Mia2 (NM_146034) Mouse Untagged Clone

CAT#: MC205435

Ctage5 (untagged) - Mouse CTAGE family, member 5 (Ctage5), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mia2
• Synonyms: Ctage5; D12Bwg0579e; Mea6; Mgea; Mgea6
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC026864
  TCTTTCTCAAGGATCTGAAGTAGTCAGGAGAGCTTTAGTTACATGTCCGGGTGTTTTAACATGAATGTCC TAGGACATGGAATTGGAAGGTCTGGGTGAGGAGGTTGTGGCAGCATTGCCTGAAGATATGAGAGCAGACT TTAATCCCAGTGGTTTTTCGTTGGAATTGGCAGTATGTGTGCTTTCCGTTGGACTGCTTGCCGTGGTCTT GTTTCTGTGGCGAGGCTTCCGATCAATTCGAAGCCGATTTTATGTGGGGAGAGAAAAGAAACTTGCTCTT GAGCTTTCTGCACTAATTGAAGAAAAATGTAAACTGCTTGACAAAGTCAGCATTGTTCAAAAGGAGTATG AAGGCTTAGAGTCATCTTTAAAGGAGGCCAGCTTTGAGAAGGAGTCAACAGAAGCACAAAGTTTGGAGTT TGTTGAAGGATCACAAATATCAGAGGCCACCTATGAAAATCTGGAACAATCCAAATCTAAACTTGAAGAT GAAATACTACTCCTAGAGGAAAAGCTGGAAGAAGAGCGAGCTAAACATTCTGAACAAGACGAGCTGATGG CTGATATTTCAAAAAGGATACAGTCCCTAGAAGATGAATCAAAATCTCTCAAATCGCAAGTAGCTGAAGC CAAAACAACCTTTAGAATATTTGAAATCAATGAAGAGCGACTCAAGGGAGCAATAAAAGATGCCTTGAAT GAAAATTCTCAGCTTCAGGAAAGCCAGAAACAGCTTTTACAGGAAACTGAAATGATGAAAGAACAAGTGA ATGATCTCGATAAACAGAAAGTGGCATTGGAAGAGTCCAGAGCACAGGCGGAACAAGCTCTGAGTGAGAA GGAGAGTCAGATTGAGACTCTGGTCACGAGCCTGCTAAAGATGAAGGATTGGGCTGCTGTGCTTGGAGAA GACATAGCAGATGATGGGAATCTGGACTTGGACATGAAGAGTGGCCTAGAAAACACTGCTGCCTTAGATA ATCAGCCAAAGGGCGCTTTGAAGAAGCTGATTTATGCAGCTAAGTTAAATGCTTCTTTAAAAGCCTTGGA AGGAGAACGAAATCAAGTTTACACTCAGTTATCTGAAGTGGATCAAGTAAAAGAAGACCTTACAGAGCAT ATCAAAAGTCTTGAGTCTAAACAAGCATCTTTGCAGTCAGAAAAGACAGAGTTTGAAAGTGAGAGCCAGA AACTTCAGCAGAAACTGAAAGTGATAACCGAGCTGTACCAAGAAAATGAAATGAAACTTCACAGGAAATT AACAGTAGAAGAAAATTACCGATTAGAGAAAGAAGAAAAACTTTCCAAAGTAGATGAGAAAATCAGCCAT GCGACCGAGGAGCTGGAGACCTGCAGGCAGCGTGCCAAGGATCTTGAAGAAGAGCTGGAGAGAACAATTC ATTCTTACCAGGGGCAGGTTATTTCTCATGAAAAAAAAGCACATGATAATTGGCTGGCAGCTCGGACTCT TGAAAGAAATCTCAATGATTTAAGAAAAGAAAATGCCCACAACAGACAAAAATTAACTGAAACAGAGTTT AAATTTGAACTTTTAGAAAAAGATCCTTATGCACTTGATGTTCCAAACACAGCATTTGGCAGAGAGCACT CCCCATATGGTCCCTCACCATTGGGCCGACCTCCATCTGAAACGAGAGCTTTTCTCTCTCCTCCAACTTT GTTGGAGGGTCCACTAAGACTCTCACCTTTGCTTCCAGGGGGAGGAGGAAGAGGCTCACGAGGCCCTGAG AACCTTCTGGACCATCAGATGAACACTGAAAGAGGAGAGTCAAGCTATGACAGATTATCTGATGCTCCCA GAGCACCTTCCGACAGGTCCCTGTCACCTCCGTGGGAACAAGATCGAAGGATGACAGCCCATCCGCCACC AGGCCAACCATATTCTGATCCAGCTCTACAAAGGCAAGATCGGTTTTATCCTAATTCTGGTAGACTCTCT