Atp5a1 (NM_007505) Mouse Untagged Clone

CAT#: MC203883

Atp5a1 (untagged) - Mouse ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1 (Atp5a1), nuclear gene encoding mitochondrial protein, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Atp5a1
• Synonyms: AI035633; AL022851; AL023067; Atpm; D18Ertd206e; Mom2
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC014854
  GCTGCGGCTGCAGACGGTCTCTCCGAGAAGCTGCAAGGATGCTGTCTGTGCGCGTCGCCGCGGCCGTGGC CCGTGCCCTCCCTCGACGGGCGGGACTGGTCTCCAAAAATGCTTTGGGGTCATCCTTTGTTGGTGCAAGA AATCTCCATGCCTCTAACACTCGACTTCAGAAGACTGGCACAGCTGAGATGTCCTCCATTCTCGAGGAGA GGATTCTGGGAGCTGACACGTCTGTTGACCTGGAGGAGACTGGGCGTGTGTTAAGCATTGGTGATGGTAT TGCGCGAGTGCACGGGCTGAGGAATGTTCAAGCAGAGGAGATGGTGGAATTCTCTTCAGGCTTAAAGGGT ATGTCCCTGAACTTGGAACCCGACAATGTTGGAGTTGTCGTGTTTGGGAATGACAAGCTAATTAAAGAAG GAGACGTTGTGAAGAGGACAGGCGCCATCGTGGATGTCCCCGTTGGCGAGGAGCTGTTGGGCCGTGTGGT TGACGCCCTCGGTAATGCTATTGATGGAAAGGGTCCAATTGGTTCCAAGACCCGCAGACGAGTGGGCCTG AAAGCCCCTGGAATTATCCCCCGAATCTCTGTGCGGGAGCCGATGCAGACCGGCATCAAGGCTGTGGATA GCCTGGTGCCCATTGGCCGGGGTCAGCGTGAGCTGATTATTGGTGACAGACAGACTGGGAAAACATCGAT TGCCATTGACACAATCATCAACCAGAAACGTTTCAACGATGGGACCGACGAGAAGAAGAAGCTGTACTGC ATCTACGTCGCGATTGGTCAGAAGCGGTCCACTGTGGCTCAGCTGGTGAAGAGACTGACGGATGCGGATG CCATGAAGTACACCATCGTGGTGTCAGCCACTGCCTCTGACGCTGCCCCGCTTCAGTACTTGGCTCCCTA CTCCGGCTGCTCCATGGGAGAGTACTTTAGAGACAACGGCAAGCACGCTTTGATCATCTATGACGATCTA TCCAAGCAGGCTGTCGCTTACCGCCAGATGTCTCTGTTGCTCCGCCGACCCCCTGGTCGTGAGGCCTATC CTGGTGATGTGTTCTACCTACACTCCCGCCTGCTGGAGAGAGCAGCCAAGATGAACGATTCCTTTGGTGG TGGCTCTTTGACTGCCTTACCAGTCATTGAAACACAGGCTGGTGATGTGTCCGCTTACATTCCAACAAAT GTTATTTCCATCACTGACGGACAGATCTTCTTGGAAACAGAATTGTTCTATAAAGGCATCCGCCCTGCCA TTAATGTGGGTTTGTCTGTGTCCCGTGTCGGATCTGCTGCCCAAACCAGAGCCATGAAGCAGGTGGCAGG TACCATGAAGCTGGAGTTGGCTCAGTACCGGGAGGTGGCTGCGTTTGCCCAGTTTGGTTCTGATTTGGAC GCTGCCACTCAACAGCTCTTGAGTCGTGGTGTGCGTCTGACCGAGTTGCTAAAGCAAGGACAGTACTCTC CCATGGCTATTGAAGAACAGGTGGCTGTTATCTATGCGGGTGTACGGGGTTATCTTGACAAACTGGAGCC CAGCAAGATCACAAAGTTTGAGAATGCTTTCTTGTCTCACGTTATCAGCCAGCACCAGTCCCTCTTGGGC AATATCAGGTCTGATGGGAAAATCTCAGAACAGTCAGACGCAAAGCTCAAAGAGATTGTAACAAACTTCT TGGCTGGGTTTGAACCATAAAGCCCTGTCACTGTCACCAGATACTGCTTTGGTTTTGTCATTTATTCTGG TAAAATCAGCACCATTTGTAAAGGGTTACTCTTGTATTCCTGATGTACAGAAATCACATGAATAAAAGTT CCATACTGTGTGCTAGTTTGTGTGGGGGTGGGCTCAGGTGTGTACCAGGATTTGTGTGGTGGTCAGAGGA CAGCCAGCCTGTATGACAGTACCCTCCTTCCACCGGGAATTTAATGCAGGTTTTTATTTAAAGCACGACT TCATGTTGAGTTCCATAAGTTAGTTTTGTAAGACAGAGCTTACTGTGTGACCTCAGCAATGGCTAGCCTA GGCTATTCTTGTAGCAGTCTGTCTGCCATAGCTGGCAAATTGCTCTCTTACCCTGTTCTTGCTGGTGCAG TCAGCATGGATAGATAATTCAAAGCTGTCCACTATGAAATTCGGAAGTACTTTGTGATTGATTAAAAAGA GAACTCTGCTCCGAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_007505
• Insert Size: 1662 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC014854, AAH14854
• RefSeq Size: 2202 bp
• RefSeq ORF: 1662 bp
• Locus ID: 11946
• UniProt ID: Q03265
• Cytogenetics: 18 52.38 cM
• Gene Summary: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites (By similarity). Binds the bacterial siderophore enterobactin and can promote mitochondrial accumulation of enterobactin-derived iron ions (By similarity).[UniProtKB/Swiss-Prot Function]