Pomk (NM_029037) Mouse Untagged Clone

CAT#: MC204007

4930444A02Rik (untagged) - Mouse RIKEN cDNA 4930444A02 gene (4930444A02Rik), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pomk
• Synonyms: 4930444A02Rik; Sgk196
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC027296
  ATTTGGTGGTCGCCAGCATTTTGTGAAAGAAGGAAGATCCTGTCTGCTGCATGGAGAATGGACTGGAACA CAGAAAGACCATGCAGGGTTCGGCTGTAGAAGGCCGGTATCTCCAGAGGCCAGAAGACACCATCAGATCT CCTGGGACTGGAGTTATAGAGGTTGTGAGCTGCCATGTTGAACCAAGCAGGTCACTGAGGGACACAGGCA TGTGGATGGAAACCCTGCTGGGAGAAAAAAGAAACTGCTGAAGGGACTGACATGGGACAGCAACATGGAA CCAGGAATGGTCTCACGCATAGAGAGCTCCCCCGGGGCGTGGGGCTGCTGCTCGCCATGGCCCTTATGAA CGTGGCGCTCTACCTCTGCCTCGATCAGCTTTTCATCTCCCCTGGACGATCCACCGCGGACTCTAGGCGC TGTCCTCCAGGCTACTTCAGAATGGGGCGGATGAGAAACTGCTCACGCTGGTTGTCCTGTGAAGAGCTGA GGACAGAAGTCAGGCAGCTGAAGCGCGTTGGGGAGGGAGCCGTGAAGAGAGTCTTTCTGTCTGAATGGAA GGAACACAAAGTCGCTCTCTCCCGGCTCACCAGGCTGGAGATGAAGGAGGACTTCCTGCATGGGCTGCAG ATGCTGAAGTCTCTACAGAGTGAGCACGTGGTCACGCTGGTGGGCTACTGTGAGGAAGATGGCACTATTC TCACCGAATATCACCCCTTAGGTTCCTTGAGCAACCTGGAAGAAACACTAAACCTTTCAAAGTACCAAGA CGTGAACACTTGGCAGCACAGGCTGCAGCTGGCCATGGAGTACGTCAGCATCATTAACTATCTGCATCAC AGCCCCCTGGGCACGAGGGTCATGTGTGACTCTAACGACCTGCCCAAAACATTGTCCCAGTACCTGCTAA CAAGTAACTTCAGCATTGTGGCAAACGACCTGGACGCGCTGCCCCTGGTAGACCATGACTCTGGGATACT TATAAAGTGTGGCCACAGAGAGCTCCATGGGGATTTCGTGGCTCCGGAGCAGCTGTGGCCCTACGGAGAA GACACGCCCTTCCAAGACGATCTCATGCCTTCCTACAATGAGAAGGTTGACATCTGGAAGATTCCAGATG TCTCCAGTTTCCTCTTGGGGCACGTGGAAGGGAGTGATATGGTTAGATTCCATTTGTTTGATATCCATAA GGCGTGCAAGAGCCAGATCCCAGCAAAAAGACCCACTGCTCAGAACGTGCTAGACGCTTACCAGAGGGTT TTCCATTCACTCCGAGACACTGTGATGTCGCAGACGAAAGAAATGCTGTAAGAATGAGCCATCGAGTGAC GTGCTTGACGGCTGAATGGCATCCCAGCTGTTCCGCTCTTGATGATGGAAGAGCTTTGCATGGATGGATG TTGACCCTGGCTGTTCAGCCACGTAGGCCTCCTCTACGTCTGCCTGCATGTTTGAGTGTTCTGCTCTCCT GGCAGCCCGGACGGAAGCTGCCAAGCGAGAAAGCCTGGCTTCAGGATGCTCCCTGGTGAAGATGCAGAGG ATTCTGGATCTGCATAGTTTCAAGGGAGTGATCAAACGGTGACCTTGAAGACATGCTGCCTGCCCTGGTA ACTTTTTATAGACTAGTAGGAAACAAATCTTTCGGGGGGGGGCAACCCACTAGTTCCTCAGAGACAATTT CTTCTCATTCAGAAAGCCCTGTTGGAAGCTGGGGATGTTTTAACTCCGTGGCAGGGCACTTGCCTAGTTG TGTGCAAAGCCTTGGATCTGACCCATGGCATGTGCACACACACAAATGCTCAAAGAAAATCCCAGACGCC AGAAGTGTGCCCCTTTCTTGTCAATAAGGTCATTGTCAGTACCGGAGATGATTTTTTTTATGAAGCGTTT ATGCTGACTCCTGTCACTGAGCCAAGTGTGCATGGTCGTTAGCTACTTTGTGGGTTCTTCTTTCTTCTAC CCTACTTCTTCCCTTTCCACCCCCTAACACTAGATAGAAGAGAGGAGAGAGAAAGGAAAGTGGGCACTGT TATATTGTTGGACGACTTCTTGCTGATTAAGGGGTGTTAAGTTCCTTGGAGCAATGATCTTTGCTGCCAA GATATCTCATTTCTTCTTATTTCTTCTTCGCCCACGACCACTTCACAAACACTGACCAACAGCAAACAAC AAACAACCCAACAGCAAACAACAAACAACCCAACAGCAAACAACAAACAACCCACCCCGCCTCTCGGGGG CCCTAGCACTTATGTACTTCTGAAAAGTCCCCAGAAATTCCAATCATCACACACTCACAGAAACTGTCTG CTGCTGGCAAAACTACACCCCTGCTAGAGCATGAGGCAAATCATAGTCAGCTGCTGTGGACAGTCTGAAG CATCCTGGCATCCCACACCTGAGATTAAAACAAAAACATTCTTACCTGTGTTTTGTTTTTGTTTTAAGAA ACCAAAGTGCACCAAGATAGCATGCTCTTGAGATTGTGACTGTCTAGAGATTTTTGGAACAGCAAGTTGA AAGAACTTTCTTACCTGCCTTGAATGGTGCTTTGAACTTCCTGCTGACCTGGAGTTTCTGTGTGAATATT TCTATCCAGTGTCCCCCTGTACCGGAAAGTACAAAGTCTGCTCTGGGCTTGCATGCCTGAACACTTTAAA ACACTGTGGAGCCAGGAATAATGGTACTCACCTGTAATCCCAGCACCTGGGAGACAGGAGGAACCAGGAG TTCAGGGTTATCCTGGGCTATATACCGTGACCCTGTCTACCCCCACACCCCAATAAAAAAACAAAAAGGT CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_029037
• Insert Size: 1050 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC027296, AAH27296
• RefSeq Size: 2885 bp
• RefSeq ORF: 1050 bp
• Locus ID: 74653
• UniProt ID: Q3TUA9
• Cytogenetics: 8 A2
• Gene Summary: Protein O-mannose kinase that specifically mediates phosphorylation at the 6-position of an O-mannose of the trisaccharide (N-acetylgalactosamine (GalNAc)-beta-1,3-N-acetylglucosamine (GlcNAc)-beta-1,4-mannose) to generate phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-1,3-N-acetylglucosamine-beta-1,4-(phosphate-6-)mannose). Phosphorylated O-mannosyl trisaccharide is a carbohydrate structure present in alpha-dystroglycan (DAG1), which is required for binding laminin G-like domain-containing extracellular proteins with high affinity. Only shows kinase activity when the GalNAc-beta-3-GlcNAc-beta-terminus is linked to the 4-position of O-mannose, suggesting that this disaccharide serves as the substrate recognition motif (By similarity).[UniProtKB/Swiss-Prot Function]