Cpsf6 (NM_001013391) Mouse Untagged Clone

CAT#: MC202653

Cpsf6 (untagged) - Mouse cleavage and polyadenylation specific factor 6 (Cpsf6), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cpsf6
• Synonyms: 4733401N12Rik; AI256641; CFIM; CFIM68; HPBRII-4; HPBRII-7
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC068133 sequence for NM_001013391
  GCGGGCCGACCTGCAGGAGGCGGCGGCGGCGGCGGCCGAGGCCGAGGGAAGATGGCGGACGGTGTGGACC ACATAGACATTTACGCGGATGTGGGTGAAGAGTTCAACCAGGAAGCAGAATATGGTGGACATGATCAGAT AGATTTGTATGATGATGTCATCTCTCCATCTGCAAATAATGGCGATGCCCCAGAAGATCGCGACTACATG GATACTCTTCCACCAACTGTTGGTGATGATGTGGGTAAAGGAGCCGCACCAAACGTTGTGTACACTTACA CTGGGAAGAGAATCGCATTGTATATTGGAAATCTAACATGGTGGACAACAGATGAGGACCTAACTGAGGC CGTTCATTCTTTGGGAGTAAATGATATTTTGGAGATAAAATTCTTTGAAAATCGGGCAAATGGACAATCA AAGGGATTTGCCCTTGTTGGTGTTGGATCTGAAGCATCTTCCAAAAAGTTAATGGATCTTTTGCCTAAAA GAGAACTTCATGGTCAGAGTCCTGTTGTAACTCCATGCAATAAGCAGTTCCTGAGTCAATTTGAAATGCA ATCCAGGAAAACTACACAGTCAGGACAGATGTCTGGGGAAGGGAAAGCCGGTCCTCCAGGAGGCGGTTCA CGCGCAGCGTTTCCTCAAGGTGGTAGAGGACGGGGCCGGTTTCCAGGGGCTGTTCCTGGTGGGGACAGAT TTCCTGGGCCAGCAGGACCAGGAGGGCCACCTCCACCTTTTCCAGCTGGACAAACTCCACCACGTCCACC TTTAGGCCCACCTGGCCCACCTGGTCCACCAGGTCCTCCACCTCCTGGTCAGGTTCTGCCACCTCCTCTA GCAGGACCTCCTAACCGAGGAGACCGCCCTCCACCACCAGTTCTTTTTCCTGGACAACCTTTTGGGCAGC CTCCGCTGGGTCCACTTCCTCCTGGGCCTCCACCTCCAGTTCCAGGCTACGGCCCCCCTCCAGGTCCACC GCCTCCACAGCAGGGACCACCTCCACCTCCAGGCCCCTTTCCACCTCGCCCACCAGGTCCACTAGGGCCT CCCCTCACACTTGCTCCTCCTCCACATCTTCCGGGACCACCTCCAGGTGCCCCGCCACCAGCTCCACATG TGAATCCTGCTTTTTTTCCTCCACCAACTAATAGTGGCATGCCAACATCAGATAGTCGAGGTCCACCACC AACAGACCCATATGGCCGACCTCCCCCATATGATAGGGGTGACTACGGTCCCCCTGGGAGGGAAATGGAT ACTGCAAGAACACCTCTGAGTGAAGCTGAGTTTGAAGAAATCATGAATAGAAATCGGGCAATCTCAAGCA GTGCTATTTCAAGAGCTGTGTCTGACGCTAGTGCTGGTGATTATGGGAGTGCTATTGAAACATTGGTAAC AGCAATTTCTTTAATTAAACAATCCAAAGTCTCTGCAGATGATCGTTGCAAAGTTCTTATTAGCTCTCTG CAGGATTGCCTTCATGGAATCGAGTCCAAGTCCTATGGGTCTGGATCCAGACGTGAACGATCAAGAGAAC GGGACCATAGTAGATCACGGGAAAAGAGTCGTCGCCATAAATCTCGGAGTAGAGATCGCCACGATGACTA TTACAGAGAGAGAAGCAGAGAACGAGAGAGACACCGGGATCGGGACCGCGACCGTGACCGAGAGCGTGAC CGAGAGCGAGAATACCGTCATCGTTAGAAGCTGAAGGAAGAGGAACACCTTCCAAGACAAAAGAGTCTTC TCGGGGGAAAAGTGACGCTTGTCCAGCAGTTTGCTTCTTGTGATTGAACTGAACCTGTAAGGCTTCATGG ATAAACTGAACAGGAATAGATCTGAATAAAGCAAATCTGCATAAATGGTAACCAGTAGCTCTACTTTTAT TTTTTATGTTGCTTAACTGTTTTATTTGAAGGAAACCTGTGTGATTTAAAAAGTTAATAGCTTTTGCAAC