Slc27a1 (NM_011977) Mouse Untagged Clone

CAT#: MC201650

Slc27a1 (untagged) - Mouse solute carrier family 27 (fatty acid transporter), member 1 (Slc27a1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Slc27a1
• Synonyms: Fatp; FATP1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC028937 sequence for NM_011977
  ATCCCCTGAAGGGACCCACCAGCTAGGCCTGAGCTCAGAACTTCCCAGTCCAGACTTCTGTGAGAACCTG CGAGGGAGCAGCAAGGGCCAGGGATCTCTCTCTCCACCCCCGGGTGTGAATTTCGACTTTCTCGGAGTCT GGAATGCTGAGAAGCCTCGGGGCTTGGATCTAGGACAAGCTGGATCAGGCAAGCTCCAGCACAGGATGCG GGCTCCTGGAGCAGGAACAGCCTCTGTGGCCTCACTGGCGCTGCTTTGGTTTCTGGGACTTCCGTGGACC TGGAGCGCGGCGGCGGCGTTCTGTGTGTACGTGGGTGGCGGCGGCTGGCGCTTTCTGCGTATCGTCTGCA AGACGGCGAGGCGAGACCTCTTTGGCCTCTCTGTTCTGATTCGTGTTCGGCTAGAGCTGCGACGACACCG GCGAGCAGGAGACACGATCCCGTGCATCTTCCAGGCTGTGGCCCGGCGACAACCAGAGCGCCTGGCACTG GTGGACGCCAGTAGTGGTATATGCTGGACCTTCGCACAGCTGGACACCTACTCCAATGCTGTAGCCAACC TGTTCCGCCAGCTGGGCTTTGCACCAGGCGATGTGGTGGCTGTGTTCCTGGAGGGCCGGCCGGAGTTCGT GGGACTGTGGCTGGGCCTGGCCAAGGCCGGTGTGGTGGCTGCTCTTCTCAATGTCAACCTGAGGCGGGAG CCCCTGGCCTTCTGCCTGGGCACATCAGCTGCCAAGGCCCTCATTTATGGCGGGGAGATGGCAGCGGCGG TGGCGGAGGTGAGCGAGCAGCTGGGGAAGAGCCTCCTCAAGTTCTGCTCTGGAGATCTGGGGCCTGAGAG CATCCTGCCTGACACGCAGCTCCTGGACCCCATGCTTGCTGAGGCGCCCACCACACCCCTGGCACAAGCC CCAGGCAAGGGCATGGATGATCGGCTGTTTTACATCTATACTTCTGGGACCACCGGGCTTCCTAAGGCTG CCATTGTGGTGCACAGCAGGTACTACCGCATTGCTGCCTTTGGCCACCATTCCTACAGCATGCGTGCCGC CGATGTGCTCTATGACTGCCTGCCACTCTACCACTCTGCAGGGAACATCATGGGTGTGGGGCAGTGCGTC ATCTACGGGTTGACGGTGGTACTGCGCAAGAAGTTCTCCGCCAGCCGCTTCTGGGATGACTGTGTCAAGT ACAATTGCACGGTAGTGCAGTACATAGGTGAAATCTGCCGCTACCTGCTGAGGCAGCCGGTTCGCGACGT GGAGCAGCGACACCGCGTGCGCCTGGCCGTGGGTAATGGGCTGCGGCCAGCCATCTGGGAGGAGTTCACG CAGCGCTTCGGTGTGCCACAGATCGGCGAGTTCTACGGCGCTACCGAGTGCAACTGCAGCATTGCCAACA TGGACGGCAAGGTCGGCTCCTGCGGCTTCAACAGCCGTATCCTCACGCATGTGTACCCCATCCGTCTGGT CAAGGTCAATGAGGACACGATGGAGCCACTGCGGGACTCCGAGGGCCTCTGCATCCCGTGCCAGCCCGGG GAACCCGGCCTTCTCGTGGGCCAGATCAACCAGCAGGACCCTCTGCGGCGTTTCGATGGTTATGTTAGTG ACAGTGCCACCAACAAGAAGATTGCCCACAGCGTTTTCCGAAAGGGCGATAGCGCCTACCTCTCAGGTGA CGTGCTAGTGATGGACGAGCTGGGCTACATGTATTTCCGTGACCGCAGCGGGGACACCTTCCGCTGGCGC GGGGAGAACGTGTCCACCACGGAGGTGGAAGCCGTGCTGAGCCGCCTACTGGGCCAGACGGACGTGGCTG