Gfm2 (NM_177266) Mouse Untagged Clone

CAT#: MC205410

Gfm2 (untagged) - Mouse G elongation factor, mitochondrial 2 (Gfm2), nuclear gene encoding mitochondrial protein, transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gfm2
• Synonyms: 6530419G12Rik; A930009M04Rik; EF-G2mt; EFG2; MST027; RRF2mt
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC027341
  CGGACGCGTGGGCGGACGCGTGGGCGGACGCGTGGGCGGACGCGTGGGCGGACGCGTGGGTCCGCGGGTT CCTTCTGCGGAGAACGTTTTAGAGGACCACGATGTTCACCAAATGGAGGATTTTTGCAGTGAATCATCAG AGAACTTTCAGTGTGCACCTTAATACCATGTGTTACTGCAAAATAAAAGCAAATTTAAAAAGATTAAAGA CTCAGCTGCCTCTTACAAGAAACTACAGTTCTGCACCAGCAGGAAGTGATGTCAAGTCTCTTCATTCCGT CATTAACCCTCCTGTGGCCAAAATTCGTAATATTGGAATTATGGCACATATTGATGCAGGCAAAACCACC ACCACAGAGAGAATTCTGTACTATTCTGGATACACAAGATCATTGGGAGATGTCGATGATGGAGACACAG TGACGGATTTCATGGCTCAAGAACGAGAAAGGGGCATTACCATCCAGTCAGCAGCTGTGACTCTTGACTG GAAAGGGTATAGAGTCAATCTGATTGATACTCCAGGTCACGTAGACTTCACTCTAGAGGTCGAGCGCTGC TTGAGGGTGTTGGACGGTGCGGTGGCCGTGTTTGACGCCTCTGCTGGTGTAGAGGCCCAGACTCTCACTG TATGGAGGCAAGCTGACAAACACAAAATACCCCGGATCTGCTTTTTAAACAAGATGGACAAGACTGGAGC AAGTTTTAACTATGCAGTTGAAAGCATCCGGGAGAAGTTAAAGGCAAAGCCCCTGATTTTACAGCTGCCA ATTGGTGAAGCCAGAACTTTCCAAGGAGTGGTGGATGTCGTAAACAAAGAAAAACTCGTTTGGAATTCTA ACTCTGACGATGGAAAAGACTTCGAGAGGATGCCCCTCTCAGAAGCGAGTGATCGGGAACTGCTAAAGGA GACGATTGAAGCAAGGAACTCCTTAATTGAACAAGTTGCAGACTTAGATGATGAGTTTGCTGACTTGGTT TTAGGTGAATTTAGTGAAAACTTTGATTTAGTACCAGCTGAAAAGTTACAGGCTGCAGTGCACAGGGTGA CACTGGCACAGGCTGCAGTGCCTGTGCTGTGTGGGAGTGCCTTGAAGAACAAGGGCGTGCAGCCCCTGCT GGATGCGGTGACCACTTACCTGCCGTCACCCGAGGAGCGCGAGGACCGTTTTCTGCAGTGGTATGAGGGT GACTTGTGTGCACTGGCGTTTAAAGTACTGCATGACAAACAGCGAGGACCCCTGGTTTTCCTGCGCATTT ACTCGGGAACACTGACGCCTCAGCTGGCTGTCCACAACATTAATAGAAACTGCACGGAAAGAATGAGTCG TCTGCTTTTGCCATTTGCTGACCAACATGTAGAAATTCCTTCCCTGACTGCTGGAAACATTGCTTTGACA GTTGGGCTTAAACAAACTGCCACTGGAGACACCATAGTCTCGTCCAAGTCCAGCGCATTAGCTGCCGCTC GCAGAGCCGGACGAGGAGAAAGGGAGCATGGAAAGAAGAGAGAGGCCGAGAGTCTGCTGTTGGCCGGGGT GGAGGTCCCAGAGCCTGTCTTCTTCTGCACCATAGAGCCCCCGTCAGTCGCTAAGCAGCCAGATTTGGAT CATGCCTTGGAACGCCTTCAGCGCGAAGATCCCAGTTTGAAAGTGAAACTCGATCCTGACTCTGGACAAA CTGTCCTGTGTGGAATGGGGGAGTTGCATATTGAGATTATTCATGACCGTATCAAGAGGGAGTATGGCCT