Nedd9 (NM_017464) Mouse Untagged Clone

CAT#: MC201926

Nedd9 (untagged) - Mouse neural precursor cell expressed, developmentally down-regulated gene 9 (Nedd9), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nedd9
• Synonyms: Cas-L; CasL; HEF1; MEF1; Nedd-9; p105
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC053713 sequence for NM_017464
  AAGAGGTAATCCGGGATCTGCAGAGAGAGAAGCATAAGGACACAGAGGGACGAGTTCCCATCCCTGCCTG CCTACCTAGAAGAGATCAGGGTGTCCAGGGTCACAAGATGGTCTGTCCACTGCGGTTCTGCGTTCAGGCT AGTGAAGTGTGAGTCTCCAGCACCAGACGCTGGCGGAGAAGCACGGCAGAGTTGTTCATGGGGAGATATG ACAAGGAGGAAACAGGCCAGCAGACTGCAGCCCTGGGACTGTCCTGGAGATCCAGAGTGCCAACGCTCCT CAAGAGAAAATGTGGGCGAGGAATCTTATGGCAAGGGCCTTGTATGACAACGTCCCTGAGTGTGCTGAGG AGCTGGCCTTCCGCAAGGGAGACATCCTAACTGTCATAGAGCAGAACACAGGAGGGCTTGAGGGATGGTG GCTGTGTTCCCTCCACGGTCGCCAAGGCATTGTCCCAGGGAACCGGGTGAAGCTTCTGATTGGTCCAGTG CAAGAGACCCCCGGTCATGAGCAGCCTACTCCTGGACCTATGCATCAGACCTTTGGCCAACAGAAACTCT ATCAAGTGCCAAATTCCCAGGCAGCATCTCGGGATACCATCTACCAAGTGCCACCCTCCTACCAGAATCA GGGAATTTACCAAGTACCCACTGGCCATGGCACTCCAGAACAAGATGTATATCAAGTACCACCATCAGTT CAGAGGAACATTGGCGGCACTAATGGACCCCTTCTAAGCAAAAAGGTGATCACCCCAGTGAGGACGGGCC ATGGCTATGTGTACGAGTACCCATCCAGATACCAAAAGGATGTCTACGATGTCCCTCCTTCCCACAGCAC TCAAGGGGTATATGACATCCCTCCTTCCTCAGTAAAAGGCCCTGTGTTTTCAGTTCCAGTGGGAGAGATA AAACCTCAAGGGGTATATGACATTCCCCCCACCCAAGGGGTCTATGCCATTCCACCATCGGCTTGCCGAG ATGAGGCAGGGCTCAGGGAAAAGGAATATGATTTCCCTCCTCCAATGAAGCAAGATGGAAAACCAGACAC CAGACCTGAGGGGGTTTATGACATCCCTCCAACCAGCACCAAGACAGCAGGCAAGGACCTTCACATCAAA TTCCCCTGTGATGCTCCAGGAGGTGTCGAACCAATGGCACGAAGACACCAGAGCTTTTCCCTGCACCATG CACCCTCTCAGCTGGGACAGTCTGGGGACACTCAGAGTGATGCCTATGATGTCCCCCGGGGAGTTCAGTT TCTGGAGGTACCAACAGAAACCAGTGAAAAGGCAAATCCGGAGGAAAGAGACGGTGTCTACGATGTCCCT CTGCACAACCCAGCAGATGCCAAAGGCTCTCGGGACGTGGTAGATGGGATCAACAGACTGTCTTTCTCCA GCACTGGCAGTACCAGGAGTAACATGTCCACCTCTTCCACCTCCTCAAAGGAGTCTTCACTGTCAGCCTC CCCGTCTCAAGACAAAAGGCTCCGACTGGACCCAGACACAGCCATAGAGAAGCTCTATCGGCTCCAGCAG ACCCTGGAGATGGGTGTGTGCAGCCTCATGTCACTGGTCACCACAGACTGGAGGTGCTACGGATACATGG AAAGGCACATCAATGAGATCCGCACCGCGGTGGACAAAGTAGAGCTGTTCTTACGAGAATACCTCCATTT TGCCAAGGGAGCTTTAGCCAATGCCTCCTGCCTCCCAGAACTGGTCCTCCACAACAAAATGAAGCGGGAA CTCCAAAGAGTAGAAGATTCCCACCAGATTCTAAGCCAAACCAGCCATGACTTGAATGAATGCAGCTGGT CCCTGAATATTTTAGCTATCAATAAGCCCCAAAATAAGTGTGATGACCTAGACCGGTTTGTGATGGTCGC