Phf19 (NM_028716) Mouse Untagged Clone

CAT#: MC204253

Phf19 (untagged) - Mouse PHD finger protein 19 (Phf19), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Phf19
• Synonyms: 3110009G19Rik; 3321402G02Rik
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC027776
  CCACGCGTCCGGGCTGACGCGCTGCCTCGCGAAGCTAATGGAGACTCAAGCTCTGGAACCAGGGACTCTG GAAGCCTTTGGTGCCACCAGTCCTAACAAGGGGGGCCTGTCTAAGACCAAAAAGAACTTCAAAGACTTGA TGTCTAAGGTGACAGAGGGACAGTTCGTGCTATGCAGGTGGACAGACGGGCTATATTACCTTGGCAAGAT CAAGCGGGTCAGCAGTCCTAAGCAAAGCTGCCTTGTGACTTTTGAAGATAATTCCAAATACTGGGTCCTG TGGAAGGACATCCAGCATGCTGGTGTTCCGGGAGAGGAGCCCAAGTGTGACGTCTGCATGGGGAAGACTT CAGGGCCTATGAACGAGATCCTCATCTGTGGGAAGTGTGGCCTGGGTTACCACCAACAGTGCCACATCCC CATCGCAGTTGATGCCAACTGGCCCCTCCTCACTCATTGGTTCTGCCGACGCTGCATTTTCGCACTGGCT GTGAGGAAAGGTGGCGCTTTGAAGAAAGGCGCCATCGCCAAGACGCTGCAGGCAGTGAAAATGGTGCTGT CCTACCAGCCGGAGGAACTCGATTGGGACTCGCCCCATCGCACTAACCAGCAGCAATGCTACTGCTACTG CGGCGGGCCTGGAGAGTGGTACCTTCGGATGCTACAGTGCTACCGGTGTAGGCAGTGGTTCCATGAGGCT TGCACACAGTGCCTTAGTGAGCCTATGGTGTTTGGAGACCGCTTCTACCTGTTCTTCTGCTCCGTGTGTA ACCAAGGCCCAGAGTATATTGAGAGGCTGCCCTTGCGATGGGTGGATATAGTTCACCTGGCTCTCTATAA CTTGGGAGTACAGAGCAAGAAGCGGTACTTTGACTTTGAGGAGATCCTGGCCTTTGTCAACCATCACTGG GAGCTCCTGCAGCTTGGCAAGCTCACCAGCACCCCCATGACAGAACGAGGGCCACATCTCCTCAACGCTC TCAACAGTTACAAGAGCCGGTTCCTGTGTGGCAAGGAAATTAAGAAGAAGAAATGCATCTTCCGACTGCG CATCCGAGTCCCGCCTGCCCCTCCAGGAAAACTGCTTCCCGACAGGGCGTTGATGCCAAGTGACAAAGGG ACCTCCGAGCTGCTTCGTAAGAAAGGAAAGAGCAAGCCTGGTTTGTTGCCTCAGGAACCCCAGCAGCAGA AAAGGCGAGTTTATAGAAGAAAAAGATCAAAGTTTTTGCTGGAAGATGCTATTCCCAGTAGTGACTTCAC CTCAGCCTGGAGCACAGACCACCACCTAGCCAGTATATTCGACTTCACACTGGATGAAATTCAGAGTTTA AAAAGTGGCAGCTCAGGCCAGACCTTCTTCTCAGATGTGGATTCTACCGACGCAGCCAGCACCTCGGGGT CTGCCTCCACCAGCCTCTCCTACGACTCCAGATGGACGGTAGGCAGCCGCAAGAGGAAGCTGACAGCCAA AGTGCACAGGCCCCTGCGAGCAAAGCAAAGGGCGGCGGAGCTGGAGGGGCGCTGCGCCTCAGACAGCAAT GCAGAGGGAGCTGTGGGTCCTGAGCAGCCGGATGAAGGCATCGACAGCCACACACTTGAAAGCATCAGTG GAGACGACTCGTCCCTGTCCCACCTCAAGTCCTCTATCACCAACTACTTTGGTGCAGCTGGGCGGTTGGC CTGCGGGGAAAAATATCGGGTGTTGGCGCGGAGGGTCACTCCAGAAGGCAAGGTTCAGTACCTGTTGGAA TGGGAGGGGACCACCCCTTACTGATTACCCTCAGAGGCTGCCCAGATCCCTGCAAACCAAAGGAGGGGCA GAAGAGGCCACTGGGTCCCTGTTTTCGCCAGGCTTGGGGTGGTGGTGGTGAAACAGGACATCCTGCCTGT GGCCCCTGCCTGCTGCCTGCCAGGCCAAGGCCTGTGTGCTGCTGTACCTGAGTCTCTGAGACCCCATTGG TATTCTCTCGTTGTCAATACTACACTCCACACCCCTCAAACTTTTTATTGGTTCCTCTGACAGTATCCTG GCTCCAACCCCTGGATCTGTTTTTTGTTGTCCCCAATTCTCCATCTTCTGCCCTCTCACCCCTTTCAGGT GTTTTGTGTGGACACACATCTTCCACGGGCATACACCCATCTGTCCCTCACTGGGCATGCTTCACTGGGT