Kat2a (NM_020004) Mouse Untagged Clone

CAT#: MC204844

Kat2a (untagged) - Mouse K(lysine) acetyltransferase 2A (Kat2a), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kat2a
• Synonyms: 1110051E14Rik; AW212720; Gcn5; Gcn5l2; mmGCN5
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC063752
  GCGGCCCTAGGGCTGGGAGCGCGGCGCCGCTCCGCTGCGGGGGAGGCCATGGCGGAACCTTCCCAGGCCC CAAACCCGGTCCCGGCAGCGCAGCCCCGGCCCCTTCACTCCCCAGCCCCTGCCCCAACTTCGACTCCTGC GCCCAGTCCGGCTTCAGCCTCCACTCCGGCTCCGACTCCGGCACCAGCCCCTGCCCCAGCTGCAGCCCCA GCTGGGAGCACAGGGAGTGGGGGCGCCGGGGTAGGAAGTGGGGGGGATCCGGCTCGACCTGGCCTGAGCC AGCAGCAGCGCGCTAGCCAGAGGAAGGCGCAAGTCCGGGGTCTGCCGCGTGCCAAGAAGCTTGAGAAACT AGGGGTCTTCTCAGCTTGCAAGGCCAATGAAACCTGCAAGTGTAATGGCTGGAAAAACCCCAAGCCCCCC ACCGCACCCCGCATGGACCTCCAGCAGCCAGCTGCCAACCTGAGCGAGTTGTGCCGTAGCTGTGAGCACC CTTTGGCGGACCATGTGTCCCACCTGGAGAATGTGTCAGAGGACGAGATTAACCGGCTCTTGGGAATGGT AGTAGATGTAGAAAATCTGTTCATGTCTGTTCACAAGGAAGAGGACACAGACACCAAACAAGTCTATTTC TACCTCTTCAAGCTCTTGCGGAAGTGCATTCTGCAGATGACACGGCCAGTGGTGGAGGGGTCTCTAGGCA GCCCCCCATTTGAGAAGCCTAACATTGAACAGGGTGTACTGAACTTTGTGCAGTACAAATTTAGCCACCT GGCCCCCCGAGAGCGCCAGACAATGTTTGAGCTCTCAAAGATGTTCCTGCTCTGCCTTAACTACTGGAAG CTGGAGACCCCTGCTCAATTCCGGCAGCGGTCCCAGTCTGAGGATGTCGCTACCTACAAAGTCAATTATA CCAGATGGCTCTGTTACTGCCACGTGCCCCAGAGCTGCGACAGCCTCCCCCGCTACGAGACCACCCACGT GTTTGGCCGAAGCCTTCTACGGTCCATTTTCACTGTCACCCGCCGGCAGCTACTGGAAAAATTTCGGGTG GAAAAGGACAAACTGGTGCCTGAGAAGAGGACCCTCATCCTCACTCACTTCCCCAAATTCCTGTCCATGC TTGAGGAAGAGATCTACGGGGCAAATTCTCCCATCTGGGAGTCAGGCTTCACCATGCCACCTTCGGAGGG GACACAGCTGGTGCCCCGGCCAGCCACGGTCAGTGCAACAGTTGTACCCAGCTTCAGTCCTTCCATGGGA GGAGGCAGCAACAGCTCCCTGAGCCTAGATTCCGCAGGGACTGAGCCCATGCCAGGGGAGAAGAGGAAGC TTCCTGAGAACCTGACCCTGGAGGATGCCAAGCGGCTCCGCGTGATGGGGGACATCCCCATGGAGCTGGT CAATGAGGTCATGCTGACGATCACTGACCCCGCTGCCATGCTGGGGCCTGAGACAAGCCTGCTTTCGGCC AACGCAGCTCGAGATGAGACGGCTCGCCTGGAAGAACGGCGAGGAATCATCGAGTTCCACGTCATTGGCA ACTCCCTGACGCCCAAGGCCAACCGGCGCGTGCTGCTCTGGCTCGTGGGGCTGCAGAATGTCTTTTCCCA CCAGCTGCCAAGAATGCCCAAGGAATATATCGCCCGCCTCGTCTTTGACCCGAAGCACAAGACCCTGGCC CTGATCAAGGATGGGCGGGTCATCGGTGGCATTTGCTTCCGCATGTTTCCCACTCAGGGCTTCACGGAAA TCGTCTTCTGTGCCGTCACCTCAAATGAGCAGGTCAAGGGCTATGGCACCCACCTGATGAACCACCTAAA GGAGTACCACATCAAACACAGCATCCTTTACTTTCTCACCTATGCTGACGAGTACGCCATTGGCTACTTC AAAAAGCAGGGCTTCTCCAAAGACATCAAGGTGCCCAAGAGCCGCTATCTGGGCTACATCAAGGACTATG AAGGAGCGACGCTGATGGAGTGTGAGCTGAATCCCCGAATCCCCTACACAGAGCTGTCCCACATCATCAA GAAACAGAAAGAGATCATCAAGAAGTTGATTGAGCGCAAACAGGCACAGATCCGCAAGGTCTACCCTGGA CTCAGCTGCTTCAAAGAGGGAGTGAGGCAGATCCCGGTGGAGAGCGTCCCTGGCATTCGAGAGACAGGCT GGAAGCCACTGGGAAAGGAGAAGGGCAAGGAGCTTAAGGATCCTGATCAGCTATATACGACCCTCAAAAA CCTGCTGGCTCAGATCAAGTCACACCCCAGTGCCTGGCCCTTCATGGAACCTGTGAAGAAGTCAGAGGCT CCTGACTACTACGAGGTTATCCGTTTCCCCATTGACCTGAAGACCATGACAGAACGGTTGCGCAGTCGCT