Vmp1 (NM_029478) Mouse Untagged Clone

CAT#: MC205671

Vmp1 (untagged) - Mouse vacuole membrane protein 1 (Vmp1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Vmp1
• Synonyms: 3110098I04Rik; 4930579A11Rik; AI787464; mir-21a; ni-2; Tango5; Tmem49
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC004013
  CCAGCGGCGGGTGTGAGAGTCGGTGTTGTGAGCCGCTTCCAGGATCCTGCGATCCGGAGAAGCCGGGGTC TGAGCGGCTCCTCCAGAGTTCCTGATCTATGAAATGGCAGAGAATGGAAAAAATTGTGACCAGAGACGCG TAGCAATGAGTAAGGATCAGCACAATGGAAGTCTCACAGACCCCTCTTCAGTTCATGAGAAGAAGAGAAG GGATCGGGAAGAAAGACAGAATATTGTCCTGTGGAGACAGCCACTCATTACCTTGCAGTATTTCTCTCTG GAAACTCTTGTAGTTTTGAAGGAATGGACCTCAAAATTGTGGCATCGTCAAAGCATTGTGGTGTCCTTTT TACTGCTGCTTGCTGCGCTTGTAGCTACGTATTATGTGGAAGGAGCGCACCAACAGTATGTGCAGCGGAT AGAGAAGCAGTTTCTTTTGTATGCCTACTGGATAGGCCTGGGGATTTTGTCCTCTGTTGGTCTTGGAACA GGACTGCACACCTTTCTGCTTTATCTGGGCCCACATATAGCTTCAGTTACATTAGCTGCTTATGAATGCA ATTCGGTGAATTTCCCTGAGCCACCCTATCCTGACCAGATTATCTGCCCAGAGGAAGAAGGCGCTGAGGG AGCCATTTCTTTGTGGAGTATCATCTCAAAAGTTAGAATTGAAGCCTGCATGTGGGGCATTGGAACAGCC ATTGGAGAGCTGCCTCCATATTTCATGGCCAGGGCAGCTCGCCTCTCAGGTGCTGAACCAGATGATGAAG AGTATCAGGAATTTGAAGAAATGCTGGAACATGCAGAGGCTGCACAAGACTTTGCATCACGGGCTAAACT GGCAGTTCAAAAACTAGTACAGAAAGTTGGATTTTTTGGAATTTTGGCCTGTGCTTCTATTCCAAACCCC CTGTTTGACCTGGCTGGAATAACGTGTGGGCACTTCCTTGTACCTTTCTGGACCTTCTTTGGTGCAACCC TGATTGGGAAAGCAATCATTAAAATGCATATCCAGAAAATATTTGTTATAGTAACTTTCAGCAAGCACAT CGTGGAGCAGATGGTGACTTTCATTGGTGCTGTCCCTGGCATAGGTCCGTCTCTGCAGAAGCCTTTTCAG GAGTACCTGGAGGCGCAGCGGCAGAAGCTTCATCACAGAAGTGAAGCGGGCACACCGCAGGGAGAAAACT GGTTATCCTGGATGTTTGAGAAGCTGGTGGTTGCAATGGTGTGTTACTTTGTCCTGTCTATTATTAACTC CATGGCACAAAACTATGCCAAACGAATCCAGCAGCGCTTGAACTCAGAGGAGAAAACTAAATAAGCAGAG AGTTTTTATCTGCAGAAGTTAACATGGTGGAGTCCTGCCTTACACTGGGAGGGCTCTAACCAGGAAGGAA GGTCCCCGTTTCCAACCTGTGTTGATTTTTAAAACATTCTTCCTGAAAGCAGTTTAGTCCATATGTTACA CTGACATACTTTATTTGTTAAGGTAAGGTGTCTACACCCTCAGTTCAATCCACGTCGTATTCTTTAGGGT GGATGTGATGTTCTGATGCAAACTTAACAAAAACTGGCCTTCTGATACCTTCACAGGGCCACACAGAACC TTCTGAAGTATGTTAAATATGTCAGGCTTTTTAGGCTTGTCACAAATGATTGTTTTTTTCTAAGTCACCA AATGTATATAAGTTATATATATTGGATAGCAATCTTGCATGTCTATCATGGAACAATTTAATATGCCTTC CTTTCCCACCCTCAAAAAGGCCATTTTATGACGCATTGCACACCCTCTGGGGAAATTGATCTTTAAATTT TGAAACAGTATAAGGAAAATCTGGTTGGTGTCTCACAAATGAGTACGCCATTTTTTATTCTGTATATTTA GAATGAAGTCGTGAAAAACTTTATAAAGACATCTTTGATCATTCCGAAAAAAAAAAAAAAAAAAAAAAAA A
• Restriction Sites: RsrII-NotI
• ACCN: NM_029478
• Insert Size: 1221 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC004013, AAH04013
• RefSeq Size: 1961 bp
• RefSeq ORF: 1221 bp
• Locus ID: 75909
• UniProt ID: Q99KU0
• Cytogenetics: 11 C
• Gene Summary: Stress-induced protein that, when overexpressed, promotes formation of intracellular vacuoles followed by cell death (By similarity). May be involved in the cytoplasmic vacuolization of acinar cells during the early stage of acute pancreatitis (PubMed:17940279). Involved in cell-cell adhesion. Plays an essential role in formation of cell junctions. Plays a role in the initial stages of the autophagic process through its interaction with BECN1. Required for autophagosome formation (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. COMPLETENESS: complete on the 3' end.