Gnptg (NM_172529) Mouse Untagged Clone

CAT#: MC205554

Gnptg (untagged) - Mouse N-acetylglucosamine-1-phosphotransferase, gamma subunit (Gnptg), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gnptg
• Synonyms: 6430527N14Rik; A830081F19; AU067667; AU067744; Mdcp1; Tce7
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC055872
  GAGCAATGGCCGGGCGGCTGGCTGGCTTCCTGATGTTGCTGGGGCTCGCGTCGCAGGGGCCCGCGCCGGC ATGTGCCGGGAAGATGAAGGTGGTGGAGGAGCCTAACACATTCGGGCTGAATAACCCGTTCTTGCCCCAG GCAAGCCGCCTTCAGCCCAAGAGAGAGCCTTCAGCTGTATCCGGGCCCCTGCATCTCTTCAGACTTGCTG GCAAGTGCTTTAGCCTAGTGGAGTCCACGTACAAGTATGAATTCTGCCCTTTCCACAACGTCACCCAGCA CGAGCAGACCTTCCGCTGGAATGCCTACAGCGGGATCCTTGGCATCTGGCATGAGTGGGAAATCATCAAC AATACCTTCAAGGGCATGTGGATGACTGATGGGGACTCCTGCCACTCCCGGAGCCGGCAGAGCAAGGTGG AGCTCACCTGTGGAAAGATCAACCGACTGGCCCACGTGTCTGAGCCAAGCACCTGTGTCTATGCATTGAC ATTCGAGACCCCTCTTGTTTGCCATCCCCACTCTTTGTTAGTGTATCCAACTCTGTCAGAGGCCCTGCAG CAGCGCTGGGACCAGGTGGAACAGGACCTGGCAGATGAACTGATCACACCACAGGGCTATGAGAAGTTGC TAAGGGTACTTTTTGAGGATGCTGGCTACTTAAAGGTCCCAGGAGAAACCCATCCCACCCAGCTGGCAGG AGGTTCCAAGGGCCTGGGGCTTGAGACTCTGGACAACTGTAGAAAGGCACATGCAGAGCTGTCACAGGAG GTACAAAGACTGACGAGTCTGCTGCAACAGCATGGAATCCCCCACACTCAGCCCACAGAAACCACCCACT CTCAGCACCTGGGTCAGCAGCTCCCCATAGGTGCAATCGCAGCAGAGCATCTGCGGAGTGACCCAGGACT ACGTGGGAACATCCTGTGAGCAAGGTGGCCACGAAGAATAGAAATATCCTGAGCTTTGAGTGCCCTTTCA CAGAGTGAACAAAACTGGTGTGGTGTAGACACAGCTTCTTTTGGCATATTCTAGATCAGACAGTGTCACT GACAAACAAGAGGGACCTGCTGGCCAGCCTTTGTTGTGCCCAAAGATCCAGACAAAATAAAGATTCAAAG TTTTAATTAATTCCATACTGATAAAAAATAACTCCATGACTTCTGTAAACCATTGCATAAATGCTATTGT AAAAAAATTTAAACAAATGTTAACAACTTTAACAATTCACTAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_172529
• Insert Size: 924 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC055872, AAH55872
• RefSeq Size: 1246 bp
• RefSeq ORF: 924 bp
• Locus ID: 214505
• UniProt ID: Q6S5C2
• Cytogenetics: 17 A3.3
• Gene Summary: Non-catalytic subunit of the N-acetylglucosamine-1-phosphotransferase complex, an enzyme that catalyzes the formation of mannose 6-phosphate (M6P) markers on high mannose type oligosaccharides in the Golgi apparatus. Binds and presents the high mannose glycans of the acceptor to the catalytic alpha and beta subunits (GNPTAB). Enhances the rate of N-acetylglucosamine-1-phosphate transfer to the oligosaccharides of acid hydrolase acceptors.[UniProtKB/Swiss-Prot Function]