Rala (NM_019491) Mouse Untagged Clone

CAT#: MC201478

Rala (untagged) - Mouse v-ral simian leukemia viral oncogene homolog A (ras related) (Rala), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rala
• Synonyms: 3010001O15Rik; AW322615; Ral; Rasl1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC031741 sequence for NM_019491
  GCTGCTGCAGAGCGGCCGGGAGGAGGGTGGGTCTCCCTAGAGCGGAGCGCCGGAGTCCTCCTCCTCCGCC GCCGCCCAGGCCGCCGCCGCTGCCGCCGCCCTCCGGACTCCTCTTGCCGCGCCCCGCGCGGAACTCCGAG CGCGGTCCGATCGGCGCAGCCGAGGCCCAAGAGCCCGCGGGTCGAGCGGCTCGCGGTGCAGATTTTTCTT AATCCTCTGAGGACTCTGGAAAACAGGATGGCTGCAAACAAGCCCAAGGGTCAGAATTCTTTGGCCTTAC ACAAAGTCATCATGGTGGGCAGTGGTGGTGTGGGCAAGTCTGCCCTGACTCTGCAGTTCATGTACGACGA GTTTGTAGAGGACTATGAACCTACCAAAGCGGACAGCTACAGGAAGAAGGTAGTCCTGGACGGGGAAGAA GTGCAGATCGACATCTTAGATACAGCGGGGCAGGAGGACTATGCTGCAATTAGAGACAACTACTTCCGAA GTGGGGAGGGATTCCTCTGTGTCTTCTCTATCACAGAGATGGAGTCCTTCGCAGCTACCGCGGACTTCAG GGAGCAGATTTTAAGAGTAAAAGAAGATGAGAATGTTCCATTTCTCCTGGTTGGTAACAAGTCAGATCTA GAAGATAAAAGGCAGGTTTCTGTAGAAGAGGCAAAAAACAGAGCTGACCAGTGGAACGTTAACTATGTGG AGACGTCTGCTAAAACGCGCGCCAACGTTGACAAGGTATTTTTTGATTTAATGAGGGAAATACGAGCCAG AAAGATGGAAGACAGCAAAGAAAAAAATGGAAAAAAGAAGAGGAAAAGTTTAGCCAAGAGAATCAGAGAA AGATGCTGCATTTTATAATCAAAGCCCAAACTCCTTTCTTATCCTGACCTGACCATACTAATAAATATAA TTTATAAGCATTGCCATTGGAGGCTCAATTGACTGAAATTACTTTAACATTTTGGAAATTGTTATGTATC ACTAAAAGCATGAATTGGAACTGCACTGGAAGTCAAATTCACTTAAAAAAGAAATTGTGTGGCTTCACCA AGAAGCAGAGTTCAACTTATTTCATAATTGCCTACATTTATCACAGTCCTGAATGTAGCGTGTAAGCTTG GTTTTTTTTTGGGGGGGGGGCAGTCTTTCTCAAACTGTTAATCAAGAGGTAAGATGGGGGCGGGGAAGAA ATGACCATTTCCTCTGGTGTTTATTATAAAGCTTAAATTTTATATTATTTTAAAATGTCTTGGTCTTCTA CTGCCTTGAAAAATGACAATTGTGAACACGATAGTTAACTGTACTACTTTTTAAGCTGTTGTTATGCAAA TCTTGAAGAAGTTACTGGCATGGTTGTTGCATATAGTAAAACTGAGAGTAATTCACCTGTGAATCTGCAT ATTAATTACCTGATGATTAACTTAGAAAAGAGATGGGAACTTATTATACATGGACATTTTTGAATATGCC TTAATTTAGAAACTGAAAAATAATCCAGTGACATCATTCTGGGTGTGTTCTCACTAGCTCGCGGGGCCAC TTGCTGCCCATGTGACGAATAACAGAAGATCTTATCTTGCCAGGCCGTGGAGAGCTACAAGAAAGATTCC TGAGAAATGTCCAACTGCTAGGAGTCTGACCAAATTACAGATGTGTGCCATACTCTGGTTCTTAGAGTTC CAGAGCTCCTTCATTGGTTTTAATAAACTCTAAGGTAGTTAAAGATGAAGGGCCAGCATATATTTATATG ATAAGTTTAGATTATGAGCGTGAGTACCAATTAAGTTTGAATGAATGCCCCTTTTATGTAGAGTGCTGGT CCCCAGTCATTGCTGTCTGCAAGTGATGCGCATTTAAGTGTTTAATCTCCTGGGAGCAGGGCACAGGGAA GACTATGAGGAGAGCTAATGTGGTCTGTACAAGGACACTGGAGGAAGTATGTTTGCTACCTGTAAGTTCT AAATGTGTACTCCTGAGGTTACAGTTTGGGTGTTAGGAGAAGTCTTTGTAGTCTCTTTATGTTCATAATG AATACTGTATCAATATTTCCTAACATGAAGCAGTCAAACATGTATGGCACATACTGACCAGACGTCAGTC TGTGATGTTTCCACTGCAAGAATTCAAGCATGCTGTTAGGAATGTAATTTCAATGGGAAGTAAACCACTA TTAGGTTTTTTTTCTTTTCCTCTGTGGGGCTAAGACACTTGATTAAACAGTTTTTCTTGTTGCACAGTCC TAATTTCATAAGGTCAAGGAGACACTCAGGCATTCCAGGAAACAAATGTGTACATGATGTTACCAGAAAC AGGCATTCAGGAACTCTACTGTCTAGATACTACATTCAGCTTACCATAAATATTTGTCCTTGAAGGGTTT GATTTAATATTTTCACAGACTAGGCCATGTGCAAAATTAGAGTTCCCTGTGTAACCCCAGCCAGCAGGCC CAGCATGGCTGCGTGCACTCATTGCAGTCTTGTTGAACCAGGGTCCTAACCACTAACATTGCAATTTGCT TTGAGACCTTCCCTGTCCTGGGTACTAGGTGCTATGAAGCCAGCTGACAGAAGCATCACATGTTTTGGGC TGATGCCGCTACCCGGTTCATTTATTTGCAATTTGAGCCATTTAAAGACCAATAAAATTCCTTTTTTTTA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_019491
• Insert Size: 621 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC031741, AAH31741
• RefSeq Size: 2697 bp
• RefSeq ORF: 621 bp
• Locus ID: 56044
• UniProt ID: P63321
• Cytogenetics: 13 6.05 cM
• Gene Summary: Multifunctional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. Key regulator of LPAR1 signaling and competes with GRK2 for binding to LPAR1 thus affecting the signaling properties of the receptor. Required for anchorage-independent proliferation of transformed cells (By similarity). The RALA-exocyst complex regulates integrin-dependent membrane raft exocytosis and growth signaling (PubMed:20005108). During mitosis, supports the stabilization and elongation of the intracellular bridge between dividing cells. Cooperates with EXOC2 to recruit other components of the exocyst to the early midbody (By similarity).[UniProtKB/Swiss-Prot Function]