ACTATGTGACCCGGAAGCTCTTCGTAGCTGACCTGCAGCGGGTCATCGCCAACTGCCGGGAGTACAATCC CCCAGACAGCGAATACTGTCGCTGCGCCAGTGCCCTGGAGAAGTTCTTCTACTTCAAGCTCAAAGAGGGA GGGCTCATCGACAAGTAGCCCCCAGCTGGGATCCGCAACATCCACCTCCATCTCCTCAGATCCGTTTGGG GGCTCCCGGCCCCCTCTCCCGGACTCAGCTGGAGAAACTCCAGCCAGGGACCCCAGATCTTACAGACTTT CTGGCCAGCAGGCACTCACTCCCAAGCCAGCAGCTCTCTTTCCACCTTCCTTGCATCCCACCTTGGGGAG ACGTCTGGTCTGGGGCCAGCCCGTTGTAGAATGTCTGGTGGCCAGGGCATGAACTGTTGGTCCTTATAGA GCCATGCAGGCTGAGCATCCTGAGCCTGGTCCCCATGCCTGTTTCCCAGGGCCAGGACTATTCAGTGAGT AGCCTTCACATGGAAACAGCCCAGACTGGAGGAACCATGAGGGGCCTTGGCTTATCCTACACTGTTGTCT ATCTGTCCCTCCAGGATTGGGGGCAGGAGGACCATTCATTCCTTGGCATTAATCCCTCGGAGGGAACAAT AAAGCTTTTTTTTTCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_020004
• Insert Size: 2490 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC063752, AAH63752
• RefSeq Size: 3095 bp
• RefSeq ORF: 2490 bp
• Locus ID: 14534
• UniProt ID: Q9JHD2
• Cytogenetics: 11 63.53 cM
• Gene Summary: Protein lysine acyltransferase that can act both as a acetyltransferase and succinyltransferase, depending on the context (PubMed:28424240). Acts as a histone lysine succinyltransferase: catalyzes succinylation of histone H3 on 'Lys-79' (H3K79succ), with a maximum frequency around the transcription start sites of genes (By similarity). Succinylation of histones gives a specific tag for epigenetic transcription activation (By similarity). Association with the 2-oxoglutarate dehydrogenase complex, which provides succinyl-CoA, is required for histone succinylation (By similarity). In different complexes, functions either as an acetyltransferase (HAT) or as a succinyltransferase: in the SAGA and ATAC complexes, acts as a histone acetyltransferase (By similarity). Has significant histone acetyltransferase activity with core histones, but not with nucleosome core particles (By similarity). Acetylation of histones gives a specific tag for epigenetic transcription activation (PubMed:28424240). Involved in long-term memory consolidation and synaptic plasticity: acts by promoting expression of a hippocampal gene expression network linked to neuroactive receptor signaling (PubMed:25024434). Acts as a positive regulator of T-cell activation: upon TCR stimulation, recruited to the IL2 promoter following interaction with NFATC2 and catalyzes acetylation of histone H3 at Lys-9 (H3K9ac), leading to promote IL2 expression (PubMed:28424240). Also acetylates non-histone proteins, such as CEBPB, PLK4 and TBX5 (PubMed:17301242). Involved in heart and limb development by mediating acetylation of TBX5, acetylation regulating nucleocytoplasmic shuttling of TBX5 (By similarity). Acts as a negative regulator of centrosome amplification by mediating acetylation of PLK4 (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).