TGTATGGGGTGGCTGTGCCAGGAGTGGAGGGGAAAGCTGGCATGGCAGCCATCGCAGATCCCCACAGCCA GTTGGACCCTAACTCAATGTACCAGGAATTACAGAAGGTTCTTGCATCCTATGCTCGGCCCATCTTCCTG CGTCTTCTGCCCCAGGTGGATACCACAGGCACCTTCAAGATCCAGAAGACCCGGCTGCAGCGTGAAGGCT TTGACCCCCGTCAGACCTCAGACAGGCTCTTCTTTCTAGACCTGAAGCAGGGACGCTATGTACCCCTGGA TGAGAGAGTCCATGCCCGCATTTGTGCAGGCGACTTCTCACTCTGAGCCTGGAGAGTGGGCTGGGCCTGG ACTCCTGAGACCTGGGAGCCTGACACCCCTCTTCGGGTGCTTCTCCTGCCTGGCCACATGGACAGCAGCA CCTGTGAGAGTAGGAAAATGGAACCTGAGTGGCTGGGACCCCTCTCCTACTTCCCACTATGCATCCATTT TGCCTCTGCCTTGATCTTTTTCTCCATCTCTTTTCTCCCTACCCAGCAGGAGCCCCACAAACACATGTTG GCTGCTGTGTCCTGCAGTTGGACCAGTGTCCAGGGGTACAGGCTTCAGGCTGTGACCCACACTGGTACCC ACCTCCCTTTCCTATTGTGCCTTAGGTTCATCCACGGTTCCCCTGTGGAGCAAGTGGGGGCCCACATAGC TGCTGTCCCTGCTGAGGGTTGGTAGCAATCACACCCTCATGTCAGCTGGGAGACACGCGCAGTCTCCCAC TGACCCCCAATCAACTGAAAATATTGTTTTGACTACTTTTTGTTTTTTTGTTTTTTTGTTTTTTTTTTTG GTTTTTCGAGACAGAGTTTCTCTGTATAGCCCTGGCTGTCCTGGAACTCACTTTGTAGACCAGGCTGGCC TCGAAATCAGAAATCCACCTGCCTCTGCCTCCCTAGTGCTGGGATTAAAGACATGCGCCCCCCCCAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_011977
• Insert Size: 1941 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC028937, AAH28937
• RefSeq Size: 2828 bp
• RefSeq ORF: 1941 bp
• Locus ID: 26457
• UniProt ID: Q60714
• Cytogenetics: 8 B3.3
• Gene Summary: Mediates the ATP-dependent import of long-chain fatty acids (LCFA) into the cell by mediating their translocation at the plasma membrane (PubMed:7954810, PubMed:9786857, PubMed:9671728, PubMed:10471110, PubMed:12235169, PubMed:11970897, PubMed:15699031, PubMed:28178239). Has also an acyl-CoA ligase activity for long-chain and very-long-chain fatty acids (PubMed:10593920, PubMed:12235169, PubMed:12937175). May act directly as a bona fide transporter, or alternatively, in a cytoplasmic or membrane-associated multimeric protein complex to trap and draw fatty acids towards accumulation (PubMed:14991074, PubMed:15897321). Plays a pivotal role in regulating available LCFA substrates from exogenous sources in tissues undergoing high levels of beta-oxidation or triglyceride synthesis (PubMed:12235169). May be involved in regulation of cholesterol metabolism (PubMed:12235169).[UniProtKB/Swiss-Prot Function]