Grem1 (NM_011824) Mouse Untagged Clone

CAT#: MC203822

Grem1 (untagged) - Mouse gremlin 1 (Grem1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Grem1
• Synonyms: Cktsf1b1; Drm; Grem; ld
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC015293
  CCAGAGTACCGTGGTAGCGGCGCTCTCCTTCGTCTTCCTCCCGCGTCGAGCCCAAGTGGCTCGAGCCGCG GTCTCACGCAGCGCCACTCGTCCACAGCGAAGAACCTGAGGACCCACGGAAGTGACAGAATGAATCGCAC CGCATACACTGTGGGAGCGTTGCTTCTCCTCCTGGGGACCCTACTGCCAACAGCTGAGGGGAAAAAGAAA GGTTCCCAAGGAGCCATTCCGCCTCCTGACAAGGCTCAGCACAATGACTCTGAGCAGACCCAGTCCCCAC CACAACCTGGCTCCAGGACCCGGGGGCGGGGCCAGGGGCGGGGCACCGCCATGCCTGGAGAGGAGGTGCT TGAGTCCAGCCAAGAGGCCCTGCACGTGACAGAGCGCAAGTATCTGAAGCGAGATTGGTGCAAAACTCAG CCCCTGAAGCAGACCATCCACGAGGAGGGCTGCAACAGCCGCACTATCATCAACCGCTTCTGTTATGGCC AGTGCAACTCCTTCTACATCCCCAGGCACATCCGAAAGGAGGAAGGGTCCTTTCAGTCTTGCTCCTTCTG CAAGCCCAAGAAGTTCACCACCATGATGGTCACACTCAACTGTCCTGAGCTACAGCCACCCACCAAGAAG AAAAGGGTCACACGCGTGAAGCAGTGCCGTTGCATATCCATCGACTTGGATTAAGTCAAAGCGGGCACAT TCAGCCTGTCATAGCCATGCTGAGAGAGCCACACCCAAACCACCCGATTCCTACTTGGCTTAAACCTAGA GGCCAGAAGAACCAGCAGTTGCTTCCTGGCTGGAGGCTGCTTATGCATAGTGTATGCGCATGAGTGTGCA TGGGTGCCTGTGGGTGTTTCCAAACACCAGCCGGAAACAGCCTTTGCTAGAAGGCACTTCCTGTTACTCT GCTTCAGATGGTCGGAAATGCCCACACCACTGGACCCAAACATCCACAGGGGCAGGGCTGTAGTTGGCTT TGTCATTGTGTTCCATGTGCCTCCTGGGCACCAGGATTTCACTTGAGAATGAATACTAATGGGGGAGGTA ACTCTGAGGGCTGCATTAGACTCGGAACTGTTCAGTGCTCGCCCTATGCTCCCATAGCCCATCCCTTTCT TTGCTCTCCCTGACATCTCAGTCGTAGCCCATGTTCCTAAATTAATTCACTTGACCGCGGGTGTAAGTCT TTTGTCTTGTGAAGAACCTTCAGAATGTGGGGAGACACGTGGTGATGGCAAACGGGACAGAGGACTGACG CAGGAACGGTCAGGCTGAGGACCAGTCTGGGCCAGTGAAATTCAGTAGTGAGATGTCTAGAGTTTAAAAG TTGTTTCCCAAAACAATATTAGTCTTGTTTTTAGCAAAAGGGTTTTCCTGATATTTAAAAGAACCCAGAC ACACAGAGGAAAAATATAATCAGCAAAAAAACAAAACAAAACAAAATAACACAACAATAACAACAACAAC AAACAAAAACCCAATTCTCTGTGCCAGCTTCTGTGACCTACTGATACTAGCTGTAACTGATACTAGCTGT TAAGGGTGAAATGCTGACCACTCCTGTTTTAAGAACCAAGTGAAATTAAAAAAGAAAATGTGGCCTCCTA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_011824
• Insert Size: 555 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC015293, AAH15293
• RefSeq Size: 1674 bp
• RefSeq ORF: 555 bp
• Locus ID: 23892
• UniProt ID: O70326
• Cytogenetics: 2 57.43 cM
• Gene Summary: Cytokine that may play an important role during carcinogenesis and metanephric kidney organogenesis, as BMP a antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop (PubMed:12808456, PubMed:15201225). Down-regulates the BMP4 signaling in a dose-dependent manner (PubMed:15133038). Antagonist of BMP2; inhibits BMP2-mediated differentiation of osteoblasts (in vitro) (By similarity). Acts as inhibitor of monocyte chemotaxis (By similarity).[UniProtKB/Swiss-Prot Function]