Nelfcd (NM_020580) Mouse Untagged Clone

CAT#: MC205703

Nelfcd (untagged) - Mouse TH1-like homolog (Drosophila) (Th1l), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nelfcd
• Synonyms: 2410003I03Rik; C77797; Th1; Th1l
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC006959
  CACGCGCAGTGAGGAGACGCGGCAGATATGGCGGGTCCGGCGCCGGGTACAATTATGGGAGAAGACTACT TCGGGAATGCCTCAGAGTGGGGCGAAGAGGCAGACGGCGGCCAGCATCAGGAAGATGATTCCGGAGAAGT AGAGGACGATGCTGAGGTGCAGCAGGAATGCCTGCATAAGTTTTCCACCCGGGACTACATCATGGAGCCC TCCATCTTCAACACTCTGAAGAGGTATTTCCAGGCAGGAGGGTCTCCAGAAAACGTCATCCAGCTCTTAT CTGAGAACTACACGGCAGTGGCCCAGACAGTGAACCTGCTGGCTGAGTGGCTCATTCAGACAGGCGTTGA GCCTGTGCAGGTGCAGGAGACTGTGGAAAACCACTTGAAGAGTTTGCTGATCAAGCACTTCGACCCTCGG AAAGCAGATTCTATTTTCACTGAAGAAGGAGAGACCCCAGCTTGGCTTGAGCAGATGATCGCACACACAA CATGGAGAGATCTGTTTTATAAGTTGGCCGAGGCCCATCCTGACTGTTTGATGCTCAACTTCACTGTGAA GCTCATTTCTGATGCGGGGTACCAAGGGGAGATCACCAGCGTGTCCACAGCCTGCCAGCAGTTGGAAGTG TTCTCCAGAGTGCTTCGGACCTCTCTAGCCACGATCTTGGATGGGGGAGAAGAAAACCTTGAGAAAAATC TCCCTGAGTTTGCAAAGATGGTATGCCATGGCGAGCACACGTACCTGTTTGCACAGGCCATGATGTCCGT GCTGGCACAGGAGGAACAGGGTGGTTCTGCAGTGCGCAGGGTCGCTCAGGAAGTCCAGCGCTTCGCCCAG GAGAAGGGCCATGATGCCAGTCAGATCACGCTGGCCTTGGGCACAGCTGCCTCCTACCCCCGAGCCTGCC AAGCGCTTGGCGCCATGCTGTCCAGAGGAGCCCTGAATCCTGCAGACATCACGGTCTTGTTCAAGATGTT CACCAGCATGGACCCTCCTCCCGTGGAACTCATCCGGGTGCCGGCCTTCCTGGACCTGTTCATGCAGTCG CTCTTCAAGCCAGGGGCCAAGATCAACCAGGACCACAAACACAAGTACATCCACATCCTGGCTTACGCTG CCAGTGTGGTTGAGACCTGGAAGAAGAACAAGCGGGTCAGCATTGGCAAAGACGAGCTCAAGTCCACGTC AAAGGCTATCGAGACTGTCCACAACCTGTGCTGCAACGAGAACAAAGGGGCCTCTGAGCTGGTGGCTGAG CTGAGCACACTTTATCAGTGCATTCGGTTTCCAGTGGTGGCGATGGGCGTGCTCAAGTGGGTAGATTGGA CCGTATCGGAGCCAAGGTACTTCCAGCTGCAGACTGATCACACTCCAGTTCACCTGGCCTTGTTGGATGA GATCAGCACCTGCCACCAGCTCCTGCACCCCCAGGTTCTGCAGCTGCTTGTTAAGCTTTTTGAGACAGAG CACTCCCAGCTAGACGTGATGGAGCAGCTCGAGTTGAAAAAGACCCTGCTGGACAGGATGGTGCACCTGC TGAGCCGCGGCTACGTCCTGCCGGTGGTCAGTTATATCCGCAAATGTCTGGAGAAGCTGGACACGGACAT CTCCCTCATCCGCTACTTTGTCACTGAGGTTCTGGATGTCATCGCCCCTCCGTACACCTCAGACTTTGTG CAGCTTTTCCTCCCCATCTTGGAAAATGACAGCATCGCGGGCACCATCAAGGCAGAGGGCGAGCATGACC CCGTGACGGAGTTCATTGCTCACTGCAAGTCGAACTTCATCGTGGTGAACTGAGCGCATCTGTAACTGAA GCCGAAACTTCCATGCCAGCCTGGGGTGGGGGGACCAGAGGACTCGTTCAGAAAGCTGCTCTGTGAAGAG GCTCAACCAGCCTCCTGGAAGCAGGGTCTGCTGGGAAGAAGCAGGCAACTCTTCTCAAAGGTAGCTTCTG TGGCAGACAGCCCCCCTGTGTCGCAGTGTGGCTCTGTGCTACATGTCACATCCCAGGCGTGTGCCACCTC TGAACAGGGACCCAGTGGGCCTCTGGGTAGTGGGAATCTAGCAGCTGGGACACTTCATGAAGGGTTTATG TAAGTTTCCTTCCTATGACCCAGATGGTCAGTTACTTTAAGTTTCCAAGAGTAATGTTGAGTTGTATGTG TGTGTGTGGGAGTGTGCGTGTGTTGTGTGTGCTAGTCTCGTGTAAATAAAGTACCCAAGCGTGGGAAGCC AAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_020580
• Insert Size: 1749 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC006959, AAH06959
• RefSeq Size: 2265 bp
• RefSeq ORF: 1749 bp
• Locus ID: 57314
• UniProt ID: Q922L6
• Cytogenetics: 2 H4
• Gene Summary: Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II (By similarity). The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex (By similarity).[UniProtKB/Swiss-Prot Function]