Tut1 (NM_197993) Mouse Untagged Clone

CAT#: MC201748

Tut1 (untagged) - Mouse terminal uridylyl transferase 1, U6 snRNA-specific (Tut1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tut1
• Synonyms: 2700038E08Rik; PAPD2; Rbm21; star-PAP; TUTase6; U6-TUTase
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC025499 sequence for NM_197993
  CCACGCGTCCGCGGACGCGTGGGCTTCCCCGCACGCACGTTACAATTTTTAGTTGTGTGTTCCGGTGTGT TTCCGGCGAAATAGGCTGTAGGCGTTCCGTACCGGAGAGTGGCTATGGCGGCGGTGGATTCGGACGTTGT ATCGCTGCCGCGTGGCCGCTTTCGCTGCTGCCTCTGCGATGTTACTACAGCCAACCGACCCAGCCTCGAT GCCCACTTGAAAGGCCGGAAGCACCGGGATTTGGTGCAACTCCGAGCTACCAGGAAGGCACAGGGACTCC GAAGTGTGTTTGTCAGTGGCTTCCCCAGGGATGTGGGTTCTGCTCAGCTCTCTGAGTACTTCCAGACATT TGGCCCTGTGGCCAATATTGTCATGGACAAGGACAAGGGGGTGTTTGCCATCGTGGAGATGGGAGACATA AGTGCTCGGGAGGCTGTCTTATCACAGCCCAAGCACAGCCTTGGGGGACATGGACTTCGAGTCCGGCCAA GGGAGCAGAAGGAGTTCCAGAGCCCAGCTTCCAAGTCTCCCAAAGGAGTGGACTCAAGTAGTCACCAGCT GGTCCAAGCACTGGCTGAGGCTGCGGATGTGGGGGCCCAGATGGTGAAGCTTGTGGAACTGAGGGAGTTG TCTGAGGCTGAGCGGCAGCTTCGGAACCTTGTTGTGGCCCTGATGCAGGAGGTCTTCACAGAGTTCTTCC CTGGCTGTGTGGTCCATCCTTTTGGCTCTACTGTAAATAGCTTTGATGTTCATGGCTGTGATCTCGACCT CTTCTTGGACATGGGTGATATGGAAGAGACCGAGCCAGACCCAAAGGCTCCAAAGGTTCCAGAGACTTCA TCCTTGGACTCAGCCCTTGCTTCTTCCCTGGATCCTCAGGCGCTGGCCTGCACCCCAGCTTCTCCTCTAG ACTCACTGTCTCCAACTTCTGTTCAAGAGTCTGAGTCCCTGGACTTTGACACCCCATCTTCTCTGGCACC ACAGACACCCGACTCTGCTTTGGGCTCTGACACTGTCACCTCTCCTCAGTCTCTGCCTCCAGTTTCACCA CTGCAGGAGGACAGGAAAGAGGGGAAACAGGGGAAAGAGCTAGAATTAGCAGAAGAAGCCTCAAAGGACG AGAAGGAGGAGGCAGCAGCAGTGTTAGAGCTGGTGGGATCTATTCTCCGTGGCTGTGTCCCTGGAGTGTA CCGAGTCCAAACTGTGCCCTCTGCCCGGCGTCCTGTGGTCAAGTTCTGTCATCGGCCTTCAGGTCTTCAT GGAGATGTATCCCTCAGTAACCGGTTGGCCTTATATAATTCACGTTTCCTGAATCTCTGCTCTGAGATGG ATGGTCGAGTCCGGCCCCTTGTGTATACCTTACGCTGCTGGGCTCAGCATAATGGGCTGTCAGGAGGTGG CCCCCTTCTCAATAACTACGCCTTGACGTTGTTAGTGATCTACTTCCTTCAGACCAGAGACCCTCCAGTA CTTCCTACTGTGGCCCAGCTTACCCAGAGAGCAGGTGAAGGGGAACAGGTAGAAGTTGATGGCTGGGACT GTAGCTTCCCTAAGGATGCCTCAAGATTGGAGCCCAGCACCAACGTGGAGCCTCTCAGTTCCCTGCTGGC CCAGTTCTTCTCCTGCGTATCTTGCTTGGATCTTTCTGGCTCCCTGCTGTCCCTGCGGGAAGGTCGGCCA TTGATGGTGGCAGAGGGCCTGCCTTCTGATCTCTGGGAAGGGCTGCGCCTTGGCCCCATGAATCTCCAGG ACCCCTTTGACCTGAGTCACAATGTTGCAGCCAATGTGACCGGCCGGGTGGCTAAACGTCTGCAGAGCTG TTGTGGGGCAGCAGCCAGTTACTGCCGAAGTCTCCAGTACCAGCAGCGTTCCTCCCGGGGCCGGGACTGG GGACTGCTCCCGCTTTTGCAGCCCAGCTCCCCTAGCTCCCTGCTGTCTGCCAAGCTCATCCCCTTACCCT CTGCCCCCTTTCCACAGGTCATTATGGCTTTGGTGGATGTGTTAAGGGAAGCACTAGGATGCCACATAGA ACAGGGAACCAAGAGACGACGGTCAGAAGGTGCCAGAATTAAAGACTCTCCCTTGGGAGGGGTGAACAAA AGACAGAGACTTGGTGGGCAAGAAAAGAGCTTCGAGGAAGGGAAAGAGGAGCCACAGGGATGTGCAGGGG ACCACAGTGAAAATGAGGTAGAAGAAATGGTAATAGAGGTTCGAGAGACACCTCAGGACTGGGCCTTGTT GCACTCGGGACCACCAGAGGAGGAGTTGCCCCTGATGACTGCAAACTGTCTAGACAAGGCAGCTGAGCAT AATCCCATGAAACCTGAAGTGGCTGGAGAAGGGTCTCAGGGTGAGACAGGGAAGGAGGCATCACACCCAT CATCAGTGAGCTGGCGCTGTGCCTTGTGGCACCAAGTATGGCAGGGGAGGCGGCGTGCCCGGAGACGATT ACAGCAACAAACCAAGGAAGAAGGTAGAGGAGGTCCCACCACAGGAGCAGAGTGGCTGGCAATGGAAGCT CGTGTGACCCAGGAACTAAAAGGACCCAATAGTGAGCAAGAGAGGCCGCCGGGGGAGCCCCTCCTCAGCT TTGTGGCATCTGCCTCCCAGGCTGAGCAGACACTCACTGTGGCCCCACTCCAGGACTCCCAAGGCCTGTT CCCTGGTCTTCATCATTTTCTACAGGGTTTTATCCCTCAAGCACTTAAAAATCTCCTCAAGTGAGGAACC AAACCTCAAAAACAATAAAGCTGCTAGTTTATTTCATACAGACAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_197993
• Insert Size: 2610 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC025499, AAH25499
• RefSeq Size: 2788 bp
• RefSeq ORF: 2610 bp
• Locus ID: 70044
• UniProt ID: Q8R3F9
• Cytogenetics: 19 A
• Gene Summary: Poly(A) polymerase that creates the 3'-poly(A) tail of specific pre-mRNAs. Localizes to nuclear speckles together with PIP5K1A and mediates polyadenylation of a select set of mRNAs, such as HMOX1. In addition to polyadenylation, it is also required for the 3'-end cleavage of pre-mRNAs: binds to the 3' UTR of targeted pre-mRNAs and promotes the recruitment and assembly of the CPSF complex on the 3' UTR of pre-mRNAs. In addition to adenylyltransferase activity, also has uridylyltransferase activity. However, the ATP ratio is higher than UTP in cells, suggesting that it functions primarily as a poly(A) polymerase. Acts as a specific terminal uridylyltransferase for U6 snRNA in vitro: responsible for a controlled elongation reaction that results in the restoration of the four 3'-terminal UMP-residues found in newly transcribed U6 snRNA. Not involved in replication-dependent histone mRNA degradation (By similarity).[UniProtKB/Swiss-Prot Function]