGTTAAT GTGAAATGGTTATTTATACTTATAGAAAGTTTGTGAAATTTGCACAGTTGTCTTTCAAAGGATAGGATCT ATCTTTTTTGGACTTCAGATTGTTTTAAAGAACTGTAACTTCACAAAAGTCAATATTAAAAAATAAATTA TTTAAGAACAAAAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: BC054806
• Insert Size: 2328 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC054806, AAH54806
• RefSeq Size: 4437 bp
• RefSeq ORF: 2328 bp
• Locus ID: 21929
• Cytogenetics: 10 8.08 cM
• Gene Summary: Ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities. Involved in immune and inflammatory responses signaled by cytokines, such as TNF-alpha and IL-1 beta, or pathogens via Toll-like receptors (TLRs) through terminating NF-kappa-B activity. Essential component of a ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. In cooperation with TAX1BP1 promotes disassembly of E2-E3 ubiquitin protein ligase complexes in IL-1R and TNFR-1 pathways; affected are at least E3 ligases TRAF6, TRAF2 and BIRC2, and E2 ubiquitin-conjugating enzymes UBE2N and UBE2D3. In cooperation with TAX1BP1 promotes ubiquitination of UBE2N and proteasomal degradation of UBE2N and UBE2D3. Upon TNF stimulation, deubiquitinates 'Lys-63'-polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. Deubiquitinates TRAF6 probably acting on 'Lys-63'-linked polyubiquitin. Upon T-cell receptor (TCR)-mediated T-cell activation, deubiquitinates 'Lys-63'-polyubiquitin chains on MALT1 thereby mediating disassociation of the CBM (CARD11:BCL10:MALT1) and IKK complexes and preventing sustained IKK activation. Deubiquitinates NEMO/IKBKG; the function is facilitated by TNIP1 and leads to inhibition of NF-kappa-B activation. Upon stimulation by bacterial peptidoglycans, probably deubiquitinates RIPK2. Can also inhibit I-kappa-B-kinase (IKK) through a non-catalytic mechanism which involves polyubiquitin; polyubiquitin promotes association with IKBKG and prevents IKK MAP3K7-mediated phosphorylation. Targets TRAF2 for lysosomal degradation. In vitro able to deubiquitinate 'Lys-11'-, 'Lys-48'- and 'Lys-63' polyubiquitin chains. Inhibitor of programmed cell death. Has a role in the function of the lymphoid system. Required for LPS-induced production of proinflammatory cytokines and IFN beta in LPS-tolerized macrophages.[UniProtKB/Swiss-Prot Function]