Vrk3 (NM_133945) Mouse Untagged Clone

CAT#: MC201539

Vrk3 (untagged) - Mouse vaccinia related kinase 3 (Vrk3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Vrk3
• Synonyms: AI428238
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC024839 sequence for NM_133945
  GTTTCTGCGGGGCAACTGTAAAGGCTGGTGTCGAGATGGAGTGTCTAACCCAGTAATCCAAGGACCAGAC CCCGAGGCCACAAAGCATGATCTCCTTCTGTCCAGTCTGTGGCAAAAGTGTCAAGGTATCATTCAAGTTC TGCCCTTACTGTGGCAAGGCCCTGCCTGTAGAGGAGGATGGAGGCACCCAGTCTGCCGTGACGCCTCATG TGTCATCCGTTCCAGGCTCCAGGAGAGACCTGAACTCCAGTTTTGAAACCTCTCCCAAGAAAGTGAAATG CTCACACACTGTCACTTCTCTCCCACTCTCCCGCCACTCTGACTGTGACAGTTCTGGGTCAGACAACACC TTGACTTCTCCTGACAGGGCCACAGGCACCCGGAGCAGACCCCTGACCCCCAAAGGCAGCCCACTTTCCA ACAGGCAGAGCCCTCAGACTCTGAAGCGGACCCGGGTGACCACCTCACTCCAGGCTCTAGCCACAGGAAC AGAGCTGACAGACCAGAATGGGAAACACTGGACCCTGGGGGCCCTGCAGATCCGGGATGACCAAGGCATT CTCTATGAAGCTGAGCCTACTTCTGCCGTCCCCTCTGAATCAAGGACCCAGAAGTGGAGATTCTCACTCA AACTGGACTCCAAGGATGGGCGCCTTTTCAATGAGCAGAACTTCTTTCAGAGAGTAGCCAAGCCTCTGCA AGTGAACAAATGGAAGAAGCAGTTCTTGCTCCCGCTCCTGGCCATCCCCACCTGTATCGGCTTTGGCATT CACCAGGACAAGTACAGGTTCCTAGTATTCCCCAGCCTGGGGAGGAGCCTTCAGTCAGCCCTGGATGACA ACCCAAAGCATGTGGTATCAGAGAGATGTGTGCTTCAGGTGGCCTGCAGGCTGCTGGATGCTCTGGAGTA TCTCCATGAAAATGAGTATGTTCACGGGAACCTGACAGCTGAGAATGTCTTTGTGAATCCAGAGGATCTG AGCCAGGTGACCCTGGTGGGCTATGGCTTCACCTACCGATACTGCCCAGGTGGCAAACACGTGGCCTACA AAGAAGGCAGCAGGAGTCCACACGATGGGGACTTGGAGTTCATTAGCATGGACCTGCACAAGGGATGCGG ACCCTCCCGCCGCAGCGATCTCCAGACCTTGGGCTACTGTATGCTCAAGTGGCTTTATGGGTCCCTGCCA TGGACAAATTGCCTTCCCAACACCGAAAAGATAACTAGGCAGAAGCAGAAGTATCTGGACAGCCCCGAGC GCCTCGTGGGACTGTGTGGCCGCTGGAACAAGGCCTCAGAGACCCTGCGGGAGTACCTGAAGGTGGTGAT GGCCCTCAATTATGAGGAGAAGCCACCCTATGCCACGCTGAGGAACAGCCTAGAAGCTCTGCTGCAGGAT ATGCGGGTGTCACCCTATGACCCTCTGGACCTCCAGATGGTGCCTTAGATGGAATCCAGAGCTTCCGACT TGCAGCTTGAAGTAGAACATGAAGTAGTGTGACTGGAGGCCTGTTTGAACTCATAGCTCCTAAAAGAATC CCTTGAATGTGCATTCTCACCGCTCCCTTAGGACATATGAATCAGCACTTGTGTTGGGGAACCTGAGTCA TGTCATGTAATGTGAAACTCCTCCCTGTCTCAGCTCTGGCAGCTGTGGATGGAGGTAAGTGGATGCTGGC GGCGGCGGCGGCAGCAGCCACTCCACTCCCTATGGCATTTCTGTGATGGCATAATAAACTGTTTTTAATC AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_133945
• Insert Size: 1362 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC024839, AAH24839
• RefSeq Size: 1790 bp
• RefSeq ORF: 1362 bp
• Locus ID: 101568
• UniProt ID: Q8K3G5
• Cytogenetics: 7 B3
• Gene Summary: Inactive kinase that suppresses ERK activity by promoting phosphatase activity of DUSP3 which specifically dephosphorylates and inactivates ERK in the nucleus.[UniProtKB/Swiss-Prot Function]