Brpf1 (NM_030178) Mouse Untagged Clone

CAT#: MC202327

Brpf1 (untagged) - Mouse bromodomain and PHD finger containing, 1 (Brpf1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Brpf1
• Synonyms: 4833438B11Rik; 4930540D11Rik; Brpf2
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC046521 sequence for NM_030178
  CTGCTGCTGCCGCCGCTGCCACAGCCTTTGCCGCCACGGTCTCGCCGCTGCTGTTCCTGTGCCGCCTCCA GGAGCCCCCCCATTGTCCCGGCGGCACCGGGTTCCCAGGCCAGAAGATCCAAAAGCTCGGGAAACGCGAA GATGTGACAGCATGGGGGTGGACTTTGACGTGAAGACCTTCTGCCACAATTTGCGGGCAACTAAGCCACC ATATGAGTGCCCTGTGGAGACTTGCCGCAAGGTTTACAAGAGTTACAGTGGTATCGAGTACCACCTGTAC CACTATGACCACGACAGCCCACCACCCCCACAGCAGACCCCACTGCGCAAGCACAAAAAGAAAGGGCGCC AGTCACGACCAGCCAACAAGCAGTCACCCAGCCCCTCTGAAGTCTCACAGTCACCAGGCCGAGAGGTGAT GAGCTATGCTCAGGCCCAGCGCATGGTAGAAGTGGACCTTCATGGCCGTGTCCACCGAATCAGCATCTTT GACAACTTGGATGTGGTGTCAGAGGATGAGGAGGCCCCTGAGGAGGCCCCTGAGAATGGCAGCAACAAGG AAAACACTGAGACACCTGCGGCTACACCTAAGTCAGGCAAGCATAAGAACAAGGAGAAACGAAAAGACTC TAACCACCACCATCACAGCGCTCCTGCCAGTGCTGCTCCCAAATTGCCTGAGGTGGTGTATCGTGAGCTA GAGCAAGATACCCCTGACGCACCACCCCGGCCCACTTCCTACTACCGGTACATCGAGAAATCTGCAGAGG AGCTGGATGAGGAGGTGGAGTATGACATGGATGAAGAGGACTATATCTGGCTGGATATCATGAATGAGCG GCGGAAGACTGAGGGTGTAAGTCCCATCCCACAAGAGATCTTTGAGTACTTAATGGACCGTTTGGAGAAG GAGTCGTACTTTGAGAGTCACAATAAAGGTGACCCCAATGCACTAGTGGATGAAGATGCCGTGTGCTGTA TCTGCAATGATGGCGAGTGCCAGAACAGCAATGTTATCCTCTTCTGTGACATGTGTAACTTGGCTGTGCA CCAGGAGTGCTACGGTGTCCCCTATATCCCTGAAGGCCAGTGGCTGTGCCGCCGTTGCCTGCAGTCACCT TCTCGTGCAGTGGATTGTGCTCTGTGCCCCAATAAGGGTGGTGCCTTCAAGCAGACAGATGATGGCCGCT GGGCCCACGTGGTGTGTGCCTTGTGGATCCCTGAGGTTTGCTTTGCCAACACAGTCTTCCTAGAACCTAT TGACAGCATTGAGCACATCCCACCAGCTCGGTGGAAGCTCACCTGCTACATTTGTAAACAGCGGGGCTCT GGAGCCTGCATCCAGTGCCATAAGGCCAATTGCTACACAGCCTTCCATGTGACATGTGCCCAACAAGCTG GCCTTTACATGAAGATGGAACCTGTGCGGGAGACAGGTGCCAATGGTACCTCCTTTAGCGTCCGCAAGAC AGCCTACTGTGACATCCACACACCCCCAGGTTCTGCTCGTCGCCTGCCTGCCCTATCCCACAGTGAGGGT GAGGAAGAAGAGGATGAAGAAGAAGATGAGGGTAAAAGCTGGAGCTCAGAGAAGGTCAAGAAGGCCAAGG CCAAGTCTCGGATTAAGATGAAGAAAGCTCGGAAGATCTTGGCAGAGAAGAGGGCAGCAGCACCTGTGGT GTCCGTGCCCTGCATCCCGCCACACAGGCTCAGTAAGATCACCAACCGCCTGACCATCCAGAGGAAGAGC CAGTTCATGCAGAGGCTGCACAGCTACTGGACTCTGAAACGACAATCACGGAATGGGGTCCCACTACTCA GGCGCCTACAAACACACCTTCAGTCTCAGAGGAACTGTGAACAAGTTGGGAGAGATTCTGATGATAAAAA CTGGGCCCTCAAAGAACAGCTCAAGTCCTGGCAGAGACTGCGGCATGACCTGGAGCGAGCTCGGCTGCTG GTGGAGCTGATCCGCAAGCGAGAGAAACTAAAAAGGGAGACGATCAAGATCCAGCAGATTGCCATGGAGA TGCAGCTGACCCCTTTCCTCATCCTCCTCCGAAAAACCTTGGAGCAGCTCCAAGAGAAGGACACAGGCAA CATCTTCAGCGAGCCGGTCCCTCTGTCTGAGGTACCCGACTACCTAGACCACATCAAAAAGCCCATGGAC TTTTTCACCATGAAGCAGAACTTGGAGGCTTACCGCTACTTGAACTTTGATGATTTTGAGGAGGACTTCA ACCTCATTGTCAGCAACTGCCTAAAGTATAATGCCAAGGACACCATCTTCTACAGGGCAGCAGTGCGACT CCGTGAGCAGGGTGGTGCTGTGCTCCGTCAAGCCCGGCGACAGGCAGAAAAAATGGGCATTGACTTTGAG