Gkap1 (NM_019832) Mouse Untagged Clone

CAT#: MC204946

Gkap1 (untagged) - Mouse G kinase anchoring protein 1 (Gkap1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gkap1
• Synonyms: 42kDa; 4933400B15Rik; D13Ertd340e; Gkap42
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC052347
  GCCGCCCTCCGCGGGCCCGTTACTGCGGACGCCCCGCGGCCCGCGCCCAACGCCCGGGGGTAGCGCGGGA GCGGCGGGGAGACGGGAGCGGCCGCACCGCGGCGAGGGAGGCCCGGAGTTACTATTGAGCAAGCGTTTAA GCCCCCAGTTCTGAGCCATGGCCTCTGCAGTCCTCAGCTCTGTGCTCACAACTGCCTCTCGCTTTGCCCT GCTACAAGTGGACAGCGGCAGTGGCTCTGATTCCGAGCCTGGGAAAGGCAAAGGCCGGAGTAATGGGAAG TCTCAAACTCTGGGAAACAAAGCAACCTCAAATGAGAAGAAACGGGAGAAGAGAAGAAAAAAGAAGGAAC AGCAGCAGAGTGAAGCAAACGAGCTCAGGAATCTTGCTTTCAAGAAAATTCCCCAGAAATCCTCCCATTC CATTTGCAATGTTCAACATGAGCTTTCATCGCCAAACCCAGCACAGAAGGAGTCACGGGAAGAAAACTGG CAAGAGTGGAGACAGAAAGATGAACAGCTGACCTCTGAAATGTTTGAAGCTGACCTTGAGAAGGCGTTGC TGTTGAGTAAGCTGGAGTATGAAGAGCACAAACAGGATTATGAAAATGCTGAGACTGCTTCAACTCAGAC AAAAGGTATAAATAAAAAAGATAAAAGGAAGAACCACCAGGGCAAGGACAAACCTGTCACGGTGTCACTC AAAGACTTCCAGTGTGAAGATCATATTAGTAAAAAGGCAGAGGAATCGAATTCTGCTCAGACTTTATCAC ACGATGGGGGATTCTTCAATAGACTGGAAGATGATGTTCATAAGATTCTGATTAGAGAAAAAAGGAGAGA ACAACTCACAGAGCACAACGGAACAGATAACTCTCCCGCTCCAGAGCACAACCAGGAAGTAGGTCTAAAA GATGGAAGAATTGAAAGACTAAAGTTAGAACTTGAAAGGAAAGACGCAGAAATTCAGAAGCTCAAAGCTG TGATCACTCAGTGGGAGGCAAAGTATAAAGAAGTAAAAGCAAGAAATGGACAATTACTGAAAATGCTTCA GGAGGGAGAGATGAAAGATAAGGCCGAGATACTTCTGCAAGTGGATGAGTCTCAGAGCATCAAGAACGAG CTGACGGTCCAGGTGTCTTCACTTCATGCCGCACTGGAACAAGAAAGATCTAAAGTGAAAGTATTACAGG CAGAATTAGCCAAATACCAGGGAGGCAGGAAGGGGAAAAGGAACTTCGAACCTGACCATTGCAGGTGATC GTGGCCTCGGAGGCCAGCACAGAATTCTTTCACACTTTTGCAGAAATGTGTATATTTAATGCTGTGCGAC TGCTAAACTGCAGGTTTTTGTTGAAGGAACTAAAAGCAACTAGTTCACTCATAGTCTACAGTTTTATGTT CTTTTGGCTTAAAGTTAAAAGAAAATACAGAATACCAGCAGAACTTGATTATTGTTTTCTGTCCAAACTT CTTATGACTTTAGTTTTTCATCAGTCAATAAAATTAATTTACTCTTCCGAAAAAAAAAAAAAAAAAAAAA AAAAAAAA
• Restriction Sites: SfiI-SfiI
• ACCN: NM_019832
• Insert Size: 1101 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC052347, AAH52347
• RefSeq Size: 1548 bp
• RefSeq ORF: 1101 bp
• Locus ID: 56278
• UniProt ID: Q9JMB0
• Cytogenetics: 13 30.97 cM
• Gene Summary: Regulates insulin-dependent IRS1 tyrosine phosphorylation in adipocytes by modulating the availability of IRS1 to IR tyrosine kinase. Its association with IRS1 is required for insulin-induced translocation of SLC2A4 to the cell membrane. Involved in TNF-induced impairment of insulin-dependent IRS1 tyrosine phosphorylation.[UniProtKB/Swiss-Prot Function]