Nploc4 (NM_199469) Mouse Untagged Clone

CAT#: MC202520

Nploc4 (untagged) - Mouse nuclear protein localization 4 homolog (S. cerevisiae) (Nploc4), transcript variant B, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nploc4
• Synonyms: AK129375; mKIAA1499; Npl4
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC065156 sequence for NM_199469
  GGCACTGGCGGAGCCGGGCGGCGAAGCTGGAGCGGCGGTGGCGGCGGCGGCGGGAGGCCGGGGCCGGGGC TGAGGCCGAGGCCGGGGCTGCGAGGCCCTTGGGGAGACAGGCGGCGGGAGCCCGGGGCCCGAGCCCGGAG ACTGGAGCGGCCGCCATGGCCGAGAGCATCATAATCCGTGTCCAGTCCCCTGATGGCGTGAAGAGGATCA CAGCTACGAAGAGAGAGACGGCTGCCACCTTCCTGAAAAAGGTTGCAAAGGAGTTTGGCTTCCAAAACAA CGGCTTCTCAGTTTACATCAATAGAAACAAGACTGGAGAGATAACAGCATCGTCCAGCAAATCCCTCCAT TTGCTAAAAATCAAGCATGGCGATTTGTTGTTCCTGTTTCCCTCAAGCCTTGCTGGACCCTCGTCTGAAA TGGAAACATCGACCTCGGTAGGGTTAAAGGCCTTTGGTGCTCCTAACGTGGTTGAAGATGAGATCGATCA ATACCTCAGCAAGCAGGATGGGAAGATTTACCGGAGCCGAGACCCACAGCTATGCCGCCATGGTCCCTTG GGCAAGTGTGTGCATTGTGTCCCACTGGAGCCATTTGATGAGGACTATCTGAACCACTTGGAGCCTCCTG TGAAGCACATGTCCTTCCATGCCTACATCCGGAAGCTGACTGGAGGGGCTGACAAGGGTAAGTTTGTTGC CCTGGAGAACATCAGCTGCAAGATTAAGTCAGGGTGTGAGGGACACCTCCCATGGCCAAATGGTATCTGC ACCAAATGCCAGCCCAGTGCCATCACGCTGAACAGACAGAAATACCGACACGTGGACAACATCATGTTTG AGAATCATACTGTTGCTGACCGCTTCCTTGACTTCTGGAGAAAGACCGGAAACCAGCATTTTGGGTACTT ATACGGACGGTACACAGAGCACAAGGACATTCCCCTCGGCATCAGGGCTGAAGTGGCTGCAATTTACGAG CCTCCTCAGATTGGTACACAGAACAGTCTGGAGCTTCTGGAAGACCCGAAAGCTGAGGTGGTTGACGAGA TTGCCGCCAAACTTGGCCTGAGGAAGGTTGGATGGATATTCACGGACCTTGTCTCAGAAGACACCCGAAA GGGTACAGTCCGATACAGTCGGAATAAGGACACCTATTTCCTGAGTTCAGAAGAATGCATCACTGCAGGA GACTTTCAAAACAAGCACCCCAACATTTGCCGGCTCTCTCCCGATGGTCATTTTGGATCCAAGTTTGTTA CTGCAGTGGCCACAGGTGGTCCTGACAACCAGGTTCACTTTGAAGGGTACCAGGTATCCAATCAGTGCAT GGCACTAGTCCGAGATGAGTGTCTTCTACCTTGTAAGGATGCCCCTGAGCTTGGCTATGCTAAGGAGTCC AGCAGTGAGCAGTATGTTCCTGATGTGTTTTATAAGGACATAGACAAGTTTGGCAATGAGATCACCCAGC TGGCCCGTCCCCTGCCTGTGGAGTATCTCATCATAGATGACTTCCATAGTTTGGCCACCTACTTGTCCCA GAATACCTCATCCGTGTTCTTGGATACCATTTCCGACTTCCATCTCCTGCTGTTTCTGGTCACCAATGAA GTCATGCCGCTGCAGGACAGTATCAGCCTGCTGCTAGAAGCCGTGAGGACCAGAAATGAGGAGCTAGCAC AGACATGGAAAAAGTCTGAACAGTGGGCCACCATTGAGCAGTTATGCAGCACCGTTGGTGTGCAGCTCCC AGGCCTCCACGAGTTTGGTGCAGTCGGGGGCTCTGCACGCGCTGCTACCTCTGCCATGTGGGCCTGTCAG CACTGCACCTTCATGAACCAACCTGGCACAGGCCACTGCGAGATGTGCAGCCTCCCCCGGACCTAGGGCT CTGGGCCTCTGCTGCATGGGACTGGGCCAGCCCAGCCCTCTGAAGTCTGGAGCGCCACATGTTCCCTATA AGGCCTGAAGCCCACAGCCCTGAAACAGTGGGGCCAGCTGCCCTGGTATTCCAGATACCCTGAAGCGTCT CCGTGCAGATCTTGAAGAGAAAGAACCTCGGCTGCTGTGCTGGGGTTGAACAGAGCCTCCCACCTGGGAA GGCAGAGCAGACCTGCCCAAGAGCTCCCCACACGCCTCACGTGTTGGAGGGACTGAACACGTCCAGCCAA TCACCTGAAATTGGGGTGTACAGAGAGGGTGCTGCTGTCACCCAGCTATCTCTCCTTCCTCTATGATCTT