Smad2 (NM_010754) Mouse Untagged Clone

CAT#: MC202985

Smad2 (untagged) - Mouse MAD homolog 2 (Drosophila) (Smad2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Smad2
• Synonyms: 7120426M23Rik; Madh2; Madr2; mMad2; Smad-2
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC021342
  CCACGCGTCCGGCGCGCGGCGGTGGAGGAGCAGCTCGCCAAGCCTGCAGCTCGCGAGCGCCGAGCGAGCC TCCCGGAGGGTAGATTTACCGGGCTTTTTCTGAGTGTGGATTGTTACCTTTGGTAAGAAAATGTCGTCCA TCTTGCCATTCACTCCGCCAGTGGTGAAGAGACTTCTGGGATGGAAAAAATCAGCCGGTGGGTCTGGAGG AGCAGGTGGTGGAGAGCAGAATGGACAGGAAGAAAAGTGGTGTGAAAAAGCAGTGAAAAGTCTGGTGAAA AAGCTAAAGAAAACAGGACGGTTAGATGAGCTTGAGAAAGCCATCACCACTCAGAATTGCAATACTAAGT GTGTCACCATACCAAGCACTTGCTCTGAAATTTGGGGACTGAGTACAGCAAATACGGTAGATCAGTGGGA CACAACAGGCCTTTACAGCTTCTCTGAACAAACCAGGTCTCTTGATGGCCGTCTTCAGGTTTCACACCGG AAAGGGTTGCCACATGTTATATATTGCCGGCTCTGGCGCTGGCCGGACCTTCACAGTCATCATGAGCTCA AGGCAATCGAAAACTGCGAATATGCTTTTAATCTGAAAAAAGATGAAGTGTGTGTAAATCCGTACCACTA CCAGAGAGTTGAGACCCCAGTCTTGCCTCCAGTCTTAGTGCCTCGGCACACGGAGATTCTAACAGAACTG CCTCCCCTGGATGACTACACCCACTCCATTCCAGAAAACACAAATTTCCCAGCAGGAATTGAGCCACAGA GTAATTACATCCCAGAAACACCACCACCTGGATATATCAGTGAAGATGGAGAAACAAGTGACCAACAGTT GAACCAAAGTATGGACACAGGCTCTCCGGCTGAACTGTCTCCTACTACTCTCTCTCCTGTTAATCACAGC TTGGATTTGCAGCCAGTTACTTACTCGGAACCTGCATTCTGGTGTTCAATCGCATACTATGAACTAAACC AGAGGGTTGGAGAGACCTTCCATGCGTCACAGCCCTCGCTCACTGTAGACGGCTTCACAGACCCATCAAA CTCGGAGAGGTTCTGCTTAGGCTTGCTCTCCAACGTTAACCGAAATGCCACTGTAGAAATGACAAGAAGA CATATAGGAAGGGGAGTGCGCTTGTATTACATAGGTGGGGAAGTGTTTGCTGAGTGCCTAAGTGATAGTG CAATCTTTGTGCAGAGCCCCAACTGTAACCAGAGATACGGCTGGCACCCTGCAACAGTGTGTAAGATCCC ACCAGGCTGTAACCTGAAGATCTTCAACAACCAAGAATTTGCTGCTCTTCTGGCTCAGTCTGTCAACCAG GGTTTTGAAGCCGTTTATCAGCTAACCCGAATGTGCACCATAAGAATGAGTTTTGTGAAGGGCTGGGGAG CAGAATATCGGAGGCAGACAGTAACAAGTACTCCTTGCTGGATTGAACTTCATCTGAATGGCCCTCTGCA GTGGCTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGTGCGATGCTCAAGCATGTCGTAAACCCAT CAAAGACTCGCTGTAACAGCTCCTCCGTCGTAGTATTCATGTATGATCCCGTGGACTGTTTGCTATCCAA AAATTCCAGAGCAAAAACAGCACTTGAGGTCTCATCAGTTAAAGCACCTTGTGGAATCTGTTTCCTATAT TTGAATATTAGATGGGAAAATTAGTGTCTAGAAATGCCCTCCCCAGCGGGGAAAAAGAAGACTTAAAGAC TTAATGATGTCTTGTTGGGCATAAGACAGTATCCCAAAGGTTATTAATAACAGTAGTAGTTGTGTACAGG TAATGTGTCCAGACCCAGTATTGCAGTACTATGCTGTTTGTATACATTCTTAGTTTGCATAAATGAGGTG TGTGTGCTGCTTCTTGGTCTAGGCAAGCCTTTATAAAATTACAGTATCTAATCTGTTATTCCCACTTCTC CGTTATTTTTGTGTCTTTTTTAATATATAATATATATATATCAAGATTTTCAAATTATCATTTAGAAGCA GATTTTCCTTGTAGAAACTAATTTTTCTGCCTTTTACCAAAAATAAACAAACTCTTGGGGGAAGACAAGT GGATTAACTTGGAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_010754
• Insert Size: 1404 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC021342, AAH21342
• RefSeq Size: 2133 bp
• RefSeq ORF: 1404 bp
• Locus ID: 17126
• UniProt ID: Q62432
• Cytogenetics: 18 51.42 cM
• Gene Summary: Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (1). Both variants 1 and 2 encode the same isoform. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.