Kcnq1 (NM_008434) Mouse Untagged Clone

CAT#: MC205473

Kcnq1 (untagged) - Mouse potassium voltage-gated channel, subfamily Q, member 1 (Kcnq1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kcnq1
• Synonyms: AW559127; Kcna9; KVLQT1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC055304
  GCTCTGCGCTGCCTTCACCTCAGCTCCGAGGAGCAGCGGGGTCAGGGGTCCTGTCTGGCCATGGACACGG CCTCGTCCCCGCCCAGTGCTGAAAGGAAGCGCGCGGGTTGGAGCCGCCTGCTAGGCGCCCGGCGGGGCAG CGCGGTGGTCAAGAAGTGTCCCTTCTCACTGGAGCTGGCCGAAGGTGGCCCTGAGGGCAGCACGGTCTAT GCGCCCATCGCGCCAACCGGAGCCCCCGGGCTCGCGCCCCCCATGTCGACCCCAGTGTCGCCCGCCCCGG CCCCTGCAGACCTCGGCCCACGTCCGCGGGTGAGCCTTGACCCGCGGGTCTCCATCTACAGTGCGCGCCG CCCGCTGCTGGCGCGCACCCACATCCAGGGCCGAGTCTACAACTTCCTCGAGCGCCCCACGGGTTGGAAG TGTTTCGTGTACCACTTCACCGTCTTCCTCATTGTTCTGGTCTGCCTCATCTTCAGTGTCCTGTCCACTA TTGAGCAGTATGCCGCTCTGGCCACCGGGACCCTCTTCTGGATGGAGATTGTCCTTGTGGTGTTCTTTGG GACAGAATATGTGGTCCGCCTCTGGTCTGCAGGCTGCCGCAGCAAGTACGTGGGCATCTGGGGCCGGCTA CGTTTTGCCCGGAAGCCCATTTCCATCATTGACCTCATCGTGGTTGTAGCCTCTATGGTTGTCCTCTGCG TGGGTTCCAAAGGACAAGTGTTCGCCACATCAGCTATCAGGGGTATCCGCTTCCTTCAGATCCTGCGGAT GCTGCATGTCGATCGCCAGGGGGGTACCTGGAGGCTCCTGGGCTCTGTAGTCTTCATTCACCGCCAGGAG CTGATCACCACCCTGTACATTGGCTTTCTGGGCCTTATCTTCTCCTCCTACTTTGTCTACTTGGCTGAGA AAGATGCGGTGAACGAGTCCGGCCGCATCGAGTTTGGCAGCTACGCAGATGCTCTGTGGTGGGGGGTGGT CACAGTCACTACCATTGGCTACGGGGATAAGGTACCTCAGACGTGGGTTGGGAAGACCATCGCCTCCTGT TTCTCTGTCTTCGCCATATCCTTCTTTGCACTCCCAGCGGGGATACTTGGCTCTGGGTTCGCGCTGAAGG TCCAGCAGAAGCAGAGGCAGAAGCACTTCAACCGGCAGATCCCAGCTGCAGCCTCACTCATCCAGACTGC ATGGAGGTGCTATGCCGCTGAGAACCCTGACTCAGCCACTTGGAAGATCTATGTCCGGAAGCCTGCTCGG AGTCACACGCTTCTGTCCCCCAGCCCCAAACCTAAAAAGTCTGTCATGGTAAAGAAGAAGAAGTTCAAGC TGGATAAGGATAATGGGATGAGTCCTGGAGAGAAGATGTTCAATGTTCCTCACATCACTTATGATCCCCC AGAGGATAGGAGGCCAGACCATTTCTCCATTGATGGCTATGACAGCTCAGTAAGGAAGAGCCCTACACTG CTGGAAGTAAGCACACCCCATTTCTTGAGAACAAACAGCTTTGCAGAGGACCTGGACCTGGAAGGGGAGA CACTGCTGACCCCCATCACCCATGTGTCACAGCTGCGGGATCACCATCGGGCCACCATCAAGGTCATCAG GCGCATGCAGTACTTTGTAGCCAAGAAGAAATTCCAGCAAGCACGGAAGCCCTACGACGTGCGAGATGTC ATCGAGCAGTACTCCCAGGGCCACCTGAACCTTATGGTGCGCATTAAAGAACTACAGAGAAGGCTGGATC AGTCCATTGGGAAGCCATCTTTGTTCATCCCCATCTCAGAAAAGAGCAAAGACCGTGGCAGTAACACCAT CGGTGCCCGTCTGAACAGGGTGGAAGACAAGGTGACACAACTGGACCAGAGACTGGTGATCATCACAGAC ATGCTCCACCAGCTGCTGTCCATGCAACAAGGTGGTCCAACCTGCAACAGCAGGTCACAAGTTGTAGCCA GCAATGAAGGTGGCTCCATCAACCCTGAGCTCTTCCTACCCAGCAACAGCCTGCCCACCTACGAACAACT GACTGTGCCCCAGACAGGCCCTGATGAGGGTTCCTGAAGGAGCTCAGAAGGGAGGTCCAGGGTAAACCCC CCACAGGTCTGCCCCACCTCCCTCTCAACAGGGGCACACCCACCTGGTTCTCACCCCCTTGAAGGCCTGA TATGACAGCCTAGCTCCCTAAGGCCCCGAAACCCATGGGCCAAGCCACTGGCCTGGATCTGGATATCACC AACACCCCTCTGCCCAGCCAGTGGGAACTAGAACTTGTCTGGGCATGGGGTTCCTCTCAGGCCCCTATCA AGATGTTGATGCTGCTCTTCTGATGGCTGGAACATAGGGATGGGGAGTATATGTGTAAAGCAGGATGCAG CTCAGGCAGAGGGAAGCCCATCCAGGTATGCCAAGTGGCTTCCTAAAGGGAAACAGGAAGAGTCTGGGGT TTCCACCCTCAGCCCTGAATCCAGAGACCATCAGGGAACGTGCAGGGCAAGACAGGCCCGGCCCACACTA ACTGCAGGCTGTAGCCCAGTGGGAGCTCAGCAGGCTAGAGCCTGACTCCTTCATTCTCTAGTGATGTTGA CCACAACAAGATACTAGCGGGATTTTCACACCCCCCCCAAAGGATATCCCAAGCAAGGGATTGTCCACTG TCCACCCTAGCCAGGTATGGACTGACAACTTCTCCCCTAAAGTGAAAAGCAGGGCCCTCAAGCCCTATGA GCCCCACATGTGCAGCCTGCCTCCTTCTTGCTGGTCAGAGGTGCTGGACCAATGCTCAGACTCCAGCCTC TTTCACGCCACATTGAAGTTAGGGGCATCACCGATTCTTCCAGGATTGGTTCCTTGGGCTTGGGCACCAC GCCTCACAGAAATCAAGATAATTTCTGGTGATTCAGACTCTTGTGTTTTAATGAATTTCGTAGTATGATC TTGATTGAGAGACTTTTCCAAATAAAGTTGGGACGTCCCAGGTTAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_008434