ACGGGCATGCATATCCCTCACAACCTGGCCGGAGATGAGGTCTCACACCACACTGAAGATGTAGAGGAAG AACGGCTGGTCCTGCTGGAGAACCAGAAACACCTGCCAGTAGAAGAACAGCTGAAGTTGTTGCTGGAGAG GCTGGATGAAGTCAATGCCAGCAAGCAGAGTGTGGGCCGCTCTCGGCGTGCAAAAATGATCAAGAAGGAG ATGACAGCATTGCGGCGGAAGCTTGCTCACCAGCGGGAGACTGGCCGGGATGGGCCTGAGCGTCATGGCC CCTCAGGTCGGGGCAATCTGACACCCCACCCAGCAGCCTGTGACAAAGATGGACAGACCGACAGTGCTGC AGAAGAGAGCAGCAGCCAGGAAACAAGCAAAGGCCTGGGTCCCAACATGTCCTCAACCCCCGCACATGAG GTGGGCAGGAGAACCTCAGTTCTGTTCTCCAAAAAGAACCCGAAGACAGCTGGACCGCCCAAGAGGCCGG GCCGGCCCCCAAAAAACCGGGAGAGCCAGATGACCCCCAGCCACGGAGGCAGTCCTGTGGGGCCCCCTCA GCTCCCCATCATGGGCTCCCTACGTCAGCGCAAGCGGGGTAGGAGCCCCCGGCCCAGTTCAAGCTCAGAC AGCGACAGTGATAAGTCCACAGAAGATCCCCCAATGGACTTACCAGCCAATGGCTTCAGCAGTGGGAACC AGCCAGTGAAGAAGAGTTTCTTGGTGTACCGTAATGACTGCAACCTTCCCCGAAGCAGCTCAGACTCTGA GTCCAGCAGCAGCAGCAGCAGCAGTGCAGCCTCAGACCGGACCAGCACAACTCCCTCAAAACAAGGCAGG GGCAAGCCCTCTTTCTCTCGGGGCACATTCCCAGAAGACAGTAGTGAAGATACCTCAGGCACTGAGAATG AGGCCTACTCCGTGGGCACTGGCCGCGGCGTGGGCCACAGCAGTAAGTACCCTCACCCAAAGTCAGGGGT GCTGGGGACCCAGTTTCAAGGCCTTGCCAGCCCCCCAGCTGCTGATCCGCCCCCTCTCTCCCGTTCCTGT GAAGTGGTAAGAAAGAGTCTGGGTCGAGGAGCTGGCTGGCTGTCAGAGGATGAGGACTCCCCGTTGGATG CTCTGGACCTCGTGTGGGCCAAATGCCGAGGCTATCCATCATACCCAGCTCTGATCATTGATCCAAAGAT GCCCCGAGAAGGTATGTTCCACCATGGGGTTCCTATCCCTGTACCACCACTGGAGGTTCTAAAACTTGGG GAACAAATGACACAGGAAGCCCGAGAGCATCTCTACCTCGTTCTCTTCTTTGACAACAAACGAACCTGGC AGTGGCTGCCCCGGACTAAGCTTGTTCCTCTGGGTGTGAACCAGGATCTAGACAAAGAGAAGATGCTGGA GGGCCGCAAGTCCAACATCCGCAAGTCAGTGCAGATTGCTTACCACAGGGCTCTGCAGCACCGAAGCAAG GTGCAGGGTGAGCAGAGCAGCGAGACCAGCGATAGTGACTGATACTCTCAGAGCCCGGTGGTGCTGTGAT GGCTCTCCTTCCCTTTGCTCATTGTCCTGGAGTGATGCCGCCCTCTGCACTGATTAATTCCTGGTCTTGG GGCCAGTCTCAGGGGAAGTTGGGTAGGGGAGGTCCCTCCTGCCCTGAGTGCAGCTGGACTGTACAAAACA CTCCAAGGGCCCATGGCCACTCAGTGCAAGGAGAGGAGAGGTCTCAGAGGTCTAACGAGTTCAAAAACTC ATGGCATTGTAGGGCAGGGTGGTGGGCCACAGTAAGAATACTGTAAAAAACAGGCCCTACCACCCTTACC TACTCATGCCAGGGGAATTCATACCTGCCTAGAGGCCTGAGGCCCTTGCAGGTGGTAAGGTAAATCAGCA TCATCCCAGCCTAGCCGTGGGATTGATGTCTGTCTAGCCTGGAAGAGGGGCACTAGGTGACCCCTCCCCT GCTGTTGTAAATACTGTAATTATTGGAGAATTTAAATTATTCTCATTTGTAACTGCGTTTCCGGGTCGCG CCAGAGTCATTTGGTACTAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_030178
• Insert Size: 3741 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC046521, AAH46521
• RefSeq Size: 4443 bp
• RefSeq ORF: 3741 bp
• Locus ID: 78783
• Cytogenetics: 6 E3
• Gene Summary: Component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity. Preferentially mediates histone H3-K23 acetylation (PubMed:27939640). Positively regulates the transcription of RUNX1 and RUNX2 (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR and uses an alternate in-frame splice site in the central coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1.