Gnat1 (NM_008140) Mouse Untagged Clone

CAT#: MC203321

Gnat1 (untagged) - Mouse guanine nucleotide binding protein, alpha transducing 1 (Gnat1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gnat1
• Synonyms: Gnat-1; Ird1; Ird2; irdc; irdr; Tralpha; transducin
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC058810
  GGCCAGAAGGTCGTCTGAGGAGGCCGGGGAGGCTGCCTACCCTGTCCCTTTGCCTGCTGCTGGGACCATG GGGGCTGGGGCCAGCGCTGAGGAGAAGCACTCCAGAGAGCTGGAGAAGAAGCTGAAAGAGGATGCTGAGA AGGATGCCCGCACTGTGAAACTGCTGCTTCTGGGTGCCGGTGAATCCGGGAAGAGCACTATTGTCAAACA GATGAAGATTATCCACCAGGACGGGTATTCGCTGGAGGAATGCCTCGAGTTCATTGCCATCATCTACGGC AACACTCTGCAGTCCATCCTGGCCATCGTTCGGGCTATGACCACGCTCAACATTCAGTATGGAGATTCAG CCCGGCAGGATGATGCCCGGAAGCTCATGCACATGGCAGATACTATTGAGGAAGGCACAATGCCCAAGGA GATGTCAGACATCATTCAGCGCTTGTGGAAGGACTCGGGTATCCAAGCTTGCTTTGACCGAGCCTCAGAA TACCAGCTCAATGACTCCGCCGGCTACTATCTCTCAGACCTAGAGCGTCTGGTGACTCCAGGATATGTGC CCACTGAGCAGGACGTGTTGCGTTCTCGTGTCAAAACCACTGGTATTATCGAGACTCAATTCTCCTTCAA GGACCTCAACTTCAGAATGTTCGATGTGGGCGGGCAGCGTTCCGAGCGCAAAAAGTGGATCCACTGCTTT GAGGGTGTGACGTGCATCATTTTCATCGCTGCGCTGAGCGCTTACGACATGGTGCTGGTGGAGGACGACG AAGTGAACCGAATGCACGAGAGCCTGCACCTGTTCAACAGCATCTGCAATCATCGCTACTTCGCCACAAC GTCTATCGTGCTCTTCCTCAACAAGAAGGACGTTTTCTCCGAGAAGATAAAAAAGGCACACCTCAGCATC TGCTTCCCCGACTACGATGGACCTAACACTTACGAGGATGCCGGCAACTACATCAAAGTGCAGTTCCTGG AGCTTAACATGCGACGTGATGTGAAGGAGATCTATTCCCACATGACGTGCGCTACCGACACACAGAACGT CAAGTTTGTCTTTGACGCTGTCACCGACATTATCATCAAGGAGAACCTCAAAGACTGCGGGCTCTTCTGA GGTACCTGAATTCAGGTGTGTCTCTTGCCCTGAGATTCTGTAGCCCTGCAGCCCTGTAGCCCTGTAGTCC CCTCAAATACCGTCCTTTCCACAAGCTGCTGCTCTTGTAGCCACACACCCAAAGGCATGAGGCTTGCAGT GCAGACCCTCCCCCAAGTACCCAACTCGACCCTGCTTTTCACCGCCTGGCTGCACTGGCCTCTTGGACCT ACAGCAGCCAGCCTGAGCTAGGACACCTCAGGACTTGGGACAGCTGGAACTCACAGTGACTCAGCAGTGC CCTAGTGACCCGTCTGTCTCCAGCCTCTTCTGCTACCCCCAGCAGCAGCAGCAGCTCACCAGCAGATCCC CATTCCACAAACAGCCCTCCCTCACCAGTTTCCACGAAGGACTTGAGGTATCCCTCCCTCAAGCAGTAGG CCGGTCCAGCCTGCTACCTGCATATGACAAGTCCAACACGTGTCCACCCAGTGGTTAGTGACTGTCCCTC CCAGTAGCCTGAGGACTCCAAAGTCCAAGCTCTGGTTTTTGAAGCCTCGTATAGAGAAGTGAGTGACTTT GACGTGGGTGCCAGGGAAAGAAAGACACAAGCCAAACCCATTCCCGAACATCAGACTCAGGGTTTCTCCA GTCCCTTCTCCCTCTGGGTCTTGCCCCTCTGTCCTTGGACACTTCCACACCACACCTCATCCACCCACAC ACAATACTCAGGGCTGGCTGGTCAAGGTATGCTGTATTGAAGGAGGGTATTATTGACATAGCTATGACCA ATAATGAAGCCATGCTGAGGGGTGGCTCAGAGCAGAGTCCAACCCCTATTGATCAACCATGGGCAAAGAA TATCCCATGTGAGGTTACAGGCAATTCCATGTGACCATGTTGTCCCTCCTGATTCAGGGCCCATTCCTTA GGAGGGAGAACTGAAAACCAGGCCTAAACCATTAGAGACAATGTCCCCCAACCCCCAGCCACTTCTACAA CCTTTGCTTAAGCCTGTGGACCAGAGCCAGTGCTGCTGGAAGAGGCAAGATGCTTCTGGACAGCAAGACT AGGGCACAACCTCTCCCTCCCTTAGCTAGCTGCAGACTCAGCAATAAACCTTTGCACCAGAAAAAAAAAA AAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_008140
• Insert Size: 1053 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC058810, AAH58810
• RefSeq Size: 2245 bp
• RefSeq ORF: 1053 bp
• Locus ID: 14685
• UniProt ID: P20612
• Cytogenetics: 9 58.86 cM
• Gene Summary: Functions as signal transducer for the rod photoreceptor RHO. Required for normal RHO-mediated light perception by the retina (By similarity). Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs), such as the photoreceptor RHO. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Activated RHO promotes GDP release and GTP binding. Signaling is mediated via downstream effector proteins, such as cGMP-phosphodiesterase (By similarity).[UniProtKB/Swiss-Prot Function]