Timm22 (BC055279) Mouse Untagged Clone

CAT#: MC206043

Timm22 (untagged) - Mouse translocase of inner mitochondrial membrane 22 homolog (yeast) (cDNA clone MGC:66904 IMAGE:5688754), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Timm22
• Synonyms: Tim22
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC055279
  TGAAAGGTACTAGAGCCCCTACGGTCATGGCGGCTACCGCCCCCAAGGCCGGGGGTTCAGCTCCTGAGGC AGCGGGTTCTGCCGAAGCTCCGTTGCAGTACAGCCTTCTTCTTCAGTACCTGGTGGGCGACAAGCGTCAG CCCCGGCTTCTGGAGCCTGGCAGCCTGGGCGGGATCCCGAGTCCTGCCAAGAGCGAGGAGCAGAAGATGA TCGAGAGGGCAATGGAAAGTTGCGCCTTCAAGGCTGTGCTGGCCTGTGTAGGAGGGTTTGTCTTGGGAGG CGCGTTTGGTATCTTTACTGCTGGCATTGATACCAACGTAGGCTTTGACCCAAAGGACCCTTACCGGACA CCAACTGCAAAAGAAGTCCTGAAAGACATGGGACAGAGAGGAATGTCCTATGCCAAAAACTTTGCCATTG TGGGCGCCATGTTTTCATGTACTGAGTGTCTGGTAGAGTCTTACCGGGGAAAGTCGGACTGGAAGAACAG CGTCATCAGTGGCTGCATCACTGGCGGAGCCATCGGCTTCCGAGCTGGAGTAAAGGCCGGGGCCATAGGT TGTGGAGGGTTTGCTGCTTTCTCTGCTGCAATCGATTATTACCTACGGTGAAAGAAACAGCCGGGAAGGA AAGAGGACGCCAGCCACCTTCAGAGCTGCTCTGTGGAGCAGTTTCTACACACTTGGACTGCTTCAGGGGT GAAGCCAAGTGCATCCCTCTACAGTTGATGGTGTGAAGGACACACCTCGCTGCTCGATCGCTCTCTGCAG CCATTGGAACAACCATACTTTTCCACTTGAATTCCAACCAGGTTTTTCAGGGGAGGTGGTATGGCAGAGT GTCTCCTTGCAGTAAACAGATCAGGAGGCAGTGTGTGGACTGTTACGCATTAAGCTTTGAACCAGGCTCA GTTCTGACCTGGCTCAGCTCCCCAGCTTTGAATAGGGAAGTGGCAGTGTTTGCCCATAGCCGGGAAGCTC TTCTTCCTTCTGTGTGCAAGTATCCATCATGAGTCCCAGGGCAGGGCAAAGTAAACTTATGCCATCGAGG CAATTATGTGATTTCAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: BC055279
• Insert Size: 585 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC055279, AAH55279
• RefSeq Size: 1091 bp
• RefSeq ORF: 585 bp
• Locus ID: 56322
• Cytogenetics: 11 B5
• Gene Summary: Essential core component of the TIM22 complex, a complex that mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. In the TIM22 complex, it constitutes the voltage-activated and signal-gated channel. Forms a twin-pore translocase that uses the membrane potential as external driving force in 2 voltage-dependent steps (By similarity).[UniProtKB/Swiss-Prot Function]