GGACCAGCAGAACTCAGAAGTTATAATATGCCCTCTTTGGATAAAGTGGATGGGCCAGTACCCTCAGAAA TGGAGTCCAGTGGAAACGGTACCAAAGATAATCTTGGTAACTCTAATGTGCCTGATTCTCCTATCCCTGC TGAATGCGAAGCAGCTGGCCGTGGCTTCTTCCCTCCACCCTTTCCTCCAGTCAGAGACCCATTGTTTCCA GTGGATCCAAGGAGCCAGTTCATGAGAAGGGGCCCCTCTTTCCCCCCACCTCCTCCAGGAAGCATCTATG CAGCTCCTCGAGATTATTTTCCACCAAGGGACTTCCCTGGCCCACCACTTCCTCCGTTCCCAGGGAGAAC TGTGTACGCACCGAGGGGCTTTCCACCTTACCTTCCCCCCAGAGCTGGGTTCTTCCCCCCACCCCCACAT CCTGAAAGTAGAAGTGAGCTCCCCCCAGACTTGATTCCGCCTTCAAAAGAGCCTGCAGCTGACCCTCCAG AAACACAGGAGGCCTGACAGCGTTACGTCTTACCGGCAGTCATTTCCGTCTCATTTTCAGTTTAAGTAAC TGCTGTTACTTATGTGATTATATTTTTGCTCAAATTGAAGCTTAATGGAATTATAATTCTCAGGATAGTA TTTTGTAAATAAAGATGATTTAAATATGAATCTTATGGGTAAATTATTTCCATTTTATTTTATTCTAGAT AGTATAACTATTTTACTTTGATTACCTCATCTATAATTATGGTAGTTTTATATATGTAATCTTATAGTTG GGGATATCTCAAACTCAAAGGCTGTGTCTTTATACCAGGGATGTGTTTACCATGGTTGTAGGCAATGTAA AAGTAACTTCATGTTTAATCAAATAAAATTTTAATTGATTTAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_146034
• Insert Size: 2340 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC026864, AAH26864
• RefSeq Size: 2856 bp
• RefSeq ORF: 2340 bp
• Locus ID: 338320
• Cytogenetics: 12 C1
• Gene Summary: This gene encodes a protein that is involved in endoplasmic reticulum-to-Golgi trafficking and regulation of cholesterol metabolism. Three major classes of transcripts are generated from this gene- melanoma inhibitory activity 2-specific transcripts, cTAGE family member 5-specific transcripts and transcripts that include exons from both these transcript species. Additionally, alternative splicing in these transcripts results in multiple transcript variants encoding diverse isoforms. A mutation in this gene (couch-potato or cpto) may result in low levels of plasma cholesterol and triglycerides. [provided by RefSeq, Sep 2016]
  Transcript Variant: This variant (3) lacks several 5' exons and reflects the use of an alternate internal promoter, compared to variant 1. It encodes the cTAGE family member 5 protein (isoform 3), which is shorter and has a distinct N-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. CCDS Note: The coding region has been updated to extend the N-terminus to one that is supported by available conservation data. The use of an alternative upstream start codon would result in a protein that is 17 aa longer.