Pola2 (NM_008893) Mouse Untagged Clone

CAT#: MC204033

Pola2 (untagged) - Mouse polymerase (DNA directed), alpha 2 (Pola2), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pola2
• Synonyms: AI573378
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC021424
  CCACGCGTCCGGTTTGGCGGGTTTTTCCTGTGCTGTGCTCCTCACGGAGAAGCTAAGGTGTAGTCCTCGG AAGCAAGTTTTCTCTCTGGATCGAGACCTCTCGAGGGCTTTTGAAGTATTTCTCTGTGACCTGGTGGCTG GGGTCGTTTGAGGGCTTAGGCCAGGTGGTCTCGTGACACGTCCCTGTCACCTTCCGGTAAAGCGAAAGGA TAAGCGTCTGCAGGGTAGAGCTGAGGTCGGGGCCCGGCGGCAGGCGCCCGGCCACTCCCGGGTCACCATG GCCGTGTCCACCCAGCAGCTGGCGGAGGAACTACAGATCTTCGGCCTGGACTACGAGGACTCCCTGTTGG AGAAACTGGCAGAGCTGTGTGTTCTATACCGACAGACTGAGGACGGGATGGTCAGTGAGCTCATCGCCTT CTGCACCAGTGCGGGGAAGACATGCCTTACTGTAGACATCCTGAACTCTTTTGAATACGAGGTTCTGAAC AAAAAACTCTCCAAAGCCTGGCACAGTGCCTCCAAAGACAGTGGGCATGCAGGAACCAGAGACATTGTTT CTATACAAGAGCTAATTGAAGCAGAGGAGGAAGAGGAGACCCTCCTAAGCTCTTATACCACACCCTCTAA GGGTCCTCTCAAGCGAGTTAGCAGCACCCCAGAAACCCCCCTAACCAAAAGGAGCGTCGCTGCTCGCAGC CCACGTCAGCTTCTCTCACCGTCAAGTTTCTCCCCGAGTGCTACCCCCTCCCAGAAATACACCTCCAGAA CCAACCGGGGAGAAGTGGTGACCACCTTTGGCTCCGCACAGGGCTTGTCTTGGTCCGGGAGAGGAGGCTC TGGAAGCGTCAGTCTAAAGGTCGTGGGGGACCCAGAGCCACTCACTGGCAGCTACAAAGCGATGTTTCAG CAGCTCATGGGTGTTCGAGAAGTTCTGACCAGTAAGATCGAAGAGCTCGGCAGCGAGCTGAAGGAGCATC ACAAGATCGAGGCCTTTACCCCTCTCCTGGTCCCAGCGCAAGAGCCTGTTATTCTCCTGGGTCAGATTGG CTGCGACAGCAACGGGAAGCTGAACAGCAAGTCCGTGATTCTCGAGGGGGACCAGGAGCACTCCTATGGC GCGCAGATCCCAGTGGACCTATCGGAACTCAAAGAGTATTCCCTCTTCCCTGGACAGGTTGTAATTATGG AAGGATTCAACACCACTGGTAGGAGACTTACTGCCACCAAACTCTATGAGGGCGTGCCACTTCCGTTCTA TCAGCCTACTGAAGAGGAGGGAGCTTCTGAGCAGACTATGGTGGTAGTTGCCTGCGGGCCTTATACCACC TCCGACAGCATCACCTATGACCCCCTGCTTGATCTGATCGCCATCATCAACCGTGACCAGCCAGATGTCT GCATCCTGTTCGGACCTTTCCTGGATGCTAAACATGAACAGGTGGAGAACTGTAAGTTGACAAGCCCATT CGAAGACGTTTTCAAGCAATGCCTGAGAACAGTTATCGAAGGAACGAGAAGCTCCGGCTCCCACCTTGTC TTTGTCCCGTCTCTGAGGGATGTCCATCATGAGCCAGTATACCCACAGCCGCCTTTCACCTTCTCCGAGC TGTCTCGAGAGGACAAAAAGCGAGTGCAGTTTGTGTCAGAGCCCTGCAGCCTCTCCATAAATGGAGTGAT GTTTGGTTTGACATCCACGGACCTGCTGTTCCACATCGGGGCTGAGGAGATCTTTAGCTCTTCCGGGACG TCAGACAGATTCAGCCGAGTCCTTAAGCATATCCTGACGCAGAGAAGTTACTACCCACTTTATCCACCCC ACGAGGACATGGCCATAGACTACGAGAACTTCTATACCTATGCCCAGCTGCCGGTCACCCCCGATGTCTT CATAGTGCCGTCCGAGCTGAGATACTTTGTGAAGGACATCTTTGGCTGTGTCTGTGTGAATCCTGGGCGG CTCACGAAAGGACAAGTGGGCGGCACCTTTGGCCGCCTCTACCTCAGGAGGCAGCCCAAGGCCATGGACG GTGGGGGCAGGCAGGGCCTGAGCGTGGCAGCGCAGGTGGTCAGAATCTGAGTCCTCAGCCTGGTTGCCTG GCCTCACAAACACCAAGAGCCCAGGTATGGCTCTCTGGTGGCCAAACTGTTGGAGTACCTGACAAGGATC TGGCCACAGAGACAGGACTGTAGGGCTGGGCCGTCCAGTCACGGCTTCCCACTGTCCCTCACCCCCATGC AGCGTGGCTCTGATCTGAATTACGCCCTGCTTCTCTCCTGCTTCATGAGGTAGATGTACAGAAGTGAGCC ATCTATGGACACAAAGGCAGGAAAACTGACTTTTTTTTGTATGAATCAAATACAGCGTGTGGAGAAATAA AAATTTTTTTAGTATAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_008893
• Insert Size: 1803 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC021424, AAH21424
• RefSeq Size: 2410 bp
• RefSeq ORF: 1803 bp
• Locus ID: 18969
• UniProt ID: P33611
• Cytogenetics: 19 A
• Gene Summary: May play an essential role at the early stage of chromosomal DNA replication by coupling the polymerase alpha/primase complex to the cellular replication machinery.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).