Hs2st1 (NM_011828) Mouse Untagged Clone

CAT#: MC205511

Hs2st1 (untagged) - Mouse heparan sulfate 2-O-sulfotransferase 1 (Hs2st1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Hs2st1
• Synonyms: 2OST; AW214369; Hs2st; mKIAA0448
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC025443
  CCACGCGTCCGGCGAGGGAAGAGGGCGTGCGGCCGTGGGGTCCCGAAGCCAGGGGGCAGCGGCAGCGGCA GGAGGGCGCCAGGGCCGTCGAGTGGGCCCGGGAGAAGCGTGCATCAGGCGGCCGGCGCTGGCTCCCGCGG GCGGGAGACCGGAGAGGAGGAGGGGGTCGCTGCGGCGCGGCAGTCCGCGCAGCTCCCGGGTCGGGGTCTC CTCCCGGCGCTTCGGTCGCCCTCGGGGCCCCGCTCCCCGCCCCCCGCGGTATGTCTTGATCGCGAGCGGC GGGTTTCATGGGGCTCCTCAGGATTATGATGCCGCCCAAGTTGCAGCTGCTGGCGGTGGTGGCCTTCGCG GTGGCGATGCTCTTCTTGGAGAACCAGATCCAGAAGCTGGAGGAGTCCCGGGCGAAGCTAGAAAGGGCAA TTGCAAGGCACGAAGTCCGAGAAATTGAACAGCGCCATACAATGGATGGCCCTCGGCAGGATGCAACTCT GGATGAAGAAGAAGACATCATCATCATTTATAACAGAGTGCCCAAAACTGCAAGCACCTCGTTCACCAAT ATAGCCTACGACCTGTGTGCAAAGAATAGATACCATGTTCTTCACATCAACACTACCAAAAATAACCCAG TAATGTCATTGCAAGATCAGGTGCGCTTTGTGAAGAACATAACCACCTGGAACGAGATGAAGCCAGGCTT CTATCACGGACACATCTCTTACTTGGATTTTGCAAAATTCGGTGTGAAGAAGAAACCGATTTATATCAAT GTCATCAGGGACCCTATTGAGAGGCTAGTTTCCTACTATTACTTCCTGAGGTTTGGAGATGATTACAGAC CAGGATTAAGGAGACGGAAACAAGGAGACAAAAAGACCTTCGATGAATGTGTGGCTGAGGGCGGCTCCGA CTGTGCTCCAGAGAAGCTCTGGCTTCAGATTCCGTTCTTCTGTGGCCACAGCTCAGAATGCTGGAATGTA GGGAGCAGATGGGCCATGGACCAGGCTAAGTCTAACCTCATTAATGAGTACTTCCTGGTGGGAGTCACCG AGGAGCTCGAGGACTTCATCATGCTGCTCGAGGCAGCTTTGCCCCGATTCTTCCGGGGCGCTACCGACCT CTACCGTACAGGAAAGAAATCTCACCTTAGGAAAACCACAGAGAAGAAGCTGCCCACCAAGCAAACTATC GCGAAGCTGCAGCAGTCTGACATTTGGAAGATGGAGAACGAGTTCTATGAGTTTGCTCTGGAGCAGTTCC AGTTCATCCGAGCTCACGCTGTCCACGAGAAAGACGGAGACCTCTACATCCTGGCCCAGAACTTTTTCTA CGAAAAGATTTACCCTAAGTCGAACTGAGCGCAGGTGTGGCCAGAGCACTCCTGCCCCGGAACTTGACTG TGTCACCACCCTTCTCAGCCCCCTGCTGATGCGTGTGCGAGCTGTGAGCCACGCTGAGCTGGGGACAGAC ATGAATGTCAAGGAGTGAGATGCTGGCGGGCTGGCGGAGTCCTGTGGAGTTTTTAACTTTCTGGCATTTT TACTTTTTTGTTGTTTTATTTTCGTTTTCATGATAGTTATAATCTTCTCCTGGTGCAGGGACATGGGGAC TTACATTGCTTAAAATACACACATGGACGTTTAGTCAGATGGCTGAATACCATTTCAGAAGAGGTTTGCA