Api5 (NM_007466) Mouse Untagged Clone

CAT#: MC205629

Api5 (untagged) - Mouse apoptosis inhibitor 5 (Api5), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Api5
• Synonyms: AAC-11; AI196452; API-5
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC007133
  GCCCGGCGGCGGCTCCCGGCGTACTAGTGCGGTCGACGGCTCAGATAAGTTCCGTGGAGGCGGCGGTGGC GCCGACGAGGTGTTGGGCGACGGGAGCGGGCCCGGAACTTGGCGCTCAGCATGCCGACGGTGGAGGAGCT GTACCGCAACTACGGCATCCTCGCCGATGCCACGGAGCAAGTGGGCCAGCATAAAGATGCCTACCAAGTG ATACTGGATGGCGTGAAAGGCGGCACCAAGGAAAAGCGATTAGCAGCTCAGTTTATACCAAAATTCTTTA AGCATTTTCCAGAGCTGGCTGATTCTGCTATCAATGCACAGTTAGACCTCTGTGAGGATGAAGATGTGTC AATACGACGTCAAGCAATTAAAGAGCTGCCTCAGTTTGCCACAGGAGAAAACCTTCCTAGAGTAGCAGAT ATACTGACCCAGCTTCTGCAGACAGATGACTCTGCAGAATTTAACCTGGTGAACAATGCTCTGCTAAGTA TATTTAAGATGGATGCAAAAGGAACGTTAGGTGGCTTGTTTAGCCAGATTCTTCAAGGAGAAGACATTGT TAGAGAACGAGCAATTAAATTCCTGTCCACAAAACTCAAGACGCTCCCAGATGAGGTGTTGACTAAGGAA GTGGAGGAACTCATACTCACTGAGTCCAAGAAGGTCCTAGAAGACGTGACTGGTGAAGAGTTTGTTTTGT TCATGAAGATACTGTCTGGGTTGAAAAGCTTACAGACAGTGAGTGGACGACAGCAGCTGGTGGAGCTGGT GGCGGAACAGGCTGACCTGGAGCAGGCCTTCAGCCCCTCAGACCCCGACTGTGTGGACAGACTACTGCAG TGCACGCGGCAGGCTGTGCCTCTCTTCTCCAAAAATGTGCATTCTACAAGATTTGTGACGTATTTCTGTG AGCAAGTTCTCCCTAACCTCAGCACCCTGACCACCCCAGTGGAAGGCCTCGATATACAGCTGGAGGTATT AAAGTTGTTGGCAGAGATGAGTTCATTTTGTGGTGACATGGAAAAACTAGAAACAAATTTAAGAAAACTG TTTGATAAGTTATTGGAGTATATGCCTCTCCCTCCAGAAGAAGCAGAAAATGGGGAGAATGCTGGTAATG AGGAGCCCAAGCTGCAGTTCAGTTATGTGGAGTGCTTATTGTACAGTTTTCATCAGTTGGGGCGAAAACT TCCAGACTTCTTAACAGCCAAACTGAATGCAGAAAAACTCAAGGATTTCAAAATTAGGTTGCAGTACTTT GCACGGGGCCTGCAGGTTTATATCAGACAACTTCGCTTGGCTCTCCAGGGTAAAACAGGCGAGGCCTTAA AAACAGAGGAGAACAAGATAAAAGTTGTTGCACTGAAAATAACAAACAATATCAATGTTTTAATCAAGGA TCTCTTCCACATTCCTCCTTCTTATAAGAGCACAGTAACATTGTCCTGGAAACCTGTGCAGAAAGTTGAG ATTGGGCAAAAGAGAACCAGTGAAGATACAAGTTCAGGTTCACCACCTAAGAAGTCTCCAGGAGGACCAA AAAGAGATGCCAGACAGATTTATAATCCTCCGAGTGGAAAGTACAGCAGCAATCTGAGCAACTTTAATTA TGAGAGGAGCCTTCAGGGGAAATAGAGGTGGCCGAGGTTGGGGAACACGAGGAAATCGAAGTCGAGGAAG ACTCTACTGATTGTGACGTCACATTCTTCAGCATTGTCATGAGATTAATATACTTAAATCTACTACTCAT TGGATTGCCGGGGATGTCCCTTTAAAAGGACTGCTGCCTTCAGCTGAAAATGTAATGTTCTTTCTACCTT TGTATGTATGATCTACTTTTGTAAAAGACCATGGTTGTGTCCAAGGTAAAACCACAACAATATTTTTGGA TGCTTTGTCTGCAGCCTTGACTTGTTTTCACAATATCCTCACTATCCAGACTTCATCTTGTGAATCTTGC