Gprc5b (NM_022420) Mouse Untagged Clone

CAT#: MC204568

Gprc5b (untagged) - Mouse G protein-coupled receptor, family C, group 5, member B (Gprc5b), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gprc5b
• Synonyms: AW125761; Raig2
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC020004
  CGGACGCGTGGGGCGGGCTCGGCTGGCGGGCGTGCGCGGAGCGGGGGCCGCGGGCGCGCTGCAGAGATGT GACTTGGGCCCAGGGCCAGCAGGAGCGTCGGCGCTGCGGGGACGCGAGGGTCGAATGTTCCTGGTGTTAG AGAGAAAGATGAGAACCCATCAAGTGTTTCCCTTGCCCCTGCTCCTGGTGATTGCCTCCGTGGCTTCAGA GAACGCCAGCACGTCCCGGGGCTGTGGACTGGACCTTCTTCCTCAGTACGTGTCCCTGTGCGACCTGGAC GCCATCTGGGGCATCGTGGTGGAGGCAGTGGCCGGGGCGGGGGCCCTGATCACACTGCTTCTGATGCTCA TTCTCCTAGTGAGACTACCCTTCATCAAGGACAAGGAAAGGAAGCGGCCTGTGTGCCTCCATTTCCTCTT CCTGCTGGGGACCCTGGGCCTCTTTGGCCTGACGTTTGCCTTCATCATCCAGATGGACGAGACAATCTGC TCCATCCGACGCTTCCTCTGGGGTGTCCTCTTCGCGCTCTGCTTTTCCTGCCTGCTGAGCCAGGCGTGGC GGGTGCGGAGGCTGGTGCGCCAGGGCACAAGCCCGGCCAGCTGGCAGCTGGTGAGCCTGGCACTGTGCCT GATGCTGGTGCAGGTCATCATTGCCACTGAGTGGCTGGTGCTGACTGTGCTGCGTGACACGAAGCCAGCC TGCGCCTACGAGCCCATGGATTTTGTGATGGCGCTCATCTACGACATGGTGCTGCTGGCCATCACCCTGG CCCAGTCCCTCTTCACGCTGTGTGGCAAGTTCAAACGGTGGAAGGTGAACGGAGCCTTCATCCTCGTCAC TACCTTCCTCTCTGCGCTCATCTGGGTGGTCTGGATGACCATGTACCTCTTCGGCAACTCATTAATTAAG CAGGGAGATGCCTGGAGCGACCCCACCTTGGCCATTACACTGGCAGCCAGCGGATGGGTCTTTGTCATCT TCCATGCCATTCCGGAGATCCACTACACCCTTCTCCCACCCCTGCAGGAGAACCCACCCAACTACTTCGA CACTTCACAGCCCAGGATGCGGGAGACAGCATTTGATGAGGAAATGCACCTGCCTCGGGCCTACATGGAG AACAAGGCCTTCTCAATGGATGAACATAACGCAGCTCTCCGATCAGCAGTGGGCTTTTCCAATGGAAGCT TGGAGCAAAGATCTAGCAGCCTGGGAAAGAAGCCCAGCAGCTTGGGAAACCGGCCCAGTGCACCGTTCAG AAGCAATGTGTATCAGCCGACCGAGATGGCCGTCGTGCTCAATGGAGGGACTATCCCAACTGCTCCGCCA TCTCACACGGGAAGACACCATTGGTGAAAGACCTTAAGTTCCAGAGAATAAGAATATTTCTTACCGATTT TATTCCCTGGCTGTGTCTTTCTTGAGGGCGAAATCACTAACCATGGCAGAGACAGGCCACCCGGAAGCCA GGAGATCTGAGAAGTCCCACCAAGGGGATTTCGTGTAAATGTGACATATGGATGAACTGGAAAGCTAACA GTGACCGCCCTCCCCTCCCCTGCCACACATGCAAACACGGAATCGTCCTGCAAACTAAATCAGAGCTAAC TGCCAATAGTCTTAGACTCACTCAGAAGCGTGGCTGGGAAACTGAGTCATTCAGCAGGGGGCAGGACAGA GAAGAAGTCACAGCTGCTGTACCAGACTATCTTGGCTTATTATGTGAGGGGTTGGAGCCAGCCTGTCACC CCCACCCTCCTCACCAAATGCTGGTCCCCAGAGCCATCTTTTGGAGAGAACTGTGCCCTTGAGGTTAACT CATGGATCATTCTCCCTGGCTTTTGCTTGCCTGGTGGCCCATGCAGTTCAGGAGACTTTAAGAGGTTGTG GCTATGATTCTCAAGGCCCAGCTGGATGGTCAGGGAGCTGTGTAGCCAGTAGGAATGGAGGAACCATAGA ACGTGCCAAAGAAGAGAAGGTACTCTGGACTTTGAAGTGACCATCACATGTAGACAGAGGTGACCAACCG TTGTTCCTTCTGTGGGCCTCTTGCTCTACCAAGCACCCCACCTACCCCAACCTCCCCTACAGAGTGATAG AAATATCCCTCCTCCTTCCTCTTGCAGGTGCTCCTCCATGCCTTCCCCCAACCCTAGCCCACCCTCACCC TCCACCCCCTAACCCCCAAGTTCATTACCATCTTCATTTCAGAAGCTCAAGTAGGGTCGCCAGCTTGAAG CTTCAGGGGAGGCAGGCTGAAAGGCAGGCGCCATGGCGCCTTTTCGTAATGATGTCCTATGGCTGGGTTC CCCAGGGGTGACCTCTAGGATCGAGAGATGGCATCCTGTTGCCCAGCTGCCAGTGGCTTCTGGGATATTT CCCCTTCATGTGGGTAGAAGTCCAGGATACAATGAGCTCCATGAAGGTTTCTGGCTGCTTTTGGAGGGTA TGGAGAACGTCTTGATTCTGGTTTTCCGATGGCTCTATGGATGTGGCCCCCTTCACAACTGTCATTTGTC ATGGCTTCCATCTGTCCTGAGCAAGTCATTCCTTTGTTGCTAGGCATCTCCAGCATCCCTGCCATTAAAA GCCCTGCGTTCTCTGCACTGTTTGGCCAGCATAATCTCTAGCAACCTTTCCATGCTGAGTTTGAACCTGA CCATATGGACTGTATAAACAAGGGTTGCAGCCTCTGCATACACTCGAATCGCTACATTGCTTTTTCTATA AAAACTACCCATAAGCCTTTAACCTTTAATGCAAATTTTTAAAAGGTTAGTGTTTGGGGGGGGGGTGGGG ACTGACCTCGTCTTAAGCCAGTACATCTGAGCTGAGTGTTTTAATAAATCTGATATTTCTCAAGAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_022420
• Insert Size: 1233 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC020004, AAH20004
• RefSeq Size: 2899 bp
• RefSeq ORF: 1233 bp
• Locus ID: 64297
• UniProt ID: Q923Z0
• Cytogenetics: 7 F2
• Gene Summary: Unknown. This retinoic acid-inducible G-protein coupled receptor provide evidence for a possible interaction between retinoid and G-protein signaling pathways (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, which results in a frameshift, compared to variant 1. It encodes isoform 2, which has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.