Pias4 (NM_021501) Mouse Untagged Clone

CAT#: MC202219

Pias4 (untagged) - Mouse protein inhibitor of activated STAT 4 (Pias4), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pias4
• Synonyms: Pias-gamma; Piasg; PIASY
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC025159 sequence for NM_021501
  GTGGCCAAGATGGCGGCAGAGCTGGTGGAGGCCAAAAACATGGTGATGAGCTTCCGAGTATCAGACCTGC AGATGCTGCTGGGCTTTGTAGGCCGGAGCAAGAGTGGGCTGAAGCACGAACTGGTGACCAGGGCCTTGCA GCTGGTGCAGTTCGACTGTAGCCCAGAGCTTTTCAAGAAGATCAAAGAGCTGTATGAGACTCGCTATGCC AAGAAGAGTGCAGAGCCCGGCCCACAGGCACCACGGCCCCTGGATCCCCTGGCACTACACTCCATGCCCA GGACTCCCCTGTCAGGGCCCACCGTGGACTACCCCGTGCTGTACGGGAAGTACTTGAATGGACTGGGGCG CCTGCCCACCAAGACCCTTAAGCCGGAGGTACGCCTGGTTAAGCTCCCCTTCTTCAACATGCTGGACGAA TTGCTGAAGCCCACTGAACTGGTCCCACAGAGTGCAGAGAAGCTGCAAGAGAGCCCATGCATCTTCGCGC TAACACCTCGGCAGGTGGAGATGATCCGCAACTCCAGGGAGCTGCAGCCTGGAGTGAAAGCTGTGCAGGT GGTGCTGAGGATCTGTTACTCTGATACCAGCTGTCCGCAGGAGGACCAATACCCACCCAACATCGCTGTG AAGGTCAACCACAGCTACTGCTCAGTGCCGGGCTACTATCCCTCCAACAAGCCTGGTGTGGAACCTAAGA GGCCCTGCCGCCCCATCAACCTTACCCACCTCATGTATCTGTCCTCGGCCACCAACCGCATTACTGTCAC CTGGGGCAACTATGGCAAGAGCTACTCTGTGGCTTTGTACCTGGTGCGACAGCTGACCTCCTCAGACCTG CTACAGAGGTTGAAGACGATTGGGGTGAAGCATCCTGAGCTGTGCAAGGCTTTGGTCAAGGAGAAGCTTC GCCTAGACCCCGACAGCGAGATCGCCACTACCGGAGTGCGAGTGTCCCTCATCTGCCCACTGGTGAAGAT GCGCCTCTCGGTGCCCTGCCGTGCAGAGACCTGCGCACACCTGCAGTGCTTTGATGCTGTGTTCTACCTG CAAATGAATGAGAAGAAGCCCACCTGGATGTGCCCTGTGTGTGACAAGCCTGCAGCCTATGACCAGCTCA TCATAGATGGGCTGCTGTCGAAGATCCTGAGTGAGTGTGAGGGCGCGGATGAGATTGAGTTCCTGGCGGA AGGCTCATGGCGTCCAATCCGTGCAGAGAAGGAGCCAAGCTGCAGCCCACAGGGCCCCATCCTGGTGCTA GGCACCTCGGACGCCAATGGGCTCGCACCAGCCTCCAGCACCCCCGGTATAGGCAGTGGCCTGAGTGGCC CAGGCAGCGCCGGCAGCGGTGCGGGGGCAGCAGGCAGCCTGGAGAATGGCAAGACGGGAGCCGACGTGGT GGACCTCACACTGGACAGCTCGTCGTCCTCGGAAGATGAAGATGAGGACGAGGACGACGATGAGGACGAG GACGAGGGCCCCCGGCCGAAGCGCCGCTGCCCGTTCCAGAAAGGCCTGGTGCCCGCGTGCTGACTCCGCT GCGGGGGCGCTGGAGGGCGGGAGCCGCACCCGTCCATCATTCCCTCGCCCACCCCTCCCCGGCTCCCGGC TCCTGTACCTCTGGACTCTCCTCTTGGGGGGATTAAAAAAAGTAAAATGACAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_021501
• Insert Size: 1524 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC025159, AAH25159
• RefSeq Size: 1676 bp
• RefSeq ORF: 1524 bp
• Locus ID: 59004
• UniProt ID: Q9JM05
• Cytogenetics: 10 C1
• Gene Summary: Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53/TP53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. Mediates sumoylation of CEBPA, PARK7, HERC2, MYB, TCF4 and RNF168. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation (By similarity).[UniProtKB/Swiss-Prot Function]