Car1 (NM_009799) Mouse Untagged Clone

CAT#: MC201127

Car1 (untagged) - Mouse carbonic anhydrase 1 (Car1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Car1
• Synonyms: AW555628; Ca1; Car-1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC011223 sequence for NM_009799
  GCAGTTAGTCATTTCACATATCATTTCTCTTTACAGGAATCACAACCTAAATAAGAGAAAATGGCAAGTG CAGACTGGGGATATGGAAGCGAAAATGGTCCTGACCAATGGAGCAAGCTGTATCCCATTGCCAATGGAAA CAACCAGTCTCCTATTGATATTAAAACCAGTGAAGCCAATCATGACTCCTCTCTGAAACCACTCAGCATC TCCTATAATCCTGCAACTGCCAAAGAAATTGTTAACGTGGGACATTCTTTCCATGTAATTTTTGATGACA GTAGCAACCAATCTGTTCTGAAAGGTGGCCCTCTTGCTGATAGCTATCGGCTCACTCAGTTCCATTTTCA CTGGGGCAACTCAAACGACCATGGCTCTGAGCACACCGTGGATGGAACTAGATATTCTGGAGAGCTTCAC TTAGTTCACTGGAATTCTGCAAAGTACTCCAGTGCTTCTGAAGCCATCTCCAAGGCTGATGGCCTGGCAA TCCTTGGCGTTTTGATGAAGGTTGGTCCAGCCAACCCAAGCCTGCAGAAAGTACTTGATGCTCTAAACTC AGTTAAAACTAAGGGAAAACGAGCCCCATTCACAAATTTTGACCCATCCAGTCTGCTTCCTTCATCTCTG GATTACTGGACCTACTTTGGCTCTCTGACTCACCCTCCTCTTCATGAAAGTGTGACCTGGGTGATCTGCA AGGATAGCATCAGTCTAAGCCCAGAGCAGCTGGCCCAGCTCCGTGGTCTTCTGTCAAGTGCAGAGGGAGA GCCTGCAGTTCCAGTTCTGAGCAACCACCGTCCACCCCAACCCCTGAAGGGCAGAACAGTCAGAGCCTCA TTTTGAGTCCCAGCAAGGAATGAGTCCTCAATTAGGACCTGGCCCCCTCTACAGCAGCATCCAATAAAGG GATCACGATTAAGAAACAGAATTATGTCAGTGCTAAGACAGTATACCTGCAAATTTAATCCACAGAACTA AAATGCTTTCATTTTAATTCCTGATACTTAATGCAAATACTCTATAAGTTTGCCTACTGTAATTTTTATG CATTCGTTTTTGCTTAAATACACCTTTTTGTGGAATCGTTCATAATTACAACTAAGTGATTCATTCTCTT TCTGCTCTCTTCACTTTAAATAAACCAATAAAATGGCTTTACCTCCTAAAAAAAAAAAAAAAAAAAAAAA AAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_009799
• Insert Size: 786 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC011223, AAH11223
• RefSeq Size: 1195 bp
• RefSeq ORF: 786 bp
• Locus ID: 12346
• UniProt ID: P13634
• Cytogenetics: 3 3.18 cM
• Gene Summary: Reversible hydration of carbon dioxide.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript. Both transcript variants (1 and 2) encode the same protein. This variant represents use of an upstream long terminal repeat (LTR) promoter and exon sequence, and results in erythroid expression. (PMID: 2571923).