Pan2 (NM_133992) Mouse Untagged Clone

CAT#: MC203510

Pan2 (untagged) - Mouse PAN2 polyA specific ribonuclease subunit homolog (S. cerevisiae) (Pan2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pan2
• Synonyms: 1200014O24Rik; AI047843; AW742773; mKIAA0710; Usp52
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC075686
  AGGCGGGGGAGTAGTAGGGAAACTGGAATTTCCCGCGGAGCTGACGGCGCTTTCTCTACCCCCTACTCGT TTGAATTCCACGCGCTCCAAAATATCCGCCATGGAGAAATCTTGGCCAGAATGTCCATTCTAGGTTCACT GGTGCTGTCCTCCTGAAGGCTGGGTCACTTGTCTGTAGGAAGCGTGGCAAGGGAGGGTGTGACTCCGGTC TGACCAGCGGCCACTATCGTCATGAACTTTGAGGGTCTGGATCCTGGACTGGCCGAATTTTCCCCAGCTA TGCATTCTACCCTGGACCCAGTCCTGGATGCCCACCTGAATCCAAGTTTGCTACAGAATGTGGAGCTAGA CCCAGAGGGAGTGGCCTTGGAGGCTCTTCCTGTCCAGGAGTCAGTGCACATAATGGAAGGTGTCTACTCT GAGTTGCACAGCGTGGTGGCTGAAGTGGGAGTGCCTGTGTCTGTCTCCCACTTTGATTTACATGAGGAGA TGCTGTGGGTGGGGAGCCATGGGGGCCATGCCACCTCCTTCTTCGGCCCAGCTCTGGAGCGCTACTCATC CTTTCAGGTCAATGGTGGTGACGACATTCGGCAAATCCAGAGCCTGGAGAACGGTATCCTCTTCCTCACC AAGAACAACCTCAAGTACATGGCCCGTGGAGGGCTCATTATATTTGACTATTTGCTGGATGAAAACGAGG ATATGCACAGCGTCCTCCTGACAGACAACAGCACTCTGCTCGTTGGGGGGCTGCAGAACCACGTACTGGA GATTGACCTGAACACTGTCCAGGAGACTCAGAAGTATGCAGTCGAGACACCCGGAGTCACCATCATGAGA CAGACAAATCGTTTCTTCTTCTGTGGCCACACATCTGGCAAGGTTTCCCTGCGAGACCTCCGTAGTTTTA AAGTGGAGCATGAATTTGATGCCTTCTCAGGGAGTCTGTCAGATTTTGATGTTCATGGCAACCTGCTGGC TGCCTGCGGCTTCTCCAGTCGCCTTACCGGCCTGGCCTGTGACCGTTTCCTCAAAGTGTACGATCTGCGC ATGATGCGTGCCATCACACCTCTTCAAGTGCACGTGGATCCGGCCTTCTTACGATTCATCCCCACATACA CTTCCCGCCTTGCTATCATCTCCCAGTCAGGTCAATGCCAGTTTTGTGAACCCACAGGCCTGGCCAACCC AGCCGACATTTTCCATGTGAATCCCGTGGGACCTTTGCTAATGACGTTTGATGTGTCAGCCAGCAAGCAG GCCCTGGCCTTTGGGGATTCTGAGGGCTGCGTGCATCTCTGGACTGATTCCCCTGAGCCGTCCTTCAACC CCTACTCCCGAGAGACTGAGTTTGCCCTGCCCTGTCTTGTGGACTCCTTGCCGCCTCTGGACTGGAGCCA GGATCTGCTGCCGCTTTCCCTCATTCCCGTCCCACTTACCACTGATGCACTGCTCTCTGACTGGCCTGCT GCCAACTCTGCTCCCGCTCCCAGGCGAGCACCACCTGTGGATGCAGAAATTCTTCGAACCATGAAGAAAG TGGGCTTCATTGGCTATGCTCCCAACCCTCGCACCAGGCTGCGCAACCAGATTCCCTATCGACTAAAGGA GTCAGACCATGAATTTGACAACTTCAGCCAAGTCACGGAGTCACCGACAGGGCGAGAAGAGGAGCCTCTC CACACAGTTTCTAAGAAATACCGGAAGGTAACCATCAAATATTCCAAGCTAGGCCTGGAAGACTTTGACT TCAAACACTACAATAAGACTCTGTTTGCTGGGTTAGAGCCTCACATCCCCAATGCCTACTGTAACTGCAT GATCCAGGTGCTCTATTTCTTGGAGCCTGTTCGCTGTCTGATCCAGAACCACCTTTGCCAAAAGGAGTTC TGCCTAGCGTGCGAGCTCGGCTTCCTCTTCCACATGCTAGACCTCTCTCGTGGCGATCCTTGTCAGGGCA GTAATTTCCTCCGAGCTTTCCGGACCATCCCTGAGGCCTCAGCTCTGGGTCTCATCCTTGCTGACTCAGA TGAGGCTTCAGGCAAGGGCAGTCTGGCCCGGCTCATCCAGAGGTGGAACCGCTTCATCCTCACTCAGCTA CATCAGGATATGCAGGAGCTGGAAGTGCCCCAGGCTTATCGAGGTGCTGGAGGCAGTTTCTGCTCATCAG GAGACTCCATCATTGGGCAGCTGTTCAGTTGTGAGATGGAGAACTGCAGTCTTTGCCGCTGCGGCAGCGA GACTGTGCGGGCCTCGTCCACCCTACTCTTCACACTCTCCTACCCTGAGGATAAAACCGGGAAGAACTAT GACTTTGCTCAGGTGCTGAAGCGAAGTATCTGCCTGGAGCAGAACACACAGGCCTGGTGTGACAACTGTG