• Insert Size: 2007 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC055304, AAH55304
• RefSeq Size: 3007 bp
• RefSeq ORF: 2007 bp
• Locus ID: 16535
• UniProt ID: P97414
• Cytogenetics: 7 88.12 cM
• Gene Summary: Potassium channel that plays an important role in a number of tissues, including heart, inner ear, stomach and colon (By similarity) (PubMed:16314573, PubMed:11120752, PubMed:15004216). Associates with KCNE beta subunits that modulates current kinetics (By similarity) (PubMed:17597584, PubMed:15004216). Induces a voltage-dependent by rapidly activating and slowly deactivating potassium-selective outward current (By similarity) (PubMed:8900282). Promotes also a delayed voltage activated potassium current showing outward rectification characteristic (By similarity). During beta-adrenergic receptor stimulation participates in cardiac repolarization by associating with KCNE1 to form the I(Ks) cardiac potassium current that increases the amplitude and slows down the activation kinetics of outward potassium current I(Ks) (By similarity) (PubMed:15004216, PubMed:17597584). Muscarinic agonist oxotremorine-M strongly suppresses KCNQ1/KCNE1 current (By similarity). When associated with KCNE3, forms the potassium channel that is important for cyclic AMP-stimulated intestinal secretion of chloride ions (By similarity). This interaction with KCNE3 is reduced by 17beta-estradiol, resulting in the reduction of currents (By similarity). During conditions of increased substrate load, maintains the driving force for proximal tubular and intestinal sodium ions absorption, gastric acid secretion, and cAMP-induced jejunal chloride ions secretion (PubMed:16314573). Allows the provision of potassium ions to the luminal membrane of the secretory canaliculus in the resting state as well as during stimulated acid secretion (PubMed:19491250). When associated with KCNE2, forms an heterooligomer complex leading to currents with an apparently instantaneous activation, a rapid deactivation process and a linear current-voltage relationship and decreases the amplitude of the outward current (By similarity). When associated with KCNE4, inhibits voltage-gated potassium channel activity (By similarity). When associated with KCNE5, this complex only conducts current upon strong and continued depolarization (By similarity). Also forms a heterotetramer with KCNQ5; has a voltage-gated potassium channel activity (By similarity). Binds with phosphatidylinositol 4,5-bisphosphate (By similarity).[UniProtKB/Swiss-Prot Function]