Polr3e (NM_025298) Mouse Untagged Clone

CAT#: MC203212

Polr3e (untagged) - Mouse polymerase (RNA) III (DNA directed) polypeptide E (Polr3e), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Polr3e
• Synonyms: RPC5; Sin
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC037637
  CCACGCGTCCGCCCACGCGTCCGGGCCGCTGCCTGATAGAAGACCCGGCCGCCGCCGTCTCTGCAGCCCG CGGGTCCCTGGGCCGTTCCGCCGCCCGCGCGCGGCCCCGGCGGAGAGATCGAGTTGAGGACTGTGAGGTG GGCTCCCTAGTATGGCCAATGAAGAAGATGACCCAGTCATACAGGAGATCGATGTGTACTTGGCTAAAAG TCTGGCAGAGAAGCTGTATCTCTTTCAGTATCCTGTGCGTCCTGCTTCAATGACCTATGATGACATTCCA CATCTCTCAGCCAAGATCAAGCCCAAACAGCAGAAGGTAGAGCTTGAGATGGCCATCGACACCCTGAATC CCAACTACTGCCGCAGCAAAGGGGAGCAGATCGCACTGAACGTGGACGGTGCCTGTGCTGACGAGACTAG CACATATTCCTCAAAGCTGATGGACAAGCAGACCTTCTGCTCCTCCCAGACCACCAGCAACACAGCTCGT TATGCCGCTGCACTCTACAGGCAAGGTGAGCTCCACCTGACACCTTTACATGGCATCCTACAGCTTCGGC CCAGCTTCTCCTACCTTGATAAGGCAGATGCCAAGCACCGAGAGAGGGAGGCAGCCAATGAGGCAGGGGA TTCTTCTCAGGACGAGGCAGAAGAAGACGTTAAGCAGATCACGGTACGGTTCTCCAGGCCAGAGTCCGAG CAGGCTCGCCAGCGCCGCGTGCAGTCCTATGAGTTCTTGCAGAAGAAGCATGCAGAAGAACCCTGGGTCC ACCTGCACTACTATGGCATGAGGGACAGCCGCTCAGAGCATGAGCGCCAGTACCTGCTGTGTCAGGGATC CAGCGGGGTAGAGAACACGGAGCTCGTCAAGTCACCCAGTGAATACCTCATGATGCTCATGCCGCCCAGC CCAGAGGAGGAGAAGGACAAGCCTGTGGCTCCCAGCAATGTCTTATCTATGGCCCAGCTGCGTACATTGC CTCTGGCTGATCAGATTAAGGTCCTGATGAAGAACGTGAAAGTCATGCCCTTCGCTAATCTCATGAGCCT CCTGGGCCCCTCTGTGGACTCTGTGGCTGTTCTACGTGGCATCCAGAAGGTGGCGATGTTAGTCCAAGGA AACTGGGTGGTAAAGAGTGACATCCTGTATCCCAAGGATTCTTCCAGCCCTCACAGTGGCATGCCTGCTG AGGTGCTCTGCAGGGGCCGAGACTTTGTTATGTGGAAGTTCACACAGAGCCGATGGGTGGTAAGAAAGGA GGTGGCAGCAGTGACCAAACTCTGCGCTGAGGATGTGAAGGACTTTCTGGAGCACATGGCTGTAGTGAGA ATCAACAAAGGCTGGGAGTTCTTACTGCCTTACGACCTGGAGTTTATCAAGAAGCATCCAGATGTGGTCC AGCGGCAGCACATGCTGTGGTCGGGCATCCAGGCCAAATTAGAAAAAGTCTATAATCTCGTAAAAGAAAC CATGCCAAAGAAGCCGGATGGACAATCAGCGCCTGTTGGTCTGGTCTCTGGGGAACAGCGGGTCCAAACA GCCAAAACCAAGGCCCAGCAGAACCATGCCTTCTTGGAGCGTGAACTGCAGCGACGGAAGGAGCAGATGC GGGCAGCCACAGTCCTGCCCAGCGTGCAGATCAAGGAGGAACCCCTGAGTGAGGAGGAAGCCGATGGGGC TGAGCTGGAGGCTGAGGAAGAGGAGCCCATGGACACTGCACCCAGCACTTGCCTCAGCACCAAGTTGGCC AATGGGCTGCCTGCTGGGCGGGCAGTGGGTGGGGACAGCCTAAATGGACACCCAGTTCCAGGCTGTGCCA GCAACCCGGTTGCCTGTGAACTGAAGGCTTTTGTGGAAGCCACCTTTCAGAGACAGTTTGTGCTCACGCT GAGCGAACTCAAGCGCCTCTTCAACCTGCACCTGGCAGGCCTGCCCCCGGGCCATATACTCTTCAGTGGC GTCTCAGACCGAATGCTACAGGATACGGTGCTGGCCGCTGGCTGCAAGCAGATACTGGTGCCTTTTCCCC CACAGACTGCTGCTTCCCCTGATGAGCAGAAGGTATTTGCCCTCTGGGAGTCTGGAGACATGAGTGACCA GCATCGGCAGGTTTTGCTTGAAATATTTTCCAAAAATTACCGAGTACGTCGGAACTTGATCCAGTCTCGG CTGACTCAAGAATGTGGAGAAGAGCTAAGCAAACAGGAGGTGGATAAAGTGCTAAAGGACTGTTGTGTAA GCTGTGGTGGCATGTGGTACCTTAAGGGGACAGTACAATCCTGACAATAGGAGCAAACTGCTAACTCGAC GAATCCAAGCCCAGGGAAGGATGGCAGAGCCACACGTAGTCTTGACTTGCCTCAAGACACAGGAAGAGGA TCTGCCTACCTGGTGGCCTGTGGCGCTGCCTGGCCCAGAGGACAGAGGGGCTCATGCTCAGCCAGTGGCA GGGTCAGCGTAAGTGAGAGCGCTCCCAGCAGAGACCAGCGGGCCTTCTTTGCAGTACAATTTGAAATTCC TGATGTATTTTGCTTATTGGGTTTCATTCTCATAATAAAGAGAGTGTACACTGACATGGGCAGGATTACG AAAATCATGGTTTAATATTTTCCTCTTTTAAATGAATGCCAACATAGTTAAAGATATGTTTACAAGATGA AGAGAACCTTAAGGTTTTTAATATTTAAAATGCCTAGAAGCCAATAATTAGTCTTAGAGTTTCTCTCTGC TAAGACTCCTGCCAATTTTATTAAACCAAATTGAACATTTTACTGTTGGGTTTCTGAGGCCACTTTACCT TTATAGCAGCCTGCTTTCTGTAGCGGTCTCAACTGTTTACACAAACCATAGCAAAACATTAGATTAACTC CATCTTCTCCTAATGTTTGCATAAAGGTGCAAACTAAACCAGGTAAGTGTGGAACAGTATATAAGCGAGG TTATCACTTTGATGTAAAAAAATCTATTTTGTGTATTTTTAAAATAAATGCTAACACTAAAAAAAAAAAA AAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_025298
• Insert Size: 2133 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC037637, AAH37637
• RefSeq Size: 3013 bp
• RefSeq ORF: 2133 bp
• Locus ID: 26939
• UniProt ID: Q9CZT4
• Cytogenetics: 7 F2
• Gene Summary: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific periphjeric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Essential for efficient transcription from both the type 2 VAI and type 3 U6 RNA polymerase III promoters. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts induce type I interferon and NF- Kappa-B through the RIG-I pathway (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.