Ecd (NM_027475) Mouse Untagged Clone

CAT#: MC201188

Ecd (untagged) - Mouse ecdysoneless homolog (Drosophila) (Ecd), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ecd
• Synonyms: 5730461K03Rik; Hsgt1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC013470 sequence for NM_027475
  GAGCCTCACGACTTGCAGGCGTTCAGGCGTGGATCAGGGCCCAAGCGAACGCGAGGACCCAGCATGGGCC GGGATTGGGCGGTTGGTGGTCGCCTCTGACCAAGTGTCCTGCTGGAAGAGGCAGCCAAGGAAAGGTGCCA GAGCAGAGGCGCACGAGAGAGCTATTGAAAGGGATCGCTTTACTTTTGTCAGGTGGAAGAAGAGCGTCTA GTTTGGTGAAGGAGCACTATACCGCGCCAGGACAGTGCTCCTGGTGGCTCCTCTTCTGTACTCTCGGAGA AGAATGGAGGGCAGTGGAAAGCTCGCAATGGTAGAGGATGCTGTGGAGTACCATCTCTTCCTGATACCAG ATAAAGCCAGAGGCACAGAAGAGCACAGAGAGATTCTTCAGAAGTACATTGAACGGATAATGACCCAGTT CGCACCCATACTGGTCCCTTATATCTGGCAGAATCAGCCTTTCAATCTCAAATACAAGCCTGCAAAAGGA GGTGTTCCTGCTCATATGTACGGCATGACAAAGTTTGGAGATAACATTGAGGATGAATGGTTTATTGTTT ATGTAATAAAGCAGATTACAAAGGAATTTCCAGAGTTAGTGGCCAGGGTTGAAGACAATGATGGTGAGTT CCTGTTAATAGAAGCGGCCGACTTTCTCCCTAAGTGGTTGGACCCTGATAACAGTGCCAACAGGGTGTTT TTTCATCATGGGGAGCTGTGCATTATTCCTGTGCCGAGGAAATCTGAAAGGATACCATGGTTACCCATGA CACCACCAACAATCCAGCAAGCTTTGAGTATAATCTCGGCACACCCAGAAGCTGTTTTGGCTTCAGAATC TATACAAGCTGCTGTGGACAGGCGCGTCAGTGGGTACCCAGAAAGGGTCGAAGCATCCCTCCATCGAGCA CACTGCTTCCTTCCAGCCGGCATTGTGGCGGTGCTCAAGCAACAGCCCAGGCTGCTGTCGGCAGCCGTCC AGGCCTTCTACCTACGGGACCCCATTGACCTAAGAGCCTGTCGTGTCTTCAAGACATTTTTGCCTGAAAC ACGAATAATGGCCTCGGTCACTTTCACGAAATGTCTGTACGCACAGCTGGTACAGCAGAAGTTTGTGCCA GACCGGCGGAGTGGATACGGGCTCCCACCTCCATCACATCCCCAGTACCGAGCTTATGAACTGGGCATGA AGTTGGCTCATGGATTTGAGATTTTGTGCTCCAAATGTAGCCCACATTTTTCTGACTCCAGGAAGTCTCT TGTGACTGCCTCACCGCTCTGGGCCAGCTTCCTTGAAAGTCTGAAAAGGAATGACTACTTTAAGGGACTG ATGGACGGCTCTGCGCAGTACCAGGAGAGACTAGAAATGGCAAAGAACTATTTCCAGCTCTCTATACACC GGCCAGAAAGTTCACTTGCTATGAGCCCAGGCGAAGAGATCCTAACCGTGTTACAGACACAGCCATTTGA CGTCGCCGAGCTCAAGACAGAAGAAGCTGACCTTCCTCCGGAGGACGATGACCAGTGGTTAGACCTCTCA CCAGACCAGTTAGACCAGCTACTGCAAGATGCTGCGGGCAGAAAAGAATCCCAGCCCGGTCCCCAGAAGG AGGAGCTGCAGAACTATGATGTAGCGCAGGTCTCAGACAGCATGAAGGCCTTCATCTCCAAAGTGTCTAG CCACAAGGGGGCAGAGCTGCCCAGAGACCCTTCGGAAGCTCCAATCACTTTTGATGCAGATTCTTTTCTG AATTATTTTGATAAGATTTTAGGAGCAAAGCCTCAGGAGTCAGACTCTGAGGATGACCCGGGTGAGGAGG ACGTGGAAGGTGTAGACAGTGATGATGACGTGGGCTTTGAGGCTCAGGAGTCTGAGTCTCTGAAAGGAGC CCTTGGCAGCCTCAAGTCCTACATGGCCCGGATGGACCAGGAACTGGCACACACCAGCATGGGCAGAAGC TTCACCACGCGGGAGCGATTGAATAAGGACCCTCCATCCCACACTGCAAATGACAATTCAGATGAAGAAG ATTCTGGTGCAGGAGATTGCGCTGTTGAAGCAGTGGATGTAGACCTGAACCTGATTTCAAATATACTGGA ATCCTACAGCTCCCAAGCGGGCCTGGCAGGACCTGCTTCCAATCTGTTACACAGCATGGGTGTGCGACTG CCCGACAACGCTGATCACAACCCCCAAGTCAGCCAGTGAAGGACTAGCCTGCACATCAGCCACCCTTCTC AGGAATAAACGACTCTTCATAAGCACGCCTTTAATCCCAGCACTCGGGAGACAGAGGCAGGCGGATTTCT GAGTTCGAGGCCAGCCTGGTCTAGAAAATGAGTTCCAGGAGAGCCAGGGCTACACAGAGAAACCCTGTCT CAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_027475
• Insert Size: 1926 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC013470, AAH13470
• RefSeq Size: 2407 bp
• RefSeq ORF: 1926 bp
• Locus ID: 70601
• UniProt ID: Q9CS74
• Cytogenetics: 14 A3
• Gene Summary: Regulator of p53/TP53 stability and function. Inhibits MDM2-mediated degradation of p53/TP53 possibly by cooperating in part with TXNIP. May be involved transcriptional regulation. In vitro has intrinsic transactivation activity enhanced by EP300. May be a transcriptional activator required for the expression of glycolytic genes (By similarity). Involved in regulation of cell cycle progression (PubMed:26711270). Proposed to disrupt Rb-E2F binding leading to transcriptional activation of E2F proteins (PubMed:19640839). The cell cycle -regulating function may depend on its RUVBL1-mediated association with the R2TP complex. May play a role in regulation of pre-mRNA splicing (By similarity).[UniProtKB/Swiss-Prot Function]