Paf1 (NM_019458) Mouse Untagged Clone

CAT#: MC203167

Paf1 (untagged) - Mouse Paf1, RNA polymerase II associated factor, homolog (S. cerevisiae) (Paf1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Paf1
• Synonyms: 5730511K23Rik; AA675045
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC029843
  GGGAGCTGCAAGTCTGTGTCTGACGCTGTTTGTGCTCTTACTTCAGTCACACCCGAGCCCTTCTCGTCGG CCCACTTCATCCCCTTTCCCCGCGGAGCCCGGCGTCTACGGGAGCCTGTCCTTGGATCCGGCGTCTCTCA AAGTCGGTACGCCCCTCAGCGTCCGCCTGCGGCCAGCGAGGACGCCTACCGCGCCGCCGTCGCCTGGTCG CGATGGCGCCCACCATCCAGACCCAGGCCCAGAGGGAAGATGGCCACAGGCCCAATTCCCACCGGACCTT GCCTGAGAGGTCTGGAGTAGTCTGCCGAGTCAAGTACTGCAATAGCCTTCCTGACATCCCATTTGACCCC AAGTTCATCACCTACCCCTTCGACCAGAACAGGTTTGTTCAGTACAAAGCAACCTCCTTGGAGAAACAGC ACAAACATGACCTCCTGACTGAGCCAGATCTGGGGGTCACCATTGATCTCATCAACCCTGACACCTACCG CATTGATCCCAATGTTCTTCTGGATCCGGCTGATGAGAAGCTTCTAGAAGAGGAGATTCAGGCTCCCACC AGCTCTAAGAGATCCCAGCAGCATGCAAAGGTGGTACCGTGGATGCGTAAGACAGAGTATATCTCCACTG AGTTCAACAGATATGGCATCTCCAATGAGAAGCCTGAAGTCAAGATTGGGGTTTCTGTGAAACAGCAGTT CACAGAGGAAGAAATATACAAAGACAGGGACAGCCAAATCACAGCCATTGAAAAGACTTTTGAAGATGCC CAGAAATCGATCTCCCAGCATTACAGCAAGCCCCGAGTGACACCAGTGGAGGTCATGCCTGTCTTCCCAG ATTTTAAGATGTGGATAAACCCATGTGCTCAGGTCATTTTTGACTCAGACCCAGCCCCCAAGGACACAAG TGGTGCAGCTGCATTAGAGATGATGTCTCAGGCCATGATCAGGGGCATGATGGATGAGGAAGGGAACCAG TTTGTGGCTTATTTTCTGCCTGTGGAAGAAACACTAAAGAAACGAAAGCGGGACCAAGAGGAAGAGATGG ACTATGCACCAGATGATGTGTATGACTATAAGATTGCTCGGGAATACAACTGGAATGTGAAGAACAAGGC CAGTAAGGGCTATGAGGAGAACTATTTCTTCATCTTCAGAGAGGGTGACGGAGTTTACTACAATGAGCTG GAGACCAGGGTCCGTCTAAGTAAGCGCCGGGCCAAGGCTGGGGTTCAGTCAGGTACCAATGCCTTGCTTG TCGTCAAACACCGTGACATGAATGAGAAGGAATTAGAAGCCCAGGAGGCACGAAAGGCCCAGTTGGAAAA CCACGAACCAGAGGAAGAGGAGGAGGAAGAGATGGAGGCTGAAGAGAAAGAAGCTGGGGGCTCAGATGAG GAACAGGAGAAGGGTAGCAGCAGTGAGAAAGAAGGCAGCGAGGATGAACACTCTGGAAGTGAGAGTGACC GAGAAGAGGGTGACAGAGATGAGGCAAGTGACAAGAGTGGCAGCGGTGAGGATGAAAGCAGCGAGGATGA AGCACGTGCTGCCCGGGACAAAGAGGAGATTTTTGGTAGTGATGCTGATTCAGAAGATGATGCTGACTCT GATGATGAGGACAGAGGGCAGGCCCACAGGGGCAGTGACAATGACTCGGACAGTGGCAGTGATGGTGGTG GCCAGAGGAGCCGCAGCCAGAGCAGGAGTCGTAGCCGGAGTGCCAGCCCCTTCCCCAGTGGCAGTGAGCA CTCAGCTCAGGAAGATGGCAGTGAAGCAGCAGCCTCTGATTCCAGTGAGGCTGACAGTGACAGTGATTGA GCCTCAAGGCTTTCTAATGAGATCAGCACAGACACCATTTTTAGGGCAGGGAAGCACTTTTCTAGTTGTC TGTTTTGTGAGCTTTTGTCACTACCCCTCAATCTTTGCTGTTAATAAACCTAACTTCTCTTGAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_019458
• Insert Size: 1608 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC029843, AAH29843
• RefSeq Size: 2053 bp
• RefSeq ORF: 1608 bp
• Locus ID: 54624
• UniProt ID: Q8K2T8
• Cytogenetics: 7 B1
• Gene Summary: Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. Connects PAF1C with the RNF20/40 E3 ubiquitin-protein ligase complex. Involved in polyadenylation of mRNA precursors (By similarity).[UniProtKB/Swiss-Prot Function]