Alox8 (NM_009661) Mouse Untagged Clone

CAT#: MC201095

Alox8 (untagged) - Mouse arachidonate 8-lipoxygenase (Alox8), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Alox8
• Synonyms: 8-L; 8-LOX; 8S-; 8S-LOX; 15-LOX-2; 15-LOX-B; Alox; Alox15b
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC015253 sequence for NM_009661
  CAGCTTGCAGTAGAGAGCTAAACTGGTCAGGAGGATGGCGAAATGCAGGGTGAGAGTATCCACGGGGGAA GCCTGTGGGGCTGGCACATGGGACAAAGTGTCTGTCAGCATCGTGGGAACCCACGGAGAGAGCCCCTTAG TACCTCTGGACCATCTGGGCAAGGAGTTCAGCGCCGGTGCTGAAGAAGACTTCGAGGTGACGCTTCCCCA GGACGTAGGCACTGTGCTGATGCTGCGAGTCCACAAAGCACCCCCGGAAGTGTCCCTCCCGCTTATGTCT TTCCGTTCTGATGCCTGGTTCTGCCGCTGGTTCGAGCTGGAGTGGCTACCTGGGGCTGCACTCCACTTCC CCTGTTATCAGTGGCTGGAAGGGGCGGGGGAGCTGGTGCTGAGAGAGGGAGCAGCAAAGGTGTCCTGGCA AGACCATCACCCTACACTGCAGGATCAGCGCCAGAAGGAGCTTGAGTCCAGGCAGAAGATGTACAGCTGG AAGACTTACATTGAAGGTTGGCCTCGCTGCCTTGACCACGAGACTGTGAAAGACTTGGACCTCAACATCA AGTACTCTGCGATGAAGAATGCCAAACTCTTCTTTAAAGCCCACTCCGCGTATACGGAGCTGAAAGTCAA AGGGCTCCTGGACCGCACAGGACTCTGGAGGAGTCTGAGGGAGATGAGAAGGCTGTTTAACTTCCGCAAG ACTCCAGCAGCAGAGTATGTGTTTGCACACTGGCAGGAAGATGCCTTCTTCGCCTCCCAGTTCCTAAATG GCATCAACCCGGTCCTGATTCGCCGCTGTCACAGTCTCCCAAACAACTTCCCGGTCACTGATGAAATGGT GGCCCCAGTGCTGGGCCCTGGAACCAGTCTGCAGGCTGAGTTGGAGAAGGGCTCCCTGTTCTTGGTGGAT CATGGCATTCTTTCTGGAGTCCACACCAACATCCTCAATGGAAAGCCTCAGTTCTCTGCAGCCCCGATGA CCCTGTTACACCAGAGCTCAGGGTCCGGACCCCTGCTTCCCATTGCCATCCAGCTCAAACAGACTCCCGG GCCAGACAACCCCATCTTCCTGCCCAGCGATGACACGTGGGACTGGTTGCTGGCCAAGACCTGGGTTCGC AATTCTGAGTTTTACATCCATGAGGCTGTCACACATCTGCTGCATGCCCATCTGATTCCAGAAGTCTTTG CCTTGGCCACATTACGTCAGCTGCCTAGGTGTCACCCTCTCTTCAAGCTATTGATTCCTCACATTCGGTA CACACTGCACATCAACACGCTTGCCCGGGAGCTGCTCGTTGCCCCTGGGAAGTTGATAGACAAGTCCACA GGCCTTGGCACTGGGGGATTCTCTGACCTGATAAAGAGAAACATGGAGCAGCTGAACTACTCTGTCCTGT GTCTCCCTGAAGATATCCGAGCCCGAGGTGTGGAAGACATCCCAGGCTACTATTACCGAGATGATGGGAT GCAGATCTGGGGGGCAATAAAGAGCTTTGTCTCTGAAATAGTCAGCATCTACTATCCAAGTGACACATCC GTCCAAGATGACCAAGAGCTCCAGGCCTGGGTGAGGGAGATCTTCTCTGAGGGCTTCCTCGGCCGAGAAA GCTCAGGTATGCCCTCCTTGTTGGATACCCGGGAAGCCCTGGTCCAGTATATCACCATGGTGATATTCAC CTGCTCAGCCAAGCATGCAGCTGTCAGTTCAGGCCAGTTCGACTCTTGTGTTTGGATGCCCAATCTGCCA CCTACCATGCAGCTACCACCACCTACTTCCAAAGGCCAGGCCCGGCCTGAGAGTTTCATAGCCACGCTCC CAGCAGTTAATTCGTCAAGTTATCACATCATTGCTCTCTGGCTGCTAAGCGCAGAACCTGGGGACCAAAG GCCCCTGGGCCACTATCCAGATGAACACTTCACAGAGGATGCCCCCCGGCGAAGCGTGGCTGCCTTCCAG AGAAAGCTGATCCAGATCTCCAAGGGCATCAGGGAGAGGAACCGAGGCCTGGCACTGCCCTACACCTACC TGGATCCTCCCCTCATTGAGAACAGTGTCTCCATCTAACATCTTGGAGAAGACAGTCCTGTGTGACATAT AGAACTCTTGACCATGCCTCTCCAGGCTAAGTCCCCGTATGCTTCTCCTGGACAACCAAGCCCCATCTTA CACACACACACACACACACACACCTAATAAAATCGAAACAGAAAAACCTAAACTCCCACAGAAGGCAAGA TCTCACACAGCAGAGAGCCATCCAAATGTTTGGAGACCCTGAGCTTCAGCTCTGATTAACGGCTTTGCTG GTTTGCTTTGCTTTCTATTCCATTAACCATGGACGGTAACAGAAAGCACAGAACCCTGGTTCACTGCACA AAGCCACTGAGATCTCACCCTCACCTGACACAAAGGCAGCTATCATACAGGCTTATCAGGAACACAGGAA TTTGTCCAATCAAAGCCTACCCACTAGGTCCATCGTGACCTACGACCTCACACTGGCATGCTTTAGCTTT GAGAAGGGATTACTGGAGTCAGGTACGAAGAGAAGGACAGGACGAAGGCATGGCTCCATGTGGAAGAACA TATCTGCTCTTCCAGATGACCAGGGTAGCTCACAGCCATGTGTCATTCTAACTCCAGAGGTCTCTAGTGG CCATGAAGACTCCAGGCATTCAGGGGATATACCAGTAGACACCAAAATTATACTTTTTAAGAGAGAGGAA TGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCAGAGATCCTGAGTTCAATTCCCA GCAACCACATGGTGGCTCACAACCATCTGTAATGGGATTCGATGCCCTCTTCTGGCGTGTCTGAAGACAG CGACAGTGTATGCACATATATAAAATAAATAAATCTTTAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAA AA
• Restriction Sites: RsrII-NotI
• ACCN: NM_009661
• Insert Size: 2034 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC015253, AAH15253
• RefSeq Size: 2942 bp
• RefSeq ORF: 2034 bp
• Locus ID: 11688
• UniProt ID: O35936
• Cytogenetics: 11 B3
• Gene Summary: This gene belongs to the lipoxygenase (LOX) gene family whose members encode enzymes that catalyze the addition of molecular oxygen to polyunsaturated fatty acids (PUFAs) to yield fatty acid hydroperoxides. The encoded enzyme preferentially metabolizes arachidonic acid to yield 8-hydroxyeicosatetraenoic acid (8-HETE), while metabolizing linoleic acid less efficiently. The gene may also function as a tumor suppressor. This gene is located in a cluster of related genes that spans approximately 75 kilobases on chromosome 11. [provided by RefSeq, Jan 2013]