Gramd1a (BC018554) Mouse Untagged Clone

CAT#: MC200835

Gramd1a (untagged) - Mouse GRAM domain containing 1A (cDNA clone MGC:19375 IMAGE:2646082), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gramd1a
• Synonyms: 1300003M23Rik; AI415002; AW547240; D7Bwg0611e
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC018554
  CTCTGGCTTCTGAGTCAGAACCAGGCACCATGGTGGAGAAGGGGTCTGACAGCTCCTCTGAGAAAAGTGG CGTGTCGGGGACACTCAGCACCCAGAGCCTGGGCAGCCGGAACTTCATCCGCAACAGTAAGAAGATGCAG AGCTGGTACAGTATGCTGTGTCCCACATACAAACAACGCAACGAGGACTTCCGGAAACTGTTCAGCAAAC TTCCTGAAGCAGAGCGCCTCATCGTGGATTACTCCTGCGCCCTGCAGAGGGAGATCCTCCTCCAGGGCCG CCTCTACCTCTCTGAGAACTGGATCTGTTTCTACAGCAACATCTTCCGCTGGGAGACCACAATTTCCATC CAGTTGAAGGAAGTGACCTGTCTGAAGAAGGAGAAGACGGCTAAGCTCATCCCCAATGCCATTCAGATCT GTACTGAGAGTGAGAAGCATTTCTTCACTTCCTTCGGGGCCCGAGACCGCTGCTTCCTCCTCATCTTCCG TCTCTGGCAGAATGCGCTGCTTGAAAAGACACTGAGTCCTCGAGAGCTTTGGCACCTGGTCCATCAGTGC TATGGCTCGGAGCTGGGCCTCACCAGTGAAGACGAAGACTATGTCTGCCCCTTGCAACTGAATGGTCTTG GGAGTCCCAAAGAGGTGGGAGACATGATCGCACTCAGCGACATCAGTCCCTCGGGGGCGGCTGACCACAG CCAGGAGCCAAGCCCTGTGGGTTCAAGGCGTGGCCGGGTCACCCCAAACCTCTCTCGAGCCAGCAGTGAT GCAGACCATGGGGCCGAGGAGGACAAGGAAGAACAGACGGACAGTCAGCTGGATGCCTCCTCCAGCCAGA CAGTGACCCCAGTAGCAGAACCGCTGAGTTCTGAGCCGACACCACCAGATGGGCCCACTTCCTCCTTGGG TCCCTTGGATCTGCTGTCTAGAGAGGAACTATTGACAGACACGAGTAACTCGTCCTCATCCACTGGGGAG GAAGGTGACCTGGCTGCACTGCTGCCTGACCTCTCCGGCCGGCTCCTCATCAACTCCGTCTTCCACATGG GTGCTGAGCGTCTGCAGCAGATGCTCTTCTCGGACTCTCCGTTCCTGCAAGGCTTCCTGCAGCAGCGCAA GTTCACAGATGTGACCTTGAGCCCTTGGAGCAGTGACAGCAAGTGCCACCAGCGCAGAGTCCTGACCTAC ACCATCCCCATTAGCAACCAGCTAGGCCCCAAGAGCGCCTCTGTGGTGGAGACACAGACGCTGTTCCGGC GCGGCCCACAGGCGGGCGGGTGCGTGGTGGACTCTGAGGTGCTCACCCAGGGCATCCCGTACCAGGACTA CTTCTACACTGCCCACCGATACTGTATCCTGGGTCTGGCCCGGAACAAGGCCCGGCTTCGTGTATCCTCA GAGATCCGCTACCGAAAGCAACCGTGGAGCCTCGTAAAGTCGCTCATTGAGAAGAACTCGTGGAGCGGCA TCGAAGATTATTTCCACCACCTGGATCGAGAACTCGCCAAGGCAGAAAAGCTGTCCCTGGAGGAAGGAGG GAAAGATACACGTGGACTACTGTCAGGCCTGCGGAGGAGGAAGCGGCCCCTGAGCTGGAGGGGGCACAGA GATGGGCCTCAGCACCCAGACCCAGACCCCTGCACCCAGACCAGCATGCACACCTCAGGTTCCCTCAGCT CTCGCTTCTCGGAACCATCTGTGGATCAGGGCCCTGGGGCAGGCATCCCCAGCGCCCTGGTTCTCATCAG CATTGTCCTCATCGTTCTCATCGCCCTCAATGCCCTCCTCTTCTACCGTCTCTGGTCTCTGGAGAGAACA GCCCATACCTTTGAGTCCTGGCACAGCCTGGCACTGGCCAAGGGCAAGTTCCCCCAGACAGCCACGGAGT GGGCGGAGATCTTGGCTCTGCAGAAGCACTTCCACAGCGTTGAGGTGCACAAGTGGAGGCAGATCCTTCG AGCATCCGTGGAGCTCCTGGATGAGATGAAGTTCTCCTTGGAGAAGCTACATCAAGGAATTACTGTCCCG GATCCTCCCCTGGACACTCAGCCACAGCCTGATGACAGTTTTCCCTGAGGACTCCTGATTGAAAAGCCAC ACCCCTAAACGGACAGAAGGACACATGGAACCCTGGCAGTCACTGCTGGCACAGTGCGAATGCCGCCGGG AGGTTCCTGCCTGCCCCTCACTGTGGCCCTGCCAAGAGCCACACAGACCTGGGGACCATAGAACCAAGAT GCACTTTAGATCTGTGTGAGCGATTCCAGCTGCCACTGCCTGCTCAGCCAGGGAGCTGGCCTGGTCTTTG GCAGACTCCCCCTCCAACTAACTTATTTTGCCCAGCTGGAGTTGTGGGGGGCACCTTTTGGGGTGCACAA GAACCCTCTGGGTTTAATGTATTTATTTGGGAATAACGACCCCAATAAAGGGTTGGAAATGGCAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: BC018554
• Insert Size: 1944 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC018554, AAH18554
• RefSeq Size: 2513 bp
• RefSeq ORF: 1944 bp
• Locus ID: 52857
• Cytogenetics: 7 19.3 cM
• Gene Summary: Cholesterol transporter that mediates non-vesicular transport of cholesterol from the plasma membrane (PM) to the endoplasmic reticulum (ER) (PubMed:30220461). Contains unique domains for binding cholesterol and the PM, thereby serving as a molecular bridge for the transfer of cholesterol from the PM to the ER (PubMed:30220461). Plays a crucial role in cholesterol homeostasis and has the unique ability to localize to the PM based on the level of membrane cholesterol (PubMed:30220461). In lipid-poor conditions localizes to the ER membrane and in response to excess cholesterol in the PM is recruited to the endoplasmic reticulum-plasma membrane contact sites (EPCS) which is mediated by the GRAM domain (PubMed:30220461). At the EPCS, the sterol-binding VASt/ASTER domain binds to the cholesterol in the PM and facilitates its transfer from the PM to ER (PubMed:30220461). May play a role in tumor progression (PubMed:27585821).[UniProtKB/Swiss-Prot Function]