Impact (NM_008378) Mouse Untagged Clone

CAT#: MC201164

Impact (untagged) - Mouse imprinted and ancient (Impact), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Impact
• Synonyms: E430016J11Rik
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC020524 sequence for NM_008378
  CGGACGCGTGGGCTCTTTCGGATTTGGGCATTTGGAGCTGGTGTCGCAGATCCTGAGCACCATGGCTGAA GAGGAAGTAGGGAACAGCCAGAGGCAGAGTGAAGAAATCGAAGCAATGGCAGCCATTTATGGCGAGGAGT GGTGTGTCATTGATGAAAATGCCAAAATATTTTGTATTAGAGTCACTGACTTCATGGATGACCCCAAATG GACACTTTGTTTACAGGTGATGTTGCCAAGTGAGTACCCGGGTACAGCACCGCCTTCTTATCAGCTGAAC GCTCCCTGGCTGAAAGGGCAAGAACGCGCAGACTTATCGAACAGCCTTGAGGAGATATATGTCCACAACA TGGGTGAATCTATTCTTTACCAGTGGGTGGAGAAAATAAGAGATGCTCTGATACAGAAATCTCAGATAAC CGAGCCAGACCCAGATGTCAAGAAGAAAACTGAAGAGGTTGAGGTTGAGTCCGAAGAAGACCCTATTCTA GAGCACCCGCCGGAAAATCCAGTTAAAACATTGGACCTCAAAATCAGTGAAGAAACTCAACCAGAAACTG AAGAATTGCCTCCAGTTGCTCATGGCGTTCCTATTACTGACCGAAGGAGCACTTTTCAGGCTCATGTGGC CCCTGTCGTCTGTCCTGAGCAGGTGAAACTGGTTCTTGCCAAGCTGTACGAGAATAAGAAGATTGCCAGC GCCACCCACAACATCTATGCCTACAGGATATTCTGTGAGGATAAACAGACCTTCTTGCAGGACTGTGAAG ATGATGGAGAAACAGCTGCTGGGGGCCGCCTTCTTCACCTCATGGAGATTTTGAATGTGAAGAATGTCAT GGTGGTGGTGTCCCGCTGGTATGGAGGGATTCTGCTGGGACCTGACCGTTTCAAACACATCAACAACTGT GCCAGGAACATTCTGGTGGAGAAGAACTTCACGAACACTCCTGACGAGTCAACTAAGAATTTGGGGAAAA AGAAGGTGAAGAAAGACAAGAAGAAGAATGATCATTAAGACGTGAACCTGTGTGAGAGGTTAACTTCCCA GCCACTGTGGACACGTTCCCAGTGGTCATGAGTACATTAAGATAGCACACTGACAGCTCAAGTCAGCCAG TTCATCATGGTTCCACCAGCATTGTGTTTCCTCCTCTGGTTCCACCATTTGCCAGAACTAGAGAATCTAG AACATATTCACATGTGTTTCACATTTATTTGGAAGAACAGCTGTGAGCTTACTTACCATTTCATATAATA CTAGGAAGTGACACATGCACAGTGTTTTCCTGAGCTAAATGTCAATTTCAGTATTGTCGCGTGCCTTTGT TAGGCAGGGAAGACGTGTCTCCAGAGGAATAGAAAGGCCACATGTCTTGCGATAGAAATTGCCATGCTGT TAAGTGAGCATTGCTTTTTCAGCTCCCAGTTGCTTTCAGAGTGGGGGCGCATGGAAGACGGAGGTCTTGG GGAGTCTTTCTCTTGTGATTTCACAAGTGAGTCACGCTTCCTTCCATAGTATTCATCATGGAGTGATAGT TTACGTGAAAACCCGAGTATTCATCTGTGATCATGTGCCAAAGTGATAGTTTACACATTTGCTAGTCCAA GTGTTACTCCTCAGTTGTTTCAAATTGGTGATTGTGGAATTTCAAAAAGTTACTATTTCTTAGTTGTTGC CTTTTGTTGTTTGATTCCTGTAGTGACACACAGTGACCATTCAGTCTCAGTAGTCCTTCCCAGTAGTCCT TCCGTCCCATCCCTTACGTGTGTTGAGGTAACATTCCGGTGTCTCTGAACGACCTGGAAGCACTTCCCTG TGCTCTTCCCTGTGCTCTGGTCAACTCACTTGCAGAACGAGATAGAGGCAGCAGGACACCAGAGGAGCAG GCTGCCCACTGCACCAGTTTGATATACATTTGCCATTTGAACTCTGGCCAGATTTTTTTTTTTTTTAGTT CTGTGATTTTTTTTTTTTTTTTTTTTGAGGAGAGATAGAGGGACAAATCTTTTGATTAGATTAAAAAAAA AACCTCTTTCCCTGCGGAAGTTTTTGTCTAATCTCCTTTTTTAAACACGTGTTTAATTTATTTTTTACAT GCATATTACTTGTAAGAAATAAGACAAAAATTCCCTTTGTGTAGGTAAGCTGTACCTGTTATCTTTCAGA