TAAGCATTTCTTTGCCTCTCCTTGAATGAAGAGGAGTCCGTTTGCCACTTACCCTTCTGGAGTTTGTGCC GCCACCTCTGCTGGATAGTGCTAAAATATGTTTGGCAGTATCTGATTTATTTGTATGAATCATGTCTCAC AAAGTTTATTAGAATGTTTGATCGTGTTTTCCCAGAACATTTCTGCTACAGTTGGGTTTGCCCATATTTA TGTAGGCTTTATTTTATTTGATTTGATGATCATTAGTGTTAAAGAAATCAACTTAAACCATGAATAATAC TGTAAGAAGGTAAAAAGTTAAAGCAGTATTCCTGACTCCTGTCACCCCAGTATCCAATATTGGAATGATA TTTCTAAGAATGTGGACATTATCTGAGGCTTTCTTAAGGATTTCCACATATCAATATTAAAAAATTGAGT TTAATACTTGTTCCTTCATGGCGTATCTGTACCCCTGAACACTGGCATATTGGTGACCGCACATGGCGAC TCCATCAGTGAGCTGTGATGTGGGTAGGATTTGCCTACTCTGTACTTTTACCTGTAGACTATTTTTACTA CGGTGCTTTATAATGTGTTTTAAAGCATTGCATTTACAAAAGGAAAGTGCTGTAAATATTGCATATTTTA TGTATTTGGACCAAAAAGTTACAAGTAAGTAGACAACAGTGGTGTTGCACCCGTTTTATTATGTCGAGTA AAGTCGTCAGACCTCCTATCCTATTCTGGTATTTCTCGTTTAACGTTACTGTTAGGATGTCACAAAAATG AGCTAACGTAGCCTTTCAAGTTTGATAGAGAACAGTGCTCACCCCTGAGGCAGTAGTGGTCATAGAAAAG GTCGTAGAGTCTGAGGGTTCCATCTCTGGTTCCAGCTCTGCTGTCACCTTCCTGCTGTGTGACGTTAGGA AGTCACCTGACCTCCCTGCCTCAGCTTCCTCATCTTGATATCAGGACACTAAGACCTTCTCTCCCTGCCT CACAGGGTTGTTGTGAGGACTAAGGAAGATGGCATGGCGAAGAGCACTTTAAAACTGTGGCGTGGTGTTC AAATGTGAGGTGTTGTCACAGAAGTATCTCTAGCTGATAGTTTCATAGTCTCCATTCTTTTAGCAACAAA AAGGGAAAATCCACTTTTAAGCTTTATGAGAAAAAAAAAACCCTTTCTCTTGATATAAATTTGTGTTTGA TAAATATCTTCTCAGTGTTTTATCTTCTGTGCTTAAGCAACTATTGAAGTGTGAACGGCTTATGCACTCG AGAGACTCAGACCGTCACTAGCTGAGGAGCAGGAAAGCCTTCTATAAACTTGAAGGATGTTCTTGTTTGT ATCTAACTTTGTCATCCGAAAATGGTGGCTATTTTACACTTGTGCTTGTTAAAGTGTCCTTTGTAGAATT TAATTTCTCTATTAATTCTTCACCTACTCCCTCTTTACAGCCAGGATTCGGATTCCTCTCAGACACACTT TTCTAAAGGAGTAATTATTTGTATCTTTAAATGTTTCTAAATAGGACCTCATCAAACAAAGCATTTTCAA CAGAAGACTTTAAAGTATTTATTGTGTAATAGGAGGAGTGAAGGACACTGTATATCAGAAAGCACTTAGG AAGATTAAAGGAACGACTGAGTCACAGGCTCCGCCGAGATGAAAGTATTAGGGGAACATAGCAACGTTTC CCTTCCCCCTCCGTCCTTTCCAGTGCAAGTTTCTAGGTCCTGCTAAAAGCAAGTTGTTTTCTTGTCCAGG CAGGCATCTGGCTGTTGAGTAAATCTGAGGTTATTCTTGAGAGTTCGCACTAAACTCTTAGGTAGTTAAG GCAGCACTTAGAACACTAACTGGGACTTTCCCTAAACTGTTCTCATAAAAGACCAGGTAATGAGTCAGGT CTCTGTGCAGCTCTTACTGTTGGAAAGTCGCTGTCACAGACAGGAGAGGCAAAGGGCCGTGGTTAGAGCT