TTTTACACATCTTGATGGTTTGTCACTGAGATCTGACTCTATTGGAAAAGTCAGTCTAGCCTGTGATCTG GTGAAGATCATCACTCTTGAAACGTTACTGTTCTCCTCCCCATTCCGTTAGGTTTCTGCCCAACACATGG AGAAGAGTTAAGAGCAGTCTTAACCTTCTGCTTTCATTGTTTTCTAAGGAAGCTGCTAGGCAGTGTTGTC GCATTCCTGCCAAGTCATAGAAATGAAGCACCAGCTTCTCACAGTGTACAGGCGATGCCAGCCTTTTAGA AAGAACTTGCTTTGCATTGCCCCTTCAGTCTTCTCCATATTTTGGGATACAGCTTTGAACACAGGGTCTT ATTATTTGAATAGGAGTACATGTGCATAATATACGTACAGACATAAGCATATGTTGTGTGTGTATATATG CATGCTATGGAACTTGAGTGTACAACCAATCAATACATTAAATTCTCGGAAGGGGTCCTCTGACATAGCG ATTATCCCTTTGAGGCTGAATCCATTACTGACTTGGTATCTAGGTTTTCACTCCAGTAGGGAGGAATTTC AGTCAGCTGAACTTACAGATTATTATTGTTGTTTAAAACTAAAAAACAAAGGCTGTTTTTACAGAGACAA ATTTAAAGACCCTTGTTGGTGAAATACAGCCAGATCTGGTGTACATACAGGTTCCCACAGGATCTGCTAG TGTAATGTAGGATTTGGAGACTTAATAACCAGGGCTACCCAGGAGTGTGACTTGGTGACATAGTACCATA AAAGTTGCTCACTCCGCTTGCTTTTGCCACTTTCAAATTTTAACTTCTCAGGTTATTAATCAGATTATTG TGTAAGTTAGCCAATAGTCTTTAGATTAAGGCAACAGACGGGAGGTTCGTGGAGTGTCTCATGTTGGGCA TTTTTAGTAGCCCAGACTCTGTTCTTCATTTGAATGTTTCACACATTTTTGTTCACAGTTAATCTTCCAA GTTTACTATTCAAATCAGAAATTCAGATGACATTTCTAGTGGTTTGCTGTTTTGGTTTTTTATGTTTTTT GGTTTTTTTGAGGTTTCATTTCTTACACAGGTGTCTTCATCACCATCACTTCTACACTGGGGGAAAAACA ATCTCCTTTGTGAGAATCACTGCACGTATTTATGGCGAAAATATTTCTGAAAGTCTAGAGTGATACAAGT GAGCACAAGAAGTTGGTCAGCTTGCCTATGGAGTGCTGGCAATAAACTCTGAACATTCCACAAGCCTGAG CTGAACCTAGGCTCCCTTGGAAGCTGAACAGACATAGGAACATGGGATTGCCAGCTGAATCCTGTGACCA TCTAGTTATGGACCTCAGAGATAGATCAGCATGGCCTAAAGCCATTTCAAGTACAAAAATGAATTGGACT ATAGCAACATAAATTTGAACACAGTAACTTTTCCTCTTTGCCTGAGGGCATTTGTAAACTCGGCAGAAGT AACTTGACCTCATGTCTACTGCAGAGCTCACTGCATTCACCCCTCCTCATCCTTTGCTTCCTTCCCCTTG CCTAGTCAGTAGTTCATAACTTAGTGTTCCTTTTGCTTCAGAAATCTGAAAGAAAATCTTCATGCCATGA ATCTTTTTCTTCCGCAAGGACACCTGTGTCTCCCTTGTTTAAATAAATGTAAATGCTCCCTTATGCTTTT GAAATAAATTTCCTTTTGTAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_007466
• Insert Size: 1515 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC007133, AAH07133
• RefSeq Size: 3674 bp
• RefSeq ORF: 1515 bp
• Locus ID: 11800
• UniProt ID: O35841
• Cytogenetics: 2 E1
• Gene Summary: Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 3' UTR and uses an alternate splice site in the 3' coding region that results in a frame-shift compared to variant 1. The encoded isoform (2) has a shorter and distinct C-terminus compared to isoform 1.