AGAAGTACCAGCCCACAATTCAGACCAGAAACATCCGACATCTGCCAGATATTCTTGTCATTAACTGTGA AGTGAATAGCTCAAAAGAGGCTGATTTCTGGAGACTGCAAGCTGAGGTTGCCTTCAAAATAGCGGTGAAA AAGTACGGCGGGGAAATGAAGAGCAAAGAGTTTGCTTTAGCTGATCGGAAGGAACTGAGGAGTCCAGAGG GCTTTCTGTGTTCCTCCATCGAGGAGCTGAAGAATGTCTGGCTTCCATTTTCCATCCGCATGAAGATGAC CAAGAACAAAGGACTGGATGTTTGCAACTGGGCTGACGAGCATGAGCTTAGCAGCCTGGGTGCCCCCTCA CAGTGGGGTCCGGCCAGGGCAGAGGAGGAGCTTGGTGTCTATGTATATGACCTGATGGCTACTGTGGTAC ACATCCTGGACTCACGAACAGGCGGCAGCTTGGTGGCTCACATCAAAGTCGGTGAGACCTACCACCAGCG CAAGGAGGGCGTGACTCATCAGCAGTGGTATCTCTTCAATGACTTCCTTATTGAACCTATTGATAAGTAT GAAGCCGTGCAGTTTGACATGAATTGGAAAGTACCTGCTATCCTGTATTATGTCAAAAGGAATCTCAATT CCAGATACAATCTGAATATCAAGAACCCCATTGAGGCCAGTGTGCTGCTGGCAGAAGCCTCGCTGGCACG GAAGCAACGGAAAACACACACGACCTTTATTCCATTGATGCTGAATGAGATGCCACAGGTCGGGGACCTG GTGGGCCTGGATGCCGAGTTTGTCACTCTTAATGAGGAAGAAGCAGAGTTACGCAGTGATGGTACCAAGT CCACCATTAAGCCAAGCCAGATGTCAGTAGCAAGGATCACCTGTGTTCGGGGCCAAGGGCCTAACGAGGG CATCCCCTTCATTGATGACTACATCTCCACGCAGGAGCAGGTAGTAGATTACTTGACTCAGTACTCGGGG ATAAAGCCAGGAGACCTTGATGCCAAAATCTCCTCAAAGCACCTCACAACCCTCAAGTCTACCTACTTAA AGCTTCGCTTTCTCATTGATATTGGAGTCAAGTTTGTGGGTCATGGTCTGCAGAAGGACTTCCGGGTCAT CAACCTCATGGTGCCCAAGGACCAAGTTCTTGACACAGTCTACCTGTTCCACATGCCCCGAAAACGAATG ATTTCCCTACGATTCCTGGCCTGGTATTTTCTAGACCTGAAGATTCAAGGTGAGACCCATGACAGCATTG AGGATGCCCGCACAGCCCTTCAACTCTACCGGAAGTATCTGGAGCTGAGCAAGAACGGCACCGAGCCCGA GTCCTTCCACAAGGTGCTCAAGGGTCTCTATGAGAAGGGCCGAAAGATGGACTGGAAGGTGCCTGAGCCT GAAAGCCAGACAAGTCCCAAGAATGCAGCTGTCTTCTCAGTGCTGGCACTGTGAGCTGCCTTCCCTTTCA GTGCTGTCCCCAGAACTGAGAGGTGGCTTCTCAAGTCGGCTTTACCCTGTCCACTTCCAGAACTGGATCT GCTCAGGGTCTACAGATGGTGCTATTAATAGAACTGGAAAGCAGCAAAATTGTTGCAAAGGTTCTAGCTG CCAGATTCCCCCTTTATCCTCTGCAAAACAGTGGGCCAGAAACAAAGCCTAAAGTGACCCAGGTGGAGTG ATTGGTGTTCGTAATAAATATCCTGGGCAACTGATATCTGTACAAAAGAGCTCTAGAACCAGAACTCTCA GTTCCCAGTCTGCTAGAGAACTGAAGTCCTGGACAGTCACAGGGTACAGAGACCCAGGCAGCTCCAGCAT CTAGCTGCTTGAGAGACGGTGGCTGGGTGGACCAGCTTGTAGAGTCTCAATGGGCCAATCAAACCGGTGT GCAGGAAGCTGAGCTACTGGCTTCATTCCTGACCACAGCTGAGAAGTATCTGTCTTCCATACATTCTATC TCGAGTTTTGTTTTATTTTTTAAAAATTTTGCTTAAAACAGCATGTTTTATAAAATAAAAGTATTTTTAT CTCATTAGAAAAAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: NM_133992
• Insert Size: 3603 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC075686, AAH75686
• RefSeq Size: 4437 bp
• RefSeq ORF: 3603 bp
• Locus ID: 103135
• UniProt ID: Q8BGF7
• Cytogenetics: 10 D3
• Gene Summary: Catalytic subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein (PABP). PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1 (PubMed:16284618). Also acts as an important regulator of the HIF1A-mediated hypoxic response. Required for HIF1A mRNA stability independent of poly(A) tail length regulation (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and it encodes the longest protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.