TAGCAAAACCTTTATTTACAGAACAGAAGGCATACTAGATATGAACCATGAGCCGCAAGCCCTCGCTCAC CCTGTGAGGGATTGTCATGGAGAAGCCCTGTCTGTGTCCGGACCACGTTCCCCAAAGAGTTCCTCTACCT CCCATGTCTCCCAGATCGTGACCCCAAGGACTGCTTTCTGACTCAGTCCCTCCCCAGCTTCCTTCTGTCT TACCTTGCTGTCAGGTGACTCGTTTTCCTAGCTCACCTGTACAGATTCGGGACATTTCTTTTATCGCTTT TTGGAGTGAATGGATTCCAAGTGTCCTTTCTGGAGACTAAGAAAAGGGTGGGCTTGCTTCTCTAAGAGTC CTGGGAGCCTGAGTCCTTAGAACATATAACTGTGTAATCAAATGAATACATGAGACAATGTGTATTTTTT ACATGTGGCAAATGCTTTTCTTTTTTTGGCCTTTGTGCTTTTTCAGTAATTTGACCTTAGAAAAAATGGT TTTTATATGATAGTAAAGCAAAATAACCACTTTGAATTTTAACGATAATGTCAATGTGTGTGTGTGTATC TATGTTTATTTCCTACAGAAGAAGAGGTAGCTTTGTGTGTTCAGCTTGTACCAACCGTCTTCTTATTGCT GAGAAATGGCCACTATTGATAAACCTTTCTGTTTTAAGATGTAGTGGGGAGGGTATGTTTTGATCTCCTC TGGCTTTAATTTCTAGAGTGTCACTTGAATATATCTGGTAGCAAACCTTGAAGCACCGCATAGGAGCTGC TTCTGTGTCCCCTGATAGTCACACAGTCCCTAACATCCGCTGCTTCTGGCCTCCTGAGAATTGGATTTAA ATATCCAAAACCCTCAAAACTCCAAGTTGCCAGCTTTTTAAACACTTGCTTTTCCCTTCTGTGCTGAAAA TCCAGATAGCACCTATAATTTGGACTCTATTGATCTTCCTCATTATGTGTCCTGTTGGAAACCCCTAAAA TTGTCTGCTCCATCAGGGAACTTACCAGAGTATTTTGATGTTGTATTTGCATAGATGGATATGAAATAGT TTATAGATGACAATTCTCTTTTTTGAATAAAATGAAATTTTTGAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_011828
• Insert Size: 1071 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC025443, AAH25443
• RefSeq Size: 4748 bp
• RefSeq ORF: 1071 bp
• Locus ID: 23908
• UniProt ID: Q8R3H7
• Cytogenetics: 3 H2
• Gene Summary: Catalyzes the transfer of sulfate to the C2-position of selected hexuronic acid residues within the maturing heparan sulfate (HS). 2-O-sulfation within HS, particularly of iduronate residues, is essential for HS to participate in a variety of high-affinity ligand-binding interactions and signaling processes. Required for metanephric development of kidney formation, suggesting that 2-O-sulfation within HS is essential for signaling between ureteric bud and metanephric mesenchyme. Mediates 2-O-sulfation of both L-iduronyl and D-glucuronyl residues.[UniProtKB/Swiss-Prot Function]