Mymk (NM_025376) Mouse Untagged Clone

CAT#: MC203616

Tmem8c (untagged) - Mouse transmembrane protein 8C (Tmem8c), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mymk
• Synonyms: 1110002H13Rik; AI131587; myomaker; Tmem8c
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC062145
  TTTTTCTTCCTGCCCTGGAGACCAGGGGGGCCTTTACCACCTTCTCCCCAGTCAGTGCCTTCCTCCTGGT CCATGGGGACAGTTGTAGCCAAACTGCTCCTGCCTACCCTCAGCAGCCTGGCCTTCCTCCCGACAGTGAG CATCGCTACCAAGAGGCGTTTCTACATGGAGGCCATGGTCTACCTCTTCACCATGTTCTTTGTGGCGTTC TCCCATGCCTGTGATGGGCCTGGTTTGTCTGTGCTGTGCTTCATGCGCCGTGACATTCTGGAGTACTTCA GCATCTATGGAACAGCCCTGAGCATGTGGGTCTCCCTGATGGCACTGGCCGACTTTGATGAACCCCAGAG ATCGACCTTCACAATGCTTGGCGTCCTTACCATCGCTGTGCGGACTTTTCATGACCGCTGGGGTTACGGG GTATACTCCGGTCCCATAGGCACGGCCACCCTCATCATTGCTGTAAAGTGGCTGAAGAAGATGAAAGAGA AGAAGGGCCTGTACCCCGACAAGAGCATCTACACCCAGCAGATAGGCCCCGGCCTGTGCTTTGGGGCCCT GGCCCTGATGCTTCGATTCTTCTTTGAGGAATGGGATTACACCTACGTCCACAGCTTCTACCACTGTGCC CTGGCCATGTCCTTTGTCCTGCTGCTGCCCAAGGTCAACAAGAAGGCTGGGAACGCAGGGGCCCCCGCCA AGCTGACCTTCTCCACCCTCTGCTGCACTTGTGTCTGACTATACCCCCCCCCACACACACACACACCAGG CCCCTGCCTTCCTGCCTGGCAGTCCTGCTGTCTCTCCCAAGGTACTTCCTATACTTTGTTATGCGGCCTG TACATGAGAAATGGTCTTCTCTACACCCCAAGAGACCAGCAGGCCTGCTGCATTCTGCTGAGTGCTGCTT AGGGACCCACTGGTTCTGTGTTCACCAGTTGCTTCACTCTGTTCAGGAAAAAAAAGAACTTTATCCCCCA AGGCCTCACAACCATAGGTGTGCCTGGCAGAGAACCCTAGACCAGTAAATACCCAGCAGCATGCAGGGTT ATCTATTTCCCAGGTCCTGCCTGTCAGAATCGTCTGCTTTCCCTAGGAAACTGGGATTGCCCTTTTAACC CCTGCCCAGGCTCTGAAAGCTCTCCACTTAGGAAGCTGGAGCCAGCGAACCTTGCATACCCCTGCCTGAG TCCCATCCCTTCTGCAGGTTTTCGATCAAGCCATTCACAGTAAACTCTTGATCAGCCACTAGTCATTAGC TTCGCCTAACATACATTCTAGTTCCTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_025376
• Insert Size: 666 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC062145, AAH62145
• RefSeq Size: 1327 bp
• RefSeq ORF: 666 bp
• Locus ID: 66139
• UniProt ID: Q9D1N4
• Cytogenetics: 2 A3
• Gene Summary: Myoblast-specific protein that mediates myoblast fusion, an essential step for the formation of multi-nucleated muscle fibers (PubMed:23868259, PubMed:28386024, PubMed:28681861, PubMed:30197239). Actively participates in the membrane fusion reaction by mediating the mixing of cell membrane lipids (hemifusion) upstream of MYMX (PubMed:30197239). Acts independently of MYMX (PubMed:30197239). Involved in skeletal muscle regeneration in response to injury by mediating the fusion of satellite cells, a population of muscle stem cells, with injured myofibers (PubMed:25085416). Also involved in skeletal muscle hypertrophy, probably by mediating the fusion of satellite cells with myofibers (PubMed:28186492).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).