TGTTTTCTGCCTTCCCACCTTCTCCCCCAGTGTTCGGATGAATGTTCCTGGCTGTCTCTGTTCCCTTGCT GTGGGGCTGTGTCCCTCCTTTGGGAGCATGTGTGGCTAGGGTGTGGCAGCCTTTGTCTGACATGCAGTAT AGTAGGTCCCTGACCTTTACTTGCCTGAAATCAGCAGTTATCAATAATTCCCACCCAGGAAACTTTTATT TATTGTTTTTTAAAGATAAAACTGCACAGATGGGAGTGAGAGATTTACCTCTGCTCCTTCCTCCTTCAGT GCAGCCCCTCTTGGGTGGTTTATTGGGCAGGAGGCCACCATCATTTCTCCTCAGAAGCAAACCTCAGTGC CTGAGACTTTCCTGCCACGCTGCAGACAGTGGCAGTTGGTAGCATGGGTGGTTCATCAGAGCAGTGGTGG CTCTTGCCTGGCAGTCAGCCCCTTCAAGTCTGGACAGCCCTGGAGTTCTGGTCCCCTCTCCTAGCACTGT ACCTTTACCCAGCGAGTCCCCACACTGTGGTCTTCTTGCACAGTGTGTGGCAGTTGAGAAAGCCTAGAAA AGGTGCCCATTTGACCACTGTGAGGCAAATAGAAACAGTCCCTAAGGGTCCTATGTGGCTGTGTTGTGAC AGCCCTTACCCAGGTAAGAGATGGATGTCGTGATGCCTCGTTGCCTTAATGAGAGCTGAGTCAGCTGTCC TTCACCCTGGGAAAGTCCATGCCAGCTGTGGACTGCTGGGAGCCATGTTTGTCACCTTAGTAGAATGCAC CAATGTTCCCTTCTGCATCTCTGGCCCTGTGGACAGAACACTTATTGCTAGGTTATCTATCGTGTGGCAG GGCTGGCCAGCACCGTTGGGTCTCACCTGGCATGAGATCTGAAGCTAAAGCCCTGGGACTGTGGAGCCAT TGGGCTTGGTTTGGTATTGACAGGGTCATCCTGAGGGGTTAGCAGTGGTAGAGAGCAAGGCAAGTGATCT CTCCAGCTACCTGCCAGCAGTAGAGAGAGAGAGAGAGAGTGCTGAGGGCTGGTAGTGTCTCTGGCTAGGG CTTCACCTTTCGGTTTTTTGTTTTTTGTTTTTTCTGGTCCCTGAGTCTGGCATTTCTGTCTGTGACTTAG CTATGTTTCTGATGCCAATCACACTAGCACCATTACATGCTGGGCAGGTGCTGGGCAGCACAGACGCTGG AGACCTGTTTGCCACTGCTTGTCCCCCATTCATCTTTGTCTACAAAGTTAATTACGTTCTCATGTTCTGG CATCCACATAATTCTTGTTTTCTCTGACTTATAGCTAAAGAAATTTTTGTGGTTTCTCTGCTTATGGTTT TGAGTTACTCTTTGTTCAGTAATGCACTTTATTTTATTGTCCAAAGAGAGTCAAGCTAAAGCTACCACCT TGGGGTGAGCCCTACAGAAGGTTCAGACCCGGGCAGCTCAGCTACCTCCTTCTACATGGAGGTGGCCCTG GTCCCACCTCTAATCCCTACTGTCATCAGGCACTACAGCCCAAAAAGAGGTGCTTGTGAGCACACCCACC CAAGTGAACCCAGTGGCTGCCATGGTCCTCTTCAGGCTCAGAGATGGGTCTAGCCCTGGCCTTGCTCTGA TCAGCCTGCTCCTGGGGCATGGGTGCTCCCGCTTCTGCTCTGCTTGGGCTCACAGGTATGCACTGGCTGG GTGTGGATGAGTATTTTTCTGACCCCAGCCCTGCAGTGGAACCTAATAAAAGCACCTGAAGCGAAAAAAA AAAAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: NM_199469
• Insert Size: 1731 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC065156, AAH65156
• RefSeq Size: 4008 bp
• RefSeq ORF: 1731 bp
• Locus ID: 217365
• UniProt ID: P60670
• Cytogenetics: 11 E2
• Gene Summary: The ternary complex containing UFD1, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope (By similarity). Acts as a negative regulator of type I interferon production via the complex formed with VCP and UFD1, which binds to DDX58/RIG-I and recruits RNF125 to promote ubiquitination and degradation of DDX58/RIG-I (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (B) lacks an in-frame exon in the coding region, compared to variant A. This results in a shorter protein (isoform B), compared to isoform A. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.