Pccb (NM_025835) Mouse Untagged Clone

CAT#: MC205562

Pccb (untagged) - Mouse propionyl Coenzyme A carboxylase, beta polypeptide (Pccb), nuclear gene encoding mitochondrial protein, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pccb
• Synonyms: 1300012P06Rik; AI314687; R74805
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC055946
  CCCACGCGTCCGAAAAAAAAAAAAAATGGCGGCGGCGATTAGGATTCGGGCGGTGGCGGCTGGAGCGAGG CTCAGCGTTCTGAATTGCGGCCTCGGCATCACGACCCGCGGCCTTTGCAGCCAGCCGGTTTCAGTTAAAG AGCGCATCGACAACAAGCGCCATGCAGCGTTGCTGGGAGGAGGCCAGCGCCGCATCGACGCGCAGCACAA GCGAGGGAAGCTAACAGCCAGGGAGCGGATCAGTCTTCTGCTGGATCCTGGCAGCTTCGTGGAGAGCGAC ATGTTTGTGGAACACAGATGTGCCGACTTCGGAATGGCTGCTGATAAGAATAAGTTTCCTGGAGATAGTG TGGTCACTGGACGGGGCCGAATCAATGGACGATTGGTTTATGTCTTCAGTCAGGATTTTACAGTTTTTGG AGGCAGTCTGTCAGGAGCACATGCTCAAAAGATCTGCAAAATCATGGACCAGGCCATAACAGTGGGGGCT CCAGTGATCGGGCTCAATGACTCCGGGGGCGCCAGAATCCAGGAGGGGGTGGAGTCCTTGGCTGGCTACG CGGACATCTTCCTGAGGAATGTCACAGCGTCTGGAGTCATCCCGCAGATTTCTCTGATCATGGGCCCGTG TGCTGGTGGGGCCGTCTACTCCCCTGCCCTAACAGACTTCACATTCATGGTGAAGGACACATCTTACCTG TTTATCACTGGCCCTGAAGTTGTGAAGTCTGTTACCAATGAGGATGTCACTCAAGAGCAGCTGGGTGGTG CCAAGACCCACACCACTGTGTCAGGTGTGGCCCATAGAGCTTTCGACAATGATGTTGATGCTCTGTGTAA CCTGCGTGAATTCTTCAATTTCCTGCCCCTCAGCAGCCAGGACCCGGCTCCCATCCGAGAGTGCCATGAC CCCAGTGACCGTCTGGTTCCTGAGCTGGACACAGTTGTCCCTTTGGAGTCAAGCAAAGCCTACAACATGC TGGACATCATCCATGCAGTGATTGACGAGAGAGAGTTTTTTGAGATAATGCCCAGTTACGCAAAAAATAT TGTCGTTGGCTTCGCAAGAATGAATGGGAGGACCGTCGGAATTGTCGGCAACCAGCCCAATGTGGCCTCA GGGTGCTTGGACATTAATTCATCCGTGAAGGGGGCTCGTTTTGTCAGATTCTGTGACGCATTCAACATTC CCCTCATCACTTTCGTTGACGTCCCTGGCTTCCTGCCTGGCACGGCGCAGGAGTATGGGGGCATCATCAG GCATGGTGCCAAGCTGCTCTACGCGTTCGCCGAGGCAACTGTGCCCAAAATCACAGTCATCACCAGGAAG GCCTATGGAGGTGCCTATGACGTCATGAGCTCCAAACACCTTCTTGGTGACACCAACTATGCCTGGCCCA CAGCTGAGATTGCAGTCATGGGAGCAAAGGGTGCTGTGGAGATCATCTTCAAAGGACACCAAGATGTGGA AGCCGCCCAGGCAGAGTATGTGGAGAAGTTCGCCAACCCTTTCCCAGCAGCCGTGAGAGGGTTTGTGGAT GACATCATCCAGCCATCCTCTACTCGTGCTCGGATATGCTGTGACCTGGAAGTCCTGGCCAGCAAGAAGG TCCATCGTCCCTGGAGGAAACATGCAAATATCCCACTGTGAGCAGATGGAAGGGACTAGAGCAACAGTTG AAAAGCTGGCCTTTTGCAGCGTACCCCTCCCTGCCTTTTACAATCATGAAAAAATGGATCTATGTATCTT GGACACTGATTGATTTGCATAAAGAGTTCTTGGGCCTGGAGTAAAACTATGTGCTCTATTAAAATCATTG GTACTTTTTGAATAAATATTTCATTGTTATAGTAAAAATCTCAATCTTAAACAAGAAAGCCAGAAGGAGG CGGGGGATGGAATCGTAGTTGACTGAATCTGGCTCTGGTTCACACTGATGTGGGTATGATTTTTGTGGCC CTCAGAGGTCGACATGCTGGAGCTTGAACTCCGTGTGGGGTGGTGAGAGCAGAGAGATAGTGGGAGATGC TGTGGTCAGCTGGGCTGGGCTCTGTGCTGAAGAGGCTAAGAGTTCTTAGTCTTTCCCCAGCTGCTCGATG GCTATGTGATGTCTCGATATTCCCCCACCCCATGTGGGCTCCATGGTGTGATACACCAGGAGGGTTGTCA CCAGAACTTCACTGATGGTAATGCCTTGCCATTGGATCTCAGAATTATGAGCTAATTAAACCCCAAACCT CTTCATAAAATTAACCTGCCTTCAAGATTTTGTTATAGTAATAAAAGCGGATTAATTCAACATAAAAAAA AAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_025835
• Insert Size: 1626 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC055946, AAH55946
• RefSeq Size: 2323 bp
• RefSeq ORF: 1626 bp
• Locus ID: 66904
• UniProt ID: Q99MN9
• Cytogenetics: 9 E4
• Gene Summary: This is one of the 2 subunits of the biotin-dependent propionyl-CoA carboxylase (PCC), a mitochondrial enzyme involved in the catabolism of odd chain fatty acids, branched-chain amino acids isoleucine, threonine, methionine, and valine and other metabolites. Propionyl-CoA carboxylase catalyzes the carboxylation of propionyl-CoA/propanoyl-CoA to D-methylmalonyl-CoA/(S)-methylmalonyl-CoA (By similarity). Within the holoenzyme, the alpha subunit catalyzes the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain, while the beta subunit then transfers the carboxyl group from carboxylated biotin to propionyl-CoA (By similarity). Propionyl-CoA carboxylase also significantly acts on butyryl-CoA/butanoyl-CoA, which is converted to ethylmalonyl-CoA/(2S)-ethylmalonyl-CoA (By similarity). Other alternative minor substrates include (2E)-butenoyl-CoA/crotonoyl-CoA (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).