CAAGACAGTGCCAGACGACGCCAAACAACTGACCACCACCATCAGCACCTACGCGGAGACCCTCTTTAGA GCAGATCCTGCCAATTCCCATCTGAAGAATGGGCCCAACAGCATCATGAACTCAAGCGAGTACACACATC CGGGCTCCCAGATGCAGCCACTGCATCCTGGTGACTACAAAGCCCAGGTCCACAGTAAGCCGTTGCCTCC TAGTCTAAGCAAGGACCAGCCACCAGACTGCGGTAGCAGTGACGGTTCTGAGCGGAGTTGGATGGATGAT TATGATTATGTTCACCTACAGGGCAAGGAGGAGTTTGAGCGACAGCAGAAGGAGCTCTTGGAAAAGGAGA ACATCATGAAGCAGAGTAAGGCGCAGCTGGAGCATCACCAGCTGAGTCAGTTCCAGCTGTTGGAACAAGA GATCACCAAGCCTGTGGAGAATGACATCTCTAAATGGAAGCCCTCTCAGAGCCTCCCAACCACCAACAAC AGTGTGGGTGCTCAGGATAGGCAGTTGCTTTGCTTCTACTATGACCAGTGCGAGACCCATTTCATTTCCC TACTCAACGCCATCGACGCCCTCTTCAGCTGCGTCAGCTCAGCCCAACCCCCACGGATCTTTGTGGCGCA CAGCAAGTTTGTCATTCTTAGTGCGCACAAACTGGTGTTCATTGGAGACACTCTGACAAGGCAGGTGGCT GCCCAGGACATTCGCAACAAAGTCAGGAACTCCAGCAACCAGCTCTGCGAACAGCTCAAGACGATAGTGA TGGCGACCAAAATGGCCGCCCTCCACTACCCCAGTACCACCGCCTTGCAGGAAATGGTGCACCAGGTGAC AGACCTGTCCAGAAATGCTCAGCTGTTTAAGCGTTCCTTGCTGGAGATGGCCACCTTTTGAGAAGACAAA GAAGTGGAAGGAACTGGGTGAATAATTACTAAGGAAAACTGGAAATACTATCTAGTTTTTGTAAATGCTA TCTATTTTTGTAGATATTTTATATGAAATTGAAATATTTCGATGTTTTTGTGAGTTAGTCGATTTTCATC AATTCAGGGAGCTGAAGCTTGGATTTATTTTGTTTCCCCTGTGTGGTTCTGATATAAACATATAAGTATC TAAGACATAAGTTGTACAGAACTGTGTCCACGTTTGTGTATGCCTACATATCCATATTTGTTTATCTGTG TGTCTGATACAGCCCATTAAAAACATGAATTGAGAAGCAAAAAAAAAAAAAAAAAAAAAAAAAAG
• Restriction Sites: RsrII-NotI
• ACCN: NM_017464
• Insert Size: 2502 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC053713, AAH53713
• RefSeq Size: 3145 bp
• RefSeq ORF: 2502 bp
• Locus ID: 18003
• UniProt ID: O35177
• Cytogenetics: 13 A3.3- A4
• Gene Summary: Docking protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. May function in transmitting growth control signals between focal adhesions at the cell periphery and the mitotic spindle in response to adhesion or growth factor signals initiating cell proliferation. May play an important role in integrin beta-1 or B cell antigen receptor (BCR) mediated signaling in B- and T-cells. Integrin beta-1 stimulation leads to recruitment of various proteins including CRK, NCK and SHPTP2 to the tyrosine phosphorylated form (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) contains an alternate 5'-most exon and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is the same length as isoform 1.