CCTCACTAGGACCACAGTGAGGGAAGGGAACCTTGGGGGTCTTCTGCTTTCTCACTAGTACCCCTCACCC CAGGAAGGTAGGGGGTGCCTGAGAAAGTTTAGGATGCCTAGGACCCAGAGTCTGGGGAGTGGTGAGCTGG GTCTCTGCAGAAGGGACAAGAATCAGGGTCTTTTATTGAGGAGGGGAGACTAAGGAAGTCAGGCAGGGCC AAGGTTTCCAGAAGTTTGCCTGTCCCAGCAACAACCCTTTACTGTTGTCAAGGATTGGGACCTGGCCCTG CTGCATAGACGTGTCTTAAACACACAGATCTGGGAGGAAAGGATGGGGAAGAAGACTAAGCCAGATTAAA TCCCATCCCAAACACTGTGTGTACAGCCACCACCTACTCCTGCCAGGGCCCCCTGGCCCCCCGTCCTTGG CCTCTTCCAGGGATACATGCTGCAGTTAAATGTGAGAACCAAACCCCTGCCTCCTCTGGCCTGTGAAGCA GCCTTGGCCCCACAGAGAAGCACAAAATATACCCCTCTCCTGCATGGCTCCTGCCTTGCCCTGGAGCTGA CAATCACTTTGGGGACAGGTTTGGAGTTTACAAGGGCCTGAATCGTTGGTTCCCTTCAGAAAGGAAGGCT TTGTCATCAATGGTACAAATAGAATTCTTAGAATGGTCAAGCTTAGCCGTGTCTGGGGAGAAACCAGGAG GTGGGCAGGGAGCCCTTACTTTGCCTACAGAAACAGAAATTGACTCACTCAGTTGGAGAAATGGAAAGAA CTGCCATTGAAGTGGGAGGCAAGGGCACCAGACTGAGACTAGCCTCCCTGACTGCTGCTGTAGCTTCCAG CTGGGGGTGGGAAGATGGACCACATGGTGACATCAGACTATACCAAAGGTGCTGATATTCCCATGACTAG GTCTGACCCCTTCACACCTTCCCGTGAGCATTAAGGAGGGTCCCTGCAGTAGGTTAGATGTCCCCTGCCT TAAACCTCAGCCACCATGGTGCCTTGCAAAGAAACCTCGCCTCTCATAGACACAGCTACCTACTTCCCTC TGCCTTCCTGTCTGGGCTCAGGCAGGGATAAGCAGATGGACACTGGGCCCAGTTGTCAGTCCCACAGCTG CCGCCACTGAGGTCCCAAAGTGGGGCCTGGTGGAAGGAGAGTTGGGAAGCCAACTCCTAGGCTTGTGGGA ACTGGGGTCAAATTTGTGGCTTCCTGTTGGCTTCGGTGAACATGGCAACCTCCAACACGTGTTTTCCAGG CCACTGGGGAAGCCAGAGTTAGGATGGCTATCTGTACATCTGCCCCTCCTCTGCTGTGCCCCAGAAGCTC TGGGCCTGAGGAGCAGGAAGGCAGGAGGGGTGCCCGTCCCGTTGGGGATCCTAGCACAAGGCCTGACCAG GTCCCTGGACTTCCCATGGCAGCATTTCACTCCTACAAAATAATAGCTGCATTACTGCCAACTGATGGTA TAACCCTGTGCACCTTTGAAAAGATTTTGGTTTTAAATTGCTGCTTTTAATAATATTTGTTATATTAAAG TTATAATTGATGTGTCTGGTTTACAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_028716
• Insert Size: 1737 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC027776, AAH27776
• RefSeq Size: 3749 bp
• RefSeq ORF: 1737 bp
• Locus ID: 74016
• UniProt ID: Q9CXG9
• Cytogenetics: 2 B
• Gene Summary: Polycomb group (PcG) that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex. Probably involved in the transition from an active state to a repressed state in embryonic stem cells: acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting H3K36me3 histone demethylases RIOX1 or KDM2B, leading to demethylation of H3K36 and recruitment of the PRC2 complex that mediates H3K27me3 methylation, followed by de novo silencing. Recruits the PRC2 complex to CpG islands and contributes to embryonic stem cell self-renewal. Also binds dimethylated at 'Lys-36' (H3K36me2).[UniProtKB/Swiss-Prot Function]