TTTATTACTGGTTATATATATTTGGCCATTATAATGTGCAAGCAATTGGAAAAAAGTCAAGTAAATGCTT GTTTTTATAGTAGTTTGTTCTTGTTAAAATGTTCCTATGATAATGTCTGTAAACAGCACCGATTTGATTA CACTAGATGTAGTGTTGTAATAAACTGTTTATGGGGCTGATGTGTAAAGCTGTTCACGTTATTTGATGTT TACACCTCAGGGAAAGGCTTGTGTTCAGCAATATCTAAAGATAATGTTACTATGACAACATTTTTACTGT CCTTTAAAAAGCATTGCAATAGCGTGTTTGGATATGCATCAATCTAATCTTGCGTTCAGTGAATTAAACA TGACTAATTAAGTGTCTATTGCCCTTGATTTTGATATTAGAATAGGTGACTACATGGGTATTTAATATTT CTATATTCTGCTTTTCTAGCTGTTTTTACCTAGTTAGCTTGTGATTTTGCTGAATGGTATGTAAACGTGT AAAAACTGAAATTTGACAGAGCAATCCAGTCAATGTGTTAGGGGTCAAAAAAATTGAAGGCTGTAACATT TTTGGTAAAAACTCATGTATTTGCTACAGTAGTGCAGCTTAATTACAACCCAGATATACTGAAATAATAA ATTGTGAACTGCTAGCATTTAAGATAGATTTTGATAATTCTGGTTTCATGCAGTAATTTACTGTTTGATG CTGATGGTGCCATAGGCAGTGTAATTATGGCTATACAGCAAGATGCGTAAAGAAAGCTCTAGTATTGTAC TTGATTCAGATTTCTGAAGGGTGTGATGTTTATTAACATTGGGCTGTATTGTTCAGTGTGAATAATGAAA ATTTATAATTACACTGTCTTCATGGCTCAAGGAATTGTGTGTGTATTCCTGTAACCTTGTTGATCTATGC TTGATTAGAGTTAATCAGCTGATTTTTTTTTCTTTTTTTTTCCTTTCTTTCATTTTTTTTTTTAAAAGAT CCAGCCTTAATAAAAGGTTGTATTAAAAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AA
• Restriction Sites: AscI-NotI
• ACCN: NM_001013391
• Insert Size: 1656 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC068133, AAH68133
• RefSeq Size: 3012 bp
• RefSeq ORF: 1656 bp
• Locus ID: 432508
• UniProt ID: Q6NVF9
• Cytogenetics: 10 D2
• Gene Summary: Component of the cleavage factor Im (CFIm) complex that functions as an activator of the pre-mRNA 3'-end cleavage and polyadenylation processing required for the maturation of pre-mRNA into functional mRNAs. CFIm contributes to the recruitment of multiprotein complexes on specific sequences on the pre-mRNA 3'-end, so called cleavage and polyadenylation signals (pA signals). Most pre-mRNAs contain multiple pA signals, resulting in alternative cleavage and polyadenylation (APA) producing mRNAs with variable 3'-end formation. The CFIm complex acts as a key regulator of cleavage and polyadenylation site choice during APA through its binding to 5'-UGUA-3' elements localized in the 3'-untranslated region (UTR) for a huge number of pre-mRNAs. CPSF6 enhances NUDT21/CPSF5 binding to 5'-UGUA-3' elements localized upstream of pA signals and promotes RNA looping, and hence activates directly the mRNA 3'-processing machinery. Plays a role in mRNA export.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.