Raf1 (NM_029780) Mouse Untagged Clone

CAT#: MC201162

Raf1 (untagged) - Mouse v-raf-leukemia viral oncogene 1 (Raf1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Raf1
• Synonyms: 6430402F14Rik; AA990557; BB129353; c-Raf; Craf1; D830050J10Rik; Raf-1; v-Raf
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC015273 sequence for NM_029780
  CCCACGCGTCCGCCGCCATCTTGGATGGCGGGAGTTAGAGCCGAGCGGACTTGAGGCGGGAAGGCTTCTC TGTTGTTCTCCGCAGGCCCTCGGACGGGCCAGCTGGCCGAGCTTTGGGGCGCGTGCCCTGAGGCGCGGGG CGCTAGTGCAGCGATGCGGGGCCTCCCCGGGGCCCCGTCCCCCGGGCTGGGGACACGCCGAATGTGACCG CAACCAACGCGTTCTCTCAACCACCGCTGCCGCTGCACGACGGGACCCGGCACTGCCCGGCTCCCTCAGG TATAAGTATTGTTTAAACTGCATCAATGGAGCACATACAGGGAGCTTGGAAGACGATCAGCAATGGCTTT GGACTCAAAGATGCGGTGTTTGATGGCTCCAGCTGCATCTCCCCTACCATTGTTCAGCAGTTTGGCTATC AGCGCCGGGCCTCAGATGATGGCAAGCTCACGGATTCTTCTAAGACAAGCAATACTATCCGGGTTTTCTT GCCGAATAAGCAAAGGACTGTGGTCAATGTGCGGAATGGAATGAGCTTACATGACTGCCTTATGAAAGCT CTGAAGGTGAGAGGCCTGCAGCCAGAGTGCTGTGCAGTGTTCAGACTTCTCCAGGAACACAAAGGTAAGA AAGCACGCTTAGATTGGAACACCGATGCCGCCTCTCTGATTGGAGAAGAACTGCAAGTGGATTTTTTGGA TCATGTTCCACTCACAACTCACAACTTTGCTCGGAAAACGTTCCTGAAGCTTGCATTCTGTGACATCTGT CAGAAGTTCCTGCTAAATGGATTTCGATGTCAGACTTGTGGCTACAAGTTTCATGAGCACTGTAGCACCA AAGTACCTACTATGTGTGTGGACTGGAGTAATATCAGACAGCTCTTGCTGTTTCCAAATTCCACTGTTGG TGACAGTGGAGTCCCAGCACCACCTTCTTTCCCAATGCGTCGGATGCGAGAATCTGTTTCCCGGATGCCT GCTAGTTCCCAGCACAGATACTCTACACCCCATGCCTTCACTTTCAACACCTCCAGCCCTTCCTCAGAAG GTTCCCTGTCCCAGAGGCAGAGGTCAACGTCCACTCCCAATGTCCACATGGTCAGCACCACCCTGCATGT GGACAGCAGGATGATTGAGGATGCAATTCGAAGTCACAGTGAATCAGCCTCACCTTCAGCCCTGTCCAGC AGCCCAAACAACCTGAGTCCAACAGGCTGGTCACAGCCCAAAACCCCTGTGCCAGCACAAAGAGAGCGGG CACCAGGATCTGGGACCCAGGAAAAAAACAAAATTAGGCCTCGTGGGCAGAGAGACTCGAGTTATTACTG GGAAATAGAAGCCAGTGAGGTGATGCTGTCTACTCGGATCGGGTCAGGTTCCTTTGGCACTGTGTACAAG GGCAAGTGGCATGGAGATGTTGCAGTAAAGATCCTAAAGGTGGTTGACCCAACTCCAGAGCAACTTCAGG CCTTCAGGAACGAGGTGGCTGTTTTGCGCAAAACACGGCATGTTAACATCCTGCTGTTCATGGGGTACAT GACAAAGGACAACCTGGCGATTGTGACTCAGTGGTGTGAAGGCAGCAGTCTCTACAAACACCTGCATGTC CAGGAGACCAAATTCCAGATGTTCCAGCTAATTGACATTGCCCGACAGACAGCTCAGGGAATGGACTATT TGCATGCAAAGAACATCATCCACAGAGACATGAAATCCAACAATATATTTCTCCATGAAGGCCTCACGGT GAAAATTGGAGATTTTGGTTTGGCAACAGTGAAGTCACGCTGGAGTGGTTCTCAGCAGGTTGAACAGCCC ACTGGCTCTGTGCTGTGGATGGCCCCAGAAGTAATCCGGATGCAGGATGACAACCCGTTCAGCTTCCAGT CCGACGTGTACTCGTACGGCATCGTGCTGTACGAGCTGATGGCTGGGGAGCTTCCCTACGCCCACATCAA CAACCGAGACCAGATCATCTTCATGGTAGGCCGTGGGTATGCATCCCCTGATCTCAGCAGGCTCTACAAG AACTGCCCCAAGGCAATGAAGAGGTTGGTGGCTGACTGTGTGAAGAAAGTCAAAGAAGAGAGACCTTTGT TTCCCCAGATCCTGTCTTCCATCGAGCTGCTTCAGCACTCTCTGCCGAAAATCAACAGGAGCGCCTCTGA GCCTTCCCTGCATCGGGCAGCTCACACTGAGGACATCAATGCTTGCACGCTGACTACATCCCCAAGGCTA CCAGTCTTCTAGCTGATGATGTAGCTGTTCTTAGGCCACCAGGGGACGAAGAAGAGTCAGCAGGCACCAC TTTCTGTTTCCTTGGGGGCAGAATGCATGTTTTTGGAAAAGCTGCTGCTGCTAAGGACCTAGACTACTCA CAGGGCCTTAACTTCATATTGCCTTCTTTTCTACCCCTCCTGCCCTGGAAATGGAAGCTGTCCACCAAGA AAGCCTGCTCAGCTCCAGAGGTATACAAGTCAGCTGAGTATTTGTAGGGCAAGTGGCCTTGGAGAAGGTA GGGCACTCCGGCTGCTGCAGGGACATGCAATTGGGAACTTGGCTCCTTGAGCCTATATGGACAGTGGTGC AGTGCCAGTTTTGCACATGGAGTCCTGGCCACCTGGGGGAGCCTGCTTTGGTACTACAGAACTTTGTGGA CAAACTGTGTCTAAGGTGTCCTGTGTGGATGGCTTTCCAAGCGCGGTGCTCCACCTTTGGCAGCCTCCCA CGTGCTGAATCTGTCTTCCAGGAGCTGCCCCTATGGGGTGGGCCGCGGCCCAACCCTATCTGTAGCCACA TCCTTGTCTGTAAGAAAGCCAGGAATACAGGTTTTCTTAATGATTTTGGGTTTTAATTTTGTTTTTATTG AGCCAATACAGTTGGTTCCTTATGTTATTTTAATAAAATAAATTAAATTTAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_029780
• Insert Size: 1947 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC015273, AAH15273
• RefSeq Size: 2935 bp
• RefSeq ORF: 1947 bp
• Locus ID: 110157
• UniProt ID: Q99N57
• Cytogenetics: 6 53.62 cM
• Gene Summary: Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation (By similarity). Regulates Rho signaling and migration, and is required for normal wound healing.[UniProtKB/Swiss-Prot Function]