Ddx25 (NM_013932) Mouse Untagged Clone

CAT#: MC205011

Ddx25 (untagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 25 (Ddx25), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ddx25
• Synonyms: AW047046; GRTH
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC061130
  AGGGGCGAGAGCGGAGACCGCAGCTATGGCGTCGTTACTTTGGGGAGGCGACGCCGGGGCGGCGGAGAGC GAGCGGCTGAACAGCCACTTTTCAAATCTCGTCCATCCCAGGAAGAACCTTCGTGGTATTAGGAGTACCA CAGTCCCAAACATAGACGGCTCTCTTAACACGGAAGATGATGATGATGATGAAGACGATGTAGTGGATTT GGCAGCCAATTCACTCTTAAACAAGCTAATCCGTCAGTCCTTAATAGAATCCAGCCACCGAGTGGAAGTC TTACAGAAGGATCCCAGCTCTCCACTCTACTCGGTGAAAACCTTTGAAGAGCTACGGCTAAAAGAAGAAT TACTAAAAGGGATTTATGCAATGGGATTTAACCGACCATCCAAAATCCAAGAGATGGCCCTCCCCATGAT GCTAGCACACCCTCCCCAGAACCTCATAGCACAGAGCCAGTCCGGCACAGGAAAAACTGCTGCCTTTGTC TTAGCAATGCTAAGCCGAGTGAATGCCTTGGAACTGTTTCCACAGTGCCTCTGCCTGGCTCCTACCTATG AACTGGCTCTGCAGACCGGTCGTGTGGTTGAAAGGATGGGCAAGTTCTGCGTGGATGTTGAAGTGATGTA TGCCATTCGAGGAAACCGAATTCCAAGGGGCACCGAAGTCACCAAACAGATTATTATTGGTACTCCTGGG ACTGTCCTAGATTGGTGTTTCAAGCGAAAACTGATTGATTTGACAAAGATCCGTGTGTTTGTCCTGGATG AAGCAGATGTGATGATTGACACCCAAGGATTCTCCGATCAGAGCATCCGTATCCAAAGAGCTTTACCCTC TGAATGCCAGATGCTCCTCTTTTCGGCAACCTTTGAGGACTCTGTGTGGCAGTTTGCTGAACGAATCATT CCTGACCCCAATGTTATCAAGTTGCGAAAAGAGGAACTGACCCTGAACAACATCCGGCAGTATTACGTGT TGTGTGAGAACAGGAAAGGCAAATACCAAGCCCTCTGTAACATCTACGGTGGCATCACCATTGGCCAAGC CATTATCTTCTGCCAGACACGTCGAAATGCCAAGTGGCTGACTGTGGAGATGATGCAGGATGGCCACCAG GTGTCCCTGCTAAGTGGGGAACTCACAGTGGAGCAGCGAGCTTCCATCATTCAGAGATTCCGAGATGGCA AAGAGAAGGTTCTCATAACGACCAATGTCTGTGCTCGAGGGATTGATGTGAAGCAGGTCACCATCGTCGT GAACTTTGACCTCCCTGTAAACCAATCAGAAGAGCCAGACTATGAGACCTACCTCCACCGTATAGGGCGG ACGGGACGCTTTGGGAAAAAAGGTCTCGCCTTCAACATGATTGAAGTGGATAAGCTGCCCCTGCTTATGA AAATACAAGACCACTTCAATAGCAATATTAAGCAGCTTGACCCAGAAGACATGGATGAGATTGAGAAGAT CGAATATTGAAGAGAGAGAGAGAAGAGAGGCCTTCGTTGTTTGTGGGCTAGTTTACGTGAGAATGTTTCA GCAGAGTTTGAAGATAATTTTCTATGTTGAGACTCTGTCAAGGTGAAACTGTCACAGATAAAAAATAAAT GCCATGACTCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: SfiI-SfiI
• ACCN: NM_013932
• Insert Size: 1110 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC061130, AAH61130
• RefSeq Size: 1652 bp
• RefSeq ORF: 1110 bp
• Locus ID: 30959
• UniProt ID: Q9QY15
• Cytogenetics: 9 A4
• Gene Summary: ATP-dependent RNA helicase. Required for mRNA export and translation regulation during spermatid development.[UniProtKB/Swiss-Prot Function]