AGAGACCTATCTAGGGCCTCTGCAGGTGGCATACCGGGAAACCATCTTAAACTCAGTTCGCGCCACAGAC ACCCTCGATAGGGTGCTTGGAGACAAGCGGCACTTGGTGAGTGCGGAGCTGGAAGTCCGGCCAGCAGAAG AGCCCTGTGCTGTGGCCAAGATCGAGTATGCTGACTGTGTGGGTGAAGACCTGCTGCAGGCCTCTCGGGA GGCCATTGAAAGTGCCGTCCACAGTGCATGCCTCCAGGGACCTCTGCTCGGATCCCCAGTCCAGGACGTG GCAATGACGTTGCACTCACTGATGATTCACCCTGGCACCTCCACAACTATGGTCACTGCCTGCATCTCAC GGTGTATGCAGAAGGCCCTGAAGAAAGCTGACACACAGGTGCTGGAACCTCTGATGAGTCTCGAGGTCAC AGTGAGCAGGGAGTACCTCAGCCCTGTCCTGGCAGACTTGGCACAAAGAAGAGGGAACATTCAGGAAATC CAGACTCGCCAAGACAACAGAGTTGTTCTAGGATTCGTTCCTCTAGCAGAGATTATGGGATATTCAACTG TGCTTCGAACTCTGACATCTGGCTCAGCTACTTTTGCCTTAGAACTGTCTACTTATCAAGCCATGAGTCC TCAAGACCAGAGTGCTCTACTCAACCAGAGAAGTGGCTTGGCCCGTGTGCTATAGGCCCTTGGAAACGGG GAAAGCTGGGCCAGTTGCCTGCTTTATTCCCGGCAAGAATGCTGTTTTATTAACTGGCTAGTAAATTGCT GCTTTGGTGTGGAAAGAACTTGACAAGACCCTTGGAGCTGCCATTCTAATTCTGTTGGGTGTACTTTGCT ATATTGCACCACAGCTGGGCCCCGGTTATCTCTGCCATCTCTGGTGCTGGGATAAAATAACCTGGCCAGG GCAGCTTAGGAAAAAAAAAAAAGTTTCTTTTCTCAGCAGGAAGCAAGGCAATGAGCTCTCAAAGTCCACC TAGTGATGAACTTCCTGAAGCCAGGATACACTGCCTAAGCCTCCCCAAACAGCACCACCCCCGGGAGCAG AGTCCAGAAGCCTGGGCCTGTGAGGAGCGGACCTCGTTTGGACATTTCTTGTTTTACTTTTGAGATGTTC CATCCCCCTGGAGCTGGAATTGTGGTGTGATTACGGAGATCCAGGGCCTGAGCTCGGGTCCTCTGCAAGA GTGATCTTAAATACCTCAGCCTGCTTTCTAGCCTGACATCTCTCTTTTAAACCATTGCAAGATGACAGTA TCCACACAGGGCAGCCCTCTGGAGTGCTAGTGCAAGGCTTTTCCTTTTATTTTCTTAAGCTGCTCACATA AAAGATTTTTTTTTTGTTTGTTTGTTTGATTCAGGGATAGAAAGATTCAATCAATAAAAAGATGGTTTAT TTAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_177266
• Insert Size: 2334 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC027341, AAH27341
• RefSeq Size: 3177 bp
• RefSeq ORF: 2334 bp
• Locus ID: 320806
• UniProt ID: Q8R2Q4
• Cytogenetics: 13 D1
• Gene Summary: Mitochondrial GTPase that mediates the disassembly of ribosomes from messenger RNA at the termination of mitochondrial protein biosynthesis. Acts in collaboration with MRRF. GTP hydrolysis follows the ribosome disassembly and probably occurs on the ribosome large subunit. Not involved in the GTP-dependent ribosomal translocation step during translation elongation.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longest isoform (1). Variants 1 and 3 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.