Kras (NM_021284) Mouse Untagged Clone

CAT#: MC205663

Kras (untagged) - Mouse v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (Kras), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kras
• Synonyms: AI929937; c-K-ras; c-Ki-ras; K-Ras; K-ras; Ki-ras; Kras-2; Kras2; p21B; ras
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC004642
  CGGACGCGTGGGCGGCAGCGCTGTGGCGGCGGCTGAGACGGCAGGGGAAGGCGGCGGCGGCTCGGCCCGG AGTCCCGCTCCCGCGCCATTTCGGACCCGGAGCGAGCGCGGCGCGGGCCTGAAGGCGGCGGCGGGAGCCT GAGGCGCGGCGGCTCCGCGGCGCGGAGAGAGGCCTGCTGAAAATGACTGAGTATAAACTTGTGGTGGTTG GAGCTGGTGGCGTAGGCAAGAGCGCCTTGACGATACAGCTAATTCAGAATCACTTTGTGGATGAGTACGA CCCTACGATAGAGGACTCCTACAGGAAACAAGTAGTAATTGATGGAGAAACCTGTCTCTTGGATATTCTC GACACAGCAGGTCAAGAGGAGTACAGTGCAATGAGGGACCAGTACATGAGAACTGGGGAGGGCTTTCTTT GTGTATTTGCCATAAATAATACTAAATCATTTGAAGATATTCACCATTATAGAGAACAAATTAAAAGAGT AAAGGACTCTGAAGATGTGCCTATGGTCCTGGTAGGGAATAAGTGTGATTTGCCTTCTAGAACAGTAGAC ACGAAACAGGCTCAGGAGTTAGCAAGGAGTTACGGGATTCCGTTCATTGAGACCTCAGCAAAGACAAGAC AGGGTGTTGACGATGCCTTCTATACATTAGTCCGAGAAATTCGAAAACATAAAGAAAAGATGAGCAAAGA TGGGAAGAAGAAGAAGAAGAAGTCAAGGACAAGGTGTACAGTTATGTGAATACTTTGTACTCTTTCTTAA GGCACACTTAAGTAAAAGTGTGATTTTTGTACATTACACTAAATTATTAGCATTTGTTTTAGCATTACCT AATCTTTTTTTTTTCTTCTGTTCGTGCAAACTGTCAGCTTTTATCTCAAATGCTTATTTTAAAAGAACAG TGGAAACCTTCTTTTTTCTAAGTGCCAGTATTCCCTGGGTTTTGGACTTAAACTAGCAATGCCTGTGGAA GAGACTAAAGACCTGAGACTCTGTCTTGGGATTTGGTGCATGCAGTTGATTCCTTGCTAGTTCTCTTACC AACTGTGAACACTGATGGGAAGCAGGATAATGAAGCTTCCGGACCATCCCTGCTCTGTGTCCATCTACTC ATCCAATGGAGTCATTAGCAGTCAATCGCAGCTTCACTGGACACTGAGGGGTCACAGACTTAGGCTCCCT TTGAGTCACGTCCAGCGTGTCCTAGACTTTATCATCTTTCAGAGGCGTAGGCAGACTGTTCACAAAGGCT TTCTCTAGCTTTCCACTGCAATTAATCTTGGTCACTCCCTCAAATAGTATATTTTTTCTAGAAAAGGGGA AAAATGGAAAAAAAAAAAAAGGCAATGGAAAATGTTGAAATCCATTCAGTTTCCATGTTAGCTAAATTAC TGTAAGATTCCTATAATAGCTTTTCCTGGTAAGGCAGACCCAGTATGAAATAGTAATAACCATTTGGGCT ATATTTACATGCTACTAAATTTTTGTAATAATTCAAACAACTTTAGCATATATAAAAAGTTCTCATAAGA ATTAAGTACAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_021284
• Insert Size: 567 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC004642, AAH04642
• RefSeq Size: 1567 bp
• RefSeq ORF: 567 bp
• Locus ID: 16653
• UniProt ID: P32883
• Cytogenetics: 6 77.37 cM
• Gene Summary: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity (By similarity). Plays an important role in the regulation of cell proliferation (PubMed:6474169, PubMed:1352876). Plays a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes (TSGs) in colorectal cancer (CRC) cells in a ZNF304-dependent manner (By similarity).[UniProtKB/Swiss-Prot Function]