Smyd2 (NM_026796) Mouse Untagged Clone

CAT#: MC204022

Smyd2 (untagged) - Mouse SET and MYND domain containing 2 (Smyd2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Smyd2
• Synonyms: 1110020E07Rik; 4930402C15; KMT3C; Zmynd14
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC023119
  GCCGCCAGCATGCGCGCCGAGGCCCGCGGCGGCCTGGAGCGCTTCTGCAGTGCGGGCAAGGGCCGGGGGC TCCGCGCGCTGCGGCCCTTCCACGTGGGCGACCTCCTCTTCTCCTGCCCGGCCTACGCCTGCGTGCTCAC CGTGGGCGAGCGGGGCCACCACTGCGAGTGCTGCTTCGCCAGGAAAGAAGGATTGTCAAAATGTGGACGG TGCAAGCAGGCGTTCTACTGCGACGTGGAATGTCAGAAGGAAGACTGGCCCCTGCACAAGCTGGAATGCT CCTCCATGGTTGTTTTGGGGGAGAACTGGAATCCTTCGGAGACTGTGCGGCTCACAGCAAGAATTCTGGC CAAACAGAAAATCCACCCAGAGAGGACACCTTCAGAGAAATTGTTGGCTGTGAGGGAGTTTGAGTCACAT CTGGACAAGCTAGACAACGAGAAGAAAGATCTCATCCAGAGTGACATCGCCGCTCTCCACCAGTTCTACT CCAAGTACCTGGAATTCCCTGACCACAGCAGTCTCGTGGTGCTCTTCGCCCAGGTGAACTGTAATGGCTT CACTATTGAAGATGAAGAGCTCTCCCATTTGGGATCGGCGATATTCCCTGATGTTGCATTGATGAATCAC AGCTGCTGCCCGAATGTCATTGTGACCTACAAAGGGACCCTGGCAGAAGTCAGAGCTGTACAGGAGATCC ACCCAGGAGACGAGGTGTTCACCAGCTACATCGACCTGCTATATCCAACAGAAGACAGGAACGACCGGCT AAGAGACTCCTATTTCTTTACCTGTGAGTGCCGGGAGTGTACAACCAAGGATAAGGACAAAGCCAAGGTG GAAGTCCGAAAGCTCAGCAGCCCGCCACAGGCAGAAGCCATCCGAGACATGGTCAGATACGCACGCAATG TCATCGAGGAGTTCCGGAGGGCCAAACACTACAAATCCCCTAGTGAGCTGCTGGAGATCTGCGAGCTTAG CCAGGAGAAGATGAGCTCCGTGTTTGAGGACAGCAATGTGTACATGCTGCACATGATGTACCAGGCCATG GGCGTCTGCCTGTACATGCAGGACTGGGAGGGAGCCCTGAAATACGGGCAGAAGATCATTAAGCCGTACA GTAAGCACTACCCCGTGTACTCCCTCAACGTGGCTTCCATGTGGCTGAAGCTGGGAAGACTGTACATGGG TCTGGAAAACAAAGCGGCCGGCGAGAAGGCCCTGAAGAAGGCCATTGCCATCATGGAGGTAGCTCACGGC AAGGACCATCCGTACATCTCTGAAATCAAGCAGGAGATTGAGAGCCACTGACTGCACCCCAGCTTTCCGT CTGCGACCTCACTCACAGGACTTGAGTATTTCCGACCTCACGGGTCACCCCAGCCCTCTGCAAAGCCTTG CCGTGCTATGTTTCAGGTTCACGTTCAGCTCTGACCTTGAGTTCATTTCCAGTGCTTGCCTTCTCTCCAA GGCCATGGCATGAGTTCACAGAATATATATTTTCGGCAGATTGAGCTTTAGAGATGCAACGACTTTTCCC TCTGTGCCTGTAGGAATGCTCAGTGGAGCTGACCGAGGAAGAAAATAAAAGAGCCAGTCATTGAAAAAAA AAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_026796
• Insert Size: 1302 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC023119, AAH23119
• RefSeq Size: 1619 bp
• RefSeq ORF: 1302 bp
• Locus ID: 226830
• UniProt ID: Q8R5A0
• Cytogenetics: 1 H6
• Gene Summary: Protein-lysine N-methyltransferase that methylates both histones and non-histone proteins, including p53/TP53 and RB1. Specifically methylates histone H3 'Lys-4' (H3K4me) and dimethylates histone H3 'Lys-36' (H3K36me2). Shows even higher methyltransferase activity on p53/TP53. Monomethylates 'Lys-370' of p53/TP53, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity of p53/TP53. Monomethylates RB1 at 'Lys-860'.[UniProtKB/Swiss-Prot Function]