Alg12 (NM_145477) Mouse Untagged Clone

CAT#: MC202989

Alg12 (untagged) - Mouse asparagine-linked glycosylation 12 homolog (yeast, alpha-1,6-mannosyltransferase) (Alg12), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Alg12
• Synonyms: ECM39
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC021379
  CCACGCGTCCGGCGGGGTCCTGCGGAGAGGAGAGGCGCATGAAGTTCAAAGCTCTACATGGCTGGCAAGA AGTCATCGGGCAAGCGGTCATGGCCGCTTCTGGGGCTGCTGGTCACTGTAGCCACCATCCACCTGGTTAT ATGTCCCTACACCAAGGTAGAAGAGAGCTTCAACCTTCAGGCCACACATGACCTCCTGTACCACCAGCTG GACATAGACAAGTACGACCACCACGAGTTTCCTGGAGTTGTTCCTAGGACATTCCTCGGGCCGCTGGTGA TCGCAGCGTTCTCCAGCCCCGTGGTTTATGTGCTCTCGCTTTTAGAAGTATCCAAATTTTATTCTCAGCT GATAGTCAGAGGAGTCCTTGGGCTTGGTGTGATTTCTGGACTCTGGACATTACAAAAGGAAGTGAGACAG CAGTTCGGAGCCACGGTGGCTGTCATGTTCTGCTGGATATCAGCCACACAGTTTCATCTCATGTTCTACT GTACGAGGACACTCCCCAACGTGTTGGCCCTGGCTGTGGTCTTACCAGCCCTCACAGCCTGGCTGCAGCG GAGGTGGGCCCTGTTTGTCTGGCTCTCAGCCTTCGTCATCATTGGCTTCAGGGCTGAGCTGGCCATGCTG CTGGGCATTGCGCTGCTGCTGACCTTGTACCAAAGAAGACTGACGGTGGCCAGAGTGCTCCGACACGCCA TCCCAGCAGGGCTTCTCTGTCTAGGCAAGTTGGCATCTCTGGTTTGCCTCCTGAATAAAAAGTCTTGGCC ATACAAGGTGAGAGCTATGCTGGTGACAGGGCATATCTTGGTGAATGTGGCATATACAGCCACGTCCCTC TATGTGTCTCATTTCAACTACCCTGGTGGGGTTGCGATGCAGCAGCTACATGAGCTGGTACCACCTCAGA CAGATGTCCTTCTTCACATTGATGTGGCTGCTGCCCAGACAGGAGTGTCACGGTTTCTACAGGTCAATGA TGACTGGAGGTATGACAAGAGTGAAGATGTGGGAGCAGCGGCCATGCTGAACTACACCCACATCCTCATG GAGGCAGTGCCTGGGCACCCGGCCCTCTACAGGGACACACATCGAGTCCTGGCCAGTATTGAGGGCACTA CAGGTATAAGCCTGAATTTGATGAAACTGCCACCCTTTGATGTCAACCTGCAGACAAAGCTGGTACTTCT AGAGCGGCTGCTTAGGCCAGCCTGAGAGGACAGGAGATCTCCAGGAACATCGAGTGTGGTCACCTTCTAA TGCAGTGAAGACAATTCAAGAAAACAGCAAAGACCAGGGACTTCCAGCATGTCTACAGCATGCCTGGTGT TAGCCTACATCAGTCTGGGCCAGGCCCTACAATAGCCACACCTAAGGCACAGGGCTGAGAATGTGGAGAA CAGACCACCAAGTGTGATAGTGGCCTGCTCAAACTGTCTGCTATGGTTAGGTGGCCCACAGGATCATTTA CATTCATACTGGGAACCATTCATAACTAAGAACAGTGAGTCAGGCTAGAACACCCTGGCACCCTAGCTCT GCGGTGACCCTCAGTGTATATTTCTTGCCCCTGAAAACCACACTTGTTGCCCATGGCCTCAGGAGACCAC GATATAAACCAGGTCTTTATGGGACAGACAGACACACACACACCACCACCACCACCACCACCACCACCTC CTCCACCACCAATCATGGCAGCTGTTATAGCCCGAGGCATGTTATCTCCATTTCAGATTAACTCTGAGGA CCTACACATGTTCATAAAAGAATGGAAATAAAAATTATGTTTTCTTAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_145477
• Insert Size: 1158 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC021379, AAH21379
• RefSeq Size: 1812 bp
• RefSeq ORF: 1158 bp
• Locus ID: 223774
• UniProt ID: Q8VDB2
• Cytogenetics: 15 E3
• Gene Summary: Adds the eighth mannose residue in an alpha-1,6 linkage onto the dolichol-PP-oligosaccharide precursor (dolichol-PP-Man(7)GlcNAc(2)) required for protein glycosylation.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate splice site and lacks two exons in the central coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1.