AATAATGCCAACAATGACTAAATTGATCAACGGATCGCAGACTAGTTTGATTCCCCTCCCGAGGAAGAGG CAGCCTTGGGAGAGTGCTGGAGCACACATAGACAGCGTCTGAGGTTGTGCAGGTTTTTTCTCTTAGTTTG TAAGCTTGAAAGATCCACATCCTCACTTGGTATTTTAAATAGGAATCCAGTAGCCAGCTTTACACTAGAG TGGGTTCTGAGACAGAAGTCTTATTTTCAACATTATTTAATGTTGTAAATTATCAAGAGTTATAGGATGA CCTATATGTGTGTTTGGAACACTTACCTCTGTTTCCATGAAATGCTAAGTATATCCTATGTATTAATTGT TACAGAGCTTAGACAGTATTTGCTAAAGATAATGATATAATCTTGACATTTCAGACCTGTGAAAAGAGAT TAAAACACTAAGAATTTTATGCTGCAGAAAAAAGGAGCATAAAATATTTTCCTAGCACACTGGATTAGAA TTTCTTTCCTGGAGTTTTGTCTAAAATGTATTAGTGTTTACAGAAGAAAAGACAATTTTTTATATCTCTG ATCTCATTTTCCTTCACTTAAAAATTACGTTTCCCCATTTTACAAGATTGCTAACCTCATTTTGTGGGTT GAGCTCCTTAACCTTCGCTGCAGGTGGGAGGGGAGACCCCAGTTCCTCACTTCTAACTCCTGAAATTGCG TATTTCACATGCCATTAGTGCAAAGTAATGATAGCATAGTTTTCTCTATTGAAGTAGAGAAAATCATATG TAGAGAATTTTCTAGTTCTCCCAATTATAACTTTGTAAAGCCAGGCGTCTGCTGTGGCCTTCTCTATATT TTATTAGTTGACATGTTCTCCACATGCCAGTATCTATGAGCAAATGGCATCCACGTAGACGGGCAGAGCA GAAGCTGCTAGTGAGGTGTCTGTCAGAGACAGGCTGTTGGAAGGAAGCACTGATACAGGACAGATAAAGC AGATACTGTTTACAGGGCAGGTGAGTGATACTGTAATCACTTCAGTGAATTGTAATCACATCCAGTCCAC TAAAGAGATGACCATAAATGGTGTATACAGTTTTTATTTTTTATTATGCATGATTTTGTATATATGGCTA TTTTTCTTTCCGTAAAAATGGTATTAAACCGTATATACTGTTTTGTATCCTACATATTTCATATAGAAGT ATATTGTTAACATTTTCCCATATCAATAAATATTCTTCTATGGCTTAAAAAAAAAAAAAAAAAAAAAAAA AAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_008378
• Insert Size: 957 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC020524, AAH20524
• RefSeq Size: 3436 bp
• RefSeq ORF: 957 bp
• Locus ID: 16210
• UniProt ID: O55091
• Cytogenetics: 18 A1
• Gene Summary: Translational regulator that ensures constant high levels of translation upon a variety of stress conditions, such as amino acid starvation, UV-C irradiation, proteasome inhibitor treatment and glucose deprivation. Plays a role as a negative regulator of the EIF2AK4/GCN2 kinase activity; impairs GCN1-mediated EIF2AK4/GCN2 activation, and hence EIF2AK4/GCN2-mediated eIF-2-alpha phosphorylation and subsequent down-regulation of protein synthesis (PubMed:15937339, PubMed:23447528, PubMed:24333428). May be required to regulate translation in specific neuronal cells under amino acid starvation conditions by preventing GCN2 activation and therefore ATF4 synthesis (PubMed:15937339, PubMed:23447528). Through its inhibitory action on EIF2AK4/GCN2, plays a role in differentiation of neuronal cells by stimulating neurite outgrowth (PubMed:23447528).[UniProtKB/Swiss-Prot Function]