Ngly1 (NM_021504) Mouse Untagged Clone

CAT#: MC202185

Ngly1 (untagged) - Mouse N-glycanase 1 (Ngly1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ngly1
• Synonyms: 1110002C09Rik; Png1; PNGase
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC028961 sequence for NM_021504
  CGCAGCCATGGCGTCGGCCACACTGGGCAGCTCGTCGAGCTCTGCGTCCCCGGCCGTGGCCGAGCTATGC CAGAACACCCCGGAGACCTTTCTGGAGGCCTCCAAGCTGCTGCTCACCTACGCCGACAACATCCTGAGAA ACCCCAGTGATGAAAAATACAGATCCATCCGTATTGGGAACACAGCGTTTTCTACTAGACTCTTGCCTGT CAGAGGAGCTGTTGAGTGTTTATTTGAAATGGGCTTTGAAGAGGGAGAAACGCATCTTATCTTTCCTAAA AAAGCTTCAGTGGAACAGCTACAGAAAATCCGTGATCTGATTGCCATAGAGAGAAGCAGCAGACTGGACG GATCCAGTAAGAAGGTGCAATTCTCTCAGCACCCGGCTGCTGCCAAGCTTCCTCTGGAGCAGTCTGAAGA TCCTGCTGGGTTAATACGGCACTCAGGAAACCAAACAGGGCAGCTGCCAAGCCTACCCTCTGCTCCAATG GTTGTTGGTGATTCAACCATCTTAAAAGTTCTTCAGTCCAACATTCAGCATGTGCAGCTCTATGAAAACC CTGTCCTCCAGGAGAAAGCATTGACTTGTATCCCAGTCAGTGAACTAAAAAGAAAAGCTCAAGAAAAGTT ATTCAGAGCTAGAAAGTTAGATAAAGGTACTAATGTAAGTGATGAGGACTTCCTTCTACTGGAGCTCCTA CACTGGTTTAAAGAGGAGTTTTTTCGCTGGGTGAATAACATTGTGTGCAGCAAATGTGGTGGAGAGACTA GATCTAGAGATGAGGCATTGCTGCCCAATGATGATGAACTGAAGTGGGGTGCAAAGAATGTGGAGAATCA TTACTGTGATGCCTGCCAGTTGAGCAACCGCTTCCCAAGATATAACAACCCCGAGAAACTTTTGGAAACA AGATGTGGACGCTGTGGTGAATGGGCCAATTGTTTTACTCTGTGCTGCCGTGCATTAGGGTTTGAAGCTC GATATGTGTGGGATTACACAGACCATGTTTGGACAGAAGTCTATTCTCCATCTCAGCAGCGGTGGCTACA CTGTGATGCATGTGAGGATGTCTGTGACAAACCCCTCCTTTATGAAATAGGATGGGGCAAGAAGCTTTCC TATATAATAGCATTCTCTAAAGATGAGGTAGTTGATGTCACCTGGCGATACTCTTGTAAACATGATGAGG TGATGTCCAGGAGGACCAAGGTTAAAGAAGAGTTACTTCGAGAAACTATTAATGGGCTTAATAAGCAGAG ACAGCTATCATTATCAGAAAGCAGAAGGAAAGAACTTCTCCAGAGGATAATTGTGGAGCTTGTTGAATTT ATATCTCCTAAAACCCCAAGACCTGGAGAACTTGGTGGAAGGGTATCCGGGTCATTGGCTTGGAGAGTGG CCCGAGGTGAGACGGGTCTAGAGAGAAAGGAGATTCTGTTCATCCCTTCTGAAAATGAAAAGATTTCTAA GCAGTTTCACCTCCGTTATGATATTGTGAGAGACCGTTACATTCGTGTCTCAGACAACAACATAAACATT TCTGGATGGGAGAATGGTGTGTGGAAAATGGAATCCATATTCAGAAAAGTCGAGAAAGACTGGAACATGG TTTATTTGGCCCGAAAAGAAGGATCATCTTTTGCTTATATTTCCTGGAAGTTTGAATGTGGGTCAGCTGG TCTAAAAGTAGATACTGTTTCCATCCGCACAAGTAGCCAAAGCTTTGAGTCTGGATCAGTGAGGTGGAAA CTGCGATCTGAGACAGCTCAAGTCAACCTGCTGGGAGATAAAAATCTACGTTCCTATAATGATTTTTCTG GTGCCACAGAGGTTACATTAGAAGCAGAGTTAAGCAGAGGAGATGGAGACGTTGCTTGGCAACATACCCA ACTGTTTAGACAAAGCTTAAATGACAGTGGAGAAAATGGCTTGGAAATAATTATAACGTTCAATGACCTC TGAAAGCCTGAACTTCAGGGAAATGCTGGCTACAATCACCGACCCGCCATGGCCTCCAGTAGTGTATTAG CTCAGTGTGTATCTAGTTGGCAGTTCACCATCCTGTGCAACAGGTTTCTTTTGCTGGCTGTACATCATGT AATCCTCATTAAAGTATATCTTCATAGTGTGGACCATAAGAAATCTTCAATTAAATGCTAACTTCTGCCT GTGATTAGAATTTGGCATAAAATATTTGGCATAAAGTAACTGTATGAAATTCTAATCTAAACACTGTTAC TAAAGTTGTAAGTGAAAATGATGGTATTCATGATTAAATGCTGTTCATGAATGTGATACAATCTAGGGCT ACTAGTTACTTAACATTTTCTGATTAGATTTTTCCAAACATTCTCAATTTTGAATAACTGTCAAAGTCAA GTATCTTATGTCATATGATGCATGAAGGTTTTTATCATTGTAAATATCTGGACAGTTAAAAAATAAAATG GATTATTTTTGCTTTAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_021504
• Insert Size: 1956 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC028961, AAH28961
• RefSeq Size: 2480 bp
• RefSeq ORF: 1956 bp
• Locus ID: 59007
• UniProt ID: Q9JI78
• Cytogenetics: 14 7.08 cM
• Gene Summary: Specifically deglycosylates the denatured form of N-linked glycoproteins in the cytoplasm and assists their proteasome-mediated degradation. Cleaves the beta-aspartyl-glucosamine (GlcNAc) of the glycan and the amide side chain of Asn, converting Asn to Asp. Prefers proteins containing high-mannose over those bearing complex type oligosaccharides. Can recognize misfolded proteins in the endoplasmic reticulum that are exported to the cytosol to be destroyed and deglycosylate them, while it has no activity toward native proteins. Deglycosylation is a prerequisite for subsequent proteasome-mediated degradation of some, but not all, misfolded glycoproteins.[UniProtKB/Swiss-Prot Function]