Wee2 (NM_201370) Mouse Untagged Clone

CAT#: MC201865

Wee2 (untagged) - Mouse WEE1 homolog 2 (S. pombe) (Wee2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Wee2
• Synonyms: Gm1065; Wee1B
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC052883 sequence for NM_201370
  AAGAGGTAATCCGAAGTGGCAAGTGATGTGACGCGAGGAAGGGAGAACTGACCTACTCGGAGGCGGTGGG GAAAGGATCAGATCACCTGGTTCACTGGCTTGTAACCAAGAACCTGCTCCTGGGCTCAGAATTTCAGCTG ATGGTGACTTCTCTGTTGGTAACCTGTGCCTTCCTTTCCCTTGGATGGTTTTTTATGGCCACCCGTGGAA CGCTGAGCCTCTGACAAAGATTTATAGTGTTTTCCACTCCGAGACCTCCTTGTCTGCACTGAGATGGCCG ACACAGAGACTGACCAGGGACTGAACAAGAAATTAAGCTTCTCCTTTTGTGAAGAAGACACTGAGAGTGA AGGGCAGATGACTGCACAAGACATCGGAGGGGCCCAGAGCCAAAAGCCTGGGAGGGAAGAAAGTGAGGAG TTGGAGGGTCCCGTGCCTCCTACCAGAGATGAACTTCATACTTCTCTGAGCAGAGACAAGGGAAGTCCAG GTCCTGATCTTTGGAAATCAGTTTCAGGCCCTCCCAAATGCCCAGAGACACCAGTATTACACAGCAAGAG CAAGCCGCCAGTCCCTACCAACTTCTCCACTCCCAAAAATTCGCTGGGTCAGCCAGAGATTTCTCTGCTA GAGAAGCTCTCCTCCCGAAGCTCTAGACATATGAGGCTCACACCTGCTTCCCTTGTGGATGAGAAGACCT CTTTGTCTCTGGTCAATATCAACCCATTCACTCCGGAGACCTACAGAAAATTACTCCTTCAATCAAAGGG CAAGAGGAAGACCAGAGACTATCTTGAGGAAACTGGAGAAGAGGAAAGCAAATCAGTACAGTGGCTGCCT GCTAAGAGAAGTATTCTACAAGAAACTAATATGGCTTCCCGCTATGAAAAAGAATTCTTCGAGATAGAAA AAATCGGGGTTGGAGAATTTGGGACAGTCTACAAGTGCATTAAGAGGCTGGATGGATGCATTTATGCAAT AAAGCGCTCTGCAAAGTCCTTTTCAGGATTATCAAATGAATTGGATTTGCATGAAGTTTATGCTCATGCA GTGCTTGGCCATCATCCCCATGTGGTACGCTACTACTCATCATGGATAGAAGATGATCATGTGGTCATTC AAAATGAATACTGTAATGGTGGGAGCCTGCAAGCTGCTATATCTGAAAACACTGCGTCCAATAATCACTT CCAAGAGCCCAAACTCAAAGACATCCTTCTGCAGATTTCCTTGGGACTTAAGTACATCCACAATTCTGGC ATGGTACACCTGGACATCAAACCCAGTAACATCTTCATTTGTCATAAGATGCAGTGTGACTCTCCTGTAG GCCCAGAAGAGGCTGAAAGTGAAGCTGACTGGTTTCTCAATGCCAGTGTGATGTATAAAATTGGTGACCT GGGCCACGCGACATCAATCAGCAAACCTAAAGTGGAAGAAGGAGATACTCGTTTCCTGGCTAATGAGATC TTGCAAGAGAATTACCAACACCTCCCCAAAGCAGACATATTTGCCTTAGGATTAACAATCGCAGTGGCTG CAGGAGCAGAGTCTTTACCCATCAATGGTGACATGTGGCATCATATCCGCAAAGGGAACTTTCCAGAGAT TTCCCAAGAGCTCTCGGATGACTTTTATGGTCTACTCAAGAACATGATCCACCCAGCTCCAAAGGAGAGA CCATCTGCAGCAGCTCTGGCCAGAAGTCGAATTCTCTGGCCTTTCTTGGAGAAGACTGATGAACTCCAGA AACAACTGAATTTGGAGAAATCCAAGACAGCCACACTGAAAAGGGAACTGAAAAAAGCTCGGCATATCCA GACCCCTCAGAGAGAAGTCCACCATTGTTATTATCAGATCTGCAAAGGCTCAAGACACCTCCTGGTGGGA GGAAGGAGGAAGGCGCCCTCAAGCTTCACCCGTGGGACATCCTCCGTGTAGGAACTCACTTCTGGCCTTT TGCTACACAACACAGCCTTTTGAAAACTCTTACAGGATACAAAACTGAACCAGCTTGAATGTACAAAGAT CTCAAAAGCCATCCTGATGTGGCATCTATAGGTAAGATCACCGAGCTTCCAGGGTTTCTCCTTAGCAACG GTCTTGTGTATCGTAAGTACTGGAGTCTTCTTTTCCCTAGTGATGTATTTCTAGAGCTGCTTACTAAGGA CTCCTAATGAATAATCAGAAAACATGGCTCTGAACTGAAAGGAGAAGCAGAAATGTGCTACTTTGGCCTA TGGATTAAGAAGCTGCCGTTACTTATTCTTCACTGGGGTCCAAAGAGCTTTCCCTGTACATAAAAAGGGA TAAAATATGGCTTTAAGTGCCAGCTTCCAAGAGAATGTTGCCTGTATTTCAGAGTGCTTTCCAAGATCTG TCTGCTCCTCCAATCACCAGCTCCTTTGAGCAAGGAATTTGCCTACTATACTTTCTGAATGGAAGCAATC TCTTTTCTGATTTTGCATTATTAAATAAAAGACTATCATTGTGTGAAACAACTAGAGTAAGACTATAATT TGGACATTCCTGAGCTGCTTATTAATTTGTGAAACACAATCAGCAGAGGCAGAGGACCCAGGAATTTTGG TGTACCTGTTCCCCGTGTGTTCCTCACGTCTTAGGTCTCTGTGGCTGACTCCAGTTGCTTTTCAGTTTTG AACATCATAGCATCATCTGGTTCTCTTGATTTTTTTTTTTTACACCAGAACTCATCCTTTTGTTTTCTTG CTTTTTAACATATTTTAATCCAGATCATTGTAAATAAAGATTTTATCTTATCCAAAAAAAAAAAAAAG
• Restriction Sites: RsrII-NotI
• ACCN: NM_201370
• Insert Size: 1668 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC052883, AAH52883
• RefSeq Size: 2798 bp
• RefSeq ORF: 1668 bp
• Locus ID: 381759
• UniProt ID: Q66JT0
• Cytogenetics: 6 B1
• Gene Summary: Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits CDK1 and acts as a key regulator of meiosis during both prophase I and metaphase II. Required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, by phosphorylating CDK1 at 'Tyr-15', leading to inhibit CDK1 activity and prevent meiotic reentry. Also required for metaphase II exit during egg activation by phosphorylating CDK1 at 'Tyr-15', to ensure exit from meiosis in oocytes and promote pronuclear formation.[UniProtKB/Swiss-Prot Function]