Pif1 (NM_172453) Mouse Untagged Clone

CAT#: MC203085

Pif1 (untagged) - Mouse PIF1 5'-to-3' DNA helicase homolog (S. cerevisiae) (Pif1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pif1
• Synonyms: 4631410M14; AI449441
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC046611
  CTGAAAGCAACGAGTCTATCGGATCCTTGAGGACAATCGCTCGCGAATCCTAAGTGCATGTGACCCCACC TTCCAGCGCTCCGAGAACTGCAGCAGAGGACGTTTCTCCTCGCTCCAGAGTGCTTGGAATATCTTGGTGG CATCTTCTGTTACCAGAGACAACCTGTTGACACTAAGAGGTCTGGACAGGATTTCCCGTCACCGCAGCCA TACCACCTATTACATCTCGATTTTCTGTGACTTATGCGCTCCGGTCTCTGCACGCCTGCAGAGGCATTGG AGATGCCTTCTAGCACAGAGGCGGCGACCGATGAATGTGACGATGCGGAGCTCCGGTGCCGGGTAGCCGT GGAGGAGCTGAGTCCTGGAGGGCAACCTCGCAAGCGCCAGGCCCTGCGCGCCGCAGAGCTGAGCCTAGGT CGAAACGAACGACGTGAGTTAATGCTGCGACTGCAGGCACCGGGACCCACGGGGCGGCCACGCTGTTTCC CGCTACGCGCCGTGCGCCTCTTCACCCGCTTCGCTGCGACTGGGCGCAGCACGTTGCGGCTCCCCACCGA TGGAGTCCCTGGAGCTGGCTCAGTGCAACTGCTCCTCTCCGACTGTTCCCCGGAGCGCTTGCGCCGATTC CTGCGCACGCTGCGCCTGAAGCTGGCGGTTGCCCCTGGGCCGGGACCCGCCTCTGCCCGCGCACAGTTGC TCGGCCCGCGGCCCCGAGACTTTGTCACCATCAGTCCAGTGCAGCCAGAGGAACTGCAGCGTGCTGCAGC CACCAAGGCTCCAGATTCTGCGCTGGAAAAGCGGCCAATGGAATCCCAGACTAGTACGGAAGCTCCAAGG TGGCCCCTGCCTGTGAAGAAGCTGCGCATGCCCTCCACCAAACCGAAGCTTTCTGAAGAGCAGGCCGCTG TGCTGAGGATGGTTCTGAAAGGCCAGAGCATTTTCTTCACTGGGAGTGCAGGGACAGGAAAGTCCTACCT GCTGAAACATATCCTGGGTTCCCTGCCCCCTACTGGTACTGTGGCCACTGCCAGCACTGGGGTGGCAGCC TGCCACATTGGGGGCACCACCCTTCATGCCTTTGCAGGCATCGGCTCAGGCCAGGCTCCCCTGGCCCAGT GCATGGCCCTGGCCAATCGGCCAGGTGTGCGGCAGGGCTGGCTGAACTGCCAACGTTTGGTCATTGACGA GATCTCCATGGTGGAGGCAGACTTCTTTGACAAGTTGGAAGCTGTGGCCAGAGCTGTCCGGCAACAGAGG AAGCCATTTGGAGGGATCCAGCTCATCATCTGTGGGGACTTCCTACAGTTGCCACCAGTGACCAAAGGCT CCCAGCAGCCTCGGTTCTGCTTTCAGGCCAAGAGCTGGAGGAGGTGTGTGCCCGTGATTCTGGAGCTGAC TGAGGTGTGGAGGCAAGCAGACCAGACCTTCATCTCTCTACTGCAGGCTGTGAGGTTAGGCAGATGTTCA GATGAAGTGACCCGCCAGCTCAGGGCCACAGCTGCCCATAAAGTGGGACGAGATGGAATTGTAGCCACGA GACTATGTACCCATCAGGATGATGTGGCCCTGACCAACGAGAAGCGGCTGAAGGCACTGCCAGGTGATGT ACACAGCTTTGAGGCTATAGACAGTGACCCTGAGCTAAGCCGGACCCTGGATGCTCAGTGCCCTGTTAGC CGTGTCCTTCAGTTAAAGCTGGGGGCTCAGGTCATGCTGGTGAAGAACTTGGCAGTGTCTCGGGGCCTGG TGAACGGTGCCCGAGGGGTGGTAGTTGGGTTTGAGTCCGAAGGGAGAGGGCTCCCCCGGGTACGGTTCCT GTGTGGTATCACTGAGGTCATCCGCACTGACCGCTGGACAGTACAGGTCACTGGGGGACAGTACCTCAGC CGGCAGCAGCTTCCCCTACAGCTGGCCTGGGCCATATCCATCCACAAAAGCCAGGGCATGTCTCTGGACT GTGTGGAGATCTCTCTGGGCCGTGTGTTTGCCAGTGGTCAAGCCTATGTGGCCCTCTCCCGGGCCCGTAG CCTCCAGGGTCTTCGTGTGCTGGACTTCGACCCCACGGTGGTTCGATGTGACTCCCGAGTGCTGCATTTC TATGCCACCCTGCGGCAGGGCAGGGGCCTCAGTCTGGAGTCCCAAGACGATGAGGAGGCAAACTCAGATC TGGAGAACATGGACCCAAATCTCTGACCTCAGCTGAAAGAGAAGACAAACTTTTAGCTTTTTTTCCTGGG TCAAGGCCCTGGGAATTAACTGGGGAGAGGCCTGTGTTTCTTCCCTTATTCAGCCTCTGGTAGGGTTAAG GGACACAGTTTCCCATCTACTTAACTAGCATTGCCTCAGTTTCACCTATCTCCCCGGGGAAATGACTTCC AGGGTTCAAAGCTAGAAATGGTGATGGTTACCAGAGGACAAAGCTCTCTACCAAGGGTGGAACACACAAC CACAGAGTTCTTTGCAGGCTGGAGAGGCAGTGCGGGCAGGGGCTGCATTCAGCAGCAGCAGCAGGAGCAG CCTGTCTTATTACACCGCATTTATTTATTTTGTGTGCTTGTGCACGCACAGCACATTGTACATGTGAAGG TCAGAGGACAACTCGAGGAAGTTGGTTTTCTCTTTCCCCAAGTGTGTTCTGGGGGTTAAATTCAGGTCAC AGGGCTTGGTAGCAGGCACTTATACCCGATGAGCAATCTTGCTACCAGGGTCGGTTCTAATTTTCTTTGT GTTATTATAACAAAATATATAAGGCTGAGTACTTTATAAAAAAAATGATTTATTTTTAATTAATATATGC TCACAGTTCTAGAAGATGAAGAACACGGAACCAGCCTCGTCTCAGCTTTGCTGGGGTTTGACGGTAGCCG GCACCGCCTGGCGGGGAAACTTGCAGGCAGGATCATGAGAGACAGGCAACAGAAGATGGTGATGCTGGGG AAGAGTATTAATCCGTCCATGAGGACAGGACCTCCTTGCTTTAGTTACCTCCCATGCAATCCATCTCTGA AGGGTTACATCATCTTAACCCTGCTACGCTAGGGACTAAGCTTCCAGTACATAAACCTATAAGGGAAACC ATCCAAACTATGGCAGGAGCCTAGAGGGGATTCAGGCCAGACACAAGCCCAAGATAGAAGTTTAATTACC TTCACAGCTGCGCTCAGCCTAGCACAGCCCCAAGTAAACATCATTCAGAGCCCGACTGAGAACAGACGCT GCAAAATGTGCTGGGTTTAGGGGAGAGGCCATGTTTAGGATATGGAGATGGATGTTCTCCGTTGTATTTA TTTAAGCCAAATAAAACTGTGAACCGTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_172453
• Insert Size: 1953 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC046611, AAH46611
• RefSeq Size: 3353 bp
• RefSeq ORF: 1953 bp
• Locus ID: 208084
• UniProt ID: Q80SX8
• Cytogenetics: 9 C
• Gene Summary: DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of both mitochondrial and nuclear genome stability. Efficiently unwinds G-quadruplex (G4) DNA structures and forked RNA-DNA hybrids. Resolves G4 structures, preventing replication pausing and double-strand breaks (DSBs) at G4 motifs. Involved in the maintenance of telomeric DNA. Inhibits telomere elongation, de novo telomere formation and telomere addition to DSBs via catalytic inhibition of telomerase. Reduces the processivity of telomerase by displacing active telomerase from DNA ends. Releases telomerase by unwinding the short telomerase RNA/telomeric DNA hybrid that is the intermediate in the telomerase reaction. Possesses an intrinsic strand annealing activity.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest protein-coding